Production of Adenoviral Vectors in 293 Cells: A Case Study of the Adaptation of Attached Cells to Grow in Suspension

Negrete, Alejandro; Ling, Tau Chuan; Lyddiatt, Andrew

doi:10.2174/1874070700802010029

Cited by 1 publication

(1 citation statement)

References 36 publications

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“…The cell growth and post infection time coincided with the 72h stage at which the cells can grow without aeration. Without the CO 2 supply to the culture of suspension adapted 293 cells, pH control became more difficult, especially because of the low buffering capacity of the 293 SFM II determined previously [35]. For this reason it was necessary to investigate the addition of a more robust buffer system to the medium without impacting upon the cell density or viability of the suspension adapted 293 cells.…”

Section: Effect Of a 5% Co 2 Atmosphere Upon 293 Cellsmentioning

confidence: 99%

Effect of Pluronic F-68, 5% CO, Atmosphere, HEPES, and Antibiotic-Antimycotic on Suspension Adapted 293 Cells

Negrete¹,

Ling²,

Lyddiatt³

2008

TOBIOTJ

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Abstract:The influence of different parameters upon cell culture of serum-free adapted 293 cells including the surfactant Pluronic F-68, carbon dioxide (5% CO 2 atmosphere), buffer HEPES and antibiotic-antimycotic has been explored. A defined serum-free medium (SFM) formulated without human or animal origin components from Invitrogen was used to grow the suspension adapted 293 cells. For all cell culture parameters cell density and viability of the suspension adapted 293 cells were monitored. The results indicated that the PF68 concentrations ranging from 0.05% to 0.2% can be used in the culture of the suspension adapted 293 cells since no negative effect upon either cell density or viability was detected. This will minimize the formation of aggregates during cell culture. It was demonstrated that neither the cell density nor the viability of the suspension adapted 293 cells were affected by the 5% CO 2 atmosphere at the inoculation cell densities evaluated. The use of the buffer HEPES in the cultivation of suspension adapted 293 cells did not cause negative effects upon cell density and viability. The addition of HEPES makes more robust the culture to pH fluctuations. The antibioticantimycotic can be used when needed at concentrations of up to 50 IU/ml for the culture of this particular cell line, with no apparent effect upon cell growth. The results obtained will contribute to a basic understanding of the 293 cell culture in the 293 SFM II and to the process development of their culture in bioreactors for the expression of different products of biotechnology interest.

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Section: Effect Of a 5% Co 2 Atmosphere Upon 293 Cellsmentioning

confidence: 99%