Carboxymethyl cellulose and Pluronic F68 protect the dinoflagellate Protoceratium reticulatum against shear-associated damage

Rodríguez, J.J. Gallardo; Sánchez-Mirón, A.; Camacho, F. Garcı́a; Cerón-García, M.C.; Belarbi, El Hassan; Chisti, Yusuf; Grima, E. Molina

doi:10.1007/s00449-010-0441-7

Cited by 15 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These differences in cell sizes were attributed to the very different turbulence levels that prevailed in the two bioreactor systems, as shown later in this paper. Turbulence, generally quantified as an average shear rate in the culture broth, is well known to affect growth, cell morphology and other characteristics of microalgae so long as its threshold is above some critical value …”

Section: Resultsmentioning

confidence: 99%

“…For certain microalgae, the turbulence intensity, or shear rate in the culture broth, also influences biomass production, although this subject has received no attention in relation to Chlorella spp. Turbulence fields in different configurations of bioreactors can be quite different and, therefore, the type of bioreactor can influence culture productivity quite apart from any differences in light regimes of the bioreactors.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of shear rate, photoautotrophy and photoheterotrophy on production of biomass and pigments by Chlorella vulgaris

Benavente‐Valdés¹,

Méndez‐Zavala²,

Morales‐Oyervides³

et al. 2017

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: This work compared the production of Chlorella vulgaris biomass and pigments (chlorophylls and carotenoids) in photoautotrophic, photoheterotrophic and two-stage photoheterotrophic-photoautotrophic cultures in two types of photobioreactors, a flat-panel airlift (FPA) and a conventional stirred tank (STR) system. RESULTS: The biomass and pigments productivities of photoheterotrophic and photoheterotrophic-photoautotrophic cultureswere much greater than the productivity of photoautotrophic culture in the FPA. The 3.9-fold higher average shear rate values in the FPA relative to the STR affected the size of the algal cells, but not the final biomass concentration. The two-stage photoheterotrophic-photoautotrophic culture in the FPA had nearly 48% higher biomass productivity than the photoheterotrophic culture in the same device but offered no advantage in terms of production of the pigments. CONCLUSIONS:The FPA and the STR offer very different culture environments which affect an algal culture; a two-stage culture was more productive in biomass than a photoautotrophic culture but not for pigments production.Viscosity of the culture broth max Maximum specific growth rate L Density of the culture broth * Correspondence to: J Montañez,

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of shear rate, photoautotrophy and photoheterotrophy on production of biomass and pigments by Chlorella vulgaris

Benavente‐Valdés¹,

Méndez‐Zavala²,

Morales‐Oyervides³

et al. 2017

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…P. reticulatum has been used as a model to better understand some of the aspects of mass culturing these fragile microalgae 5, 12–18. Although approaches for a successful scale‐up of stirred tank photobioreactors for growing this highly fragile dinoflagellate have been reported,6 work is needed on methods for mitigating the damaging effects of turbulence.…”

Section: Introductionmentioning

confidence: 99%

“…Certain shear‐protective chemicals can be added to the culture of sensitive cells to dramatically improve their tolerance to shear stresses 13, 25–27. This allows culture of the otherwise sensitive algae in relatively turbulent conditions in a photobioreactor.…”

Section: Introductionmentioning

confidence: 99%

“…This allows culture of the otherwise sensitive algae in relatively turbulent conditions in a photobioreactor. The dinoflagellate P. reticulatum and other nondinoflagellate microalgae have also been at least partly protected against turbulence‐associated damage by the use of the shear protectant Pluronic F68 (PF68), a surface‐active polymer 5, 13, 28. PF68 has been shown as an effective protectant for animal cells and its protective effect has been ascribed to a reinforcement of the membrane25, 29 by direct interaction with it.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Shear‐induced changes in membrane fluidity during culture of a fragile dinoflagellate microalga

Gallardo

Camacho

Sánchez-Mirón

et al. 2011

Biotechnology Progress

Self Cite

View full text Add to dashboard Cite

The commonly used shear protective agent Pluronic F68 (PF68) was toxic to the marine dinoflagellate microalga Protoceratium reticulatum, but had a shear-protective effect on it at concentrations of ≤ 0.5 g L(-1) . Supplementation of P. reticulatum cultures with PF68 actually increased the fluidity of the cell membrane; therefore, the shear protective effect of PF68 could not be ascribed to reduced membrane fluidity, an explanation that has been commonly used in relation to its shear protective effect on animal cells. Data are reported on the membrane fluidity of P. reticulatum and its response to the presence of PF68 under sublethal and lethal turbulence regimens. The membrane fluidity was found to depend strongly on the level of lipoperoxides in the cells produced under lethal agitation.

show abstract

Cellulose from oil palm empty fruit bunch fiber and its conversion to carboxymethylcellulose

Yimlamai

Choorit

Chisti

et al. 2021

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND Higher‐value products, such as cellulose and carboxymethylcellulose (CMC), may be produced from lignocellulosic agroindustrial residues, such as oil palm empty fruit bunch (EFB) fibers that are presently underutilized or discarded. Recovery of cellulose from EFB and its conversion of CMC are discussed as methods of creating value and reducing the environmental impact of EFB. RESULTS Cellulose was recovered from EFB fibers and converted to water soluble CMC. Cellulose isolation used a two‐step sequential treatment involving peracetic acid (PA) (50 g dry EFB L−1 PA, 35 °C, 9 h) for initial delignification, and then alkaline peroxide (AP) (50 g delignified‐EFB L−1 AP, 45 °C, 12 h). This treatment removed 91.5% of lignin in the original EFB fiber. The recovered solids, designated as cellulose‐EFB, were comprised of 83.7 ± 3.4% cellulose, 5.6 ± 0.3% hemicellulose, 1.7 ± 0.0% lignin, and 1.7 ± 0.1% ash. A two‐step process with an initial alkalization {3 g cellulose‐EFB in 99 mL solvent [10:1 by vol mixture of ethanol (95%) and 35% NaOH], 30 °C, 2 h} followed by etherification was used to convert cellulose‐EFB to CMC sodium salt. The conversion yield was 50% of the theoretical maximum. CONCLUSION The best conditions for preparing CMC from cellulose‐EFB were a 2 h treatment with 35% (wt/vol) NaOH during alkalization at 30 °C, followed by etherification at 55 °C. The CMC had a degree of substitution of 0.16 and a water solubility of 6.8 g L−1 at 30 °C. © 2021 Society of Chemical Industry

show abstract

Carboxymethyl cellulose and Pluronic F68 protect the dinoflagellate Protoceratium reticulatum against shear-associated damage

Cited by 15 publications

References 30 publications

Effects of shear rate, photoautotrophy and photoheterotrophy on production of biomass and pigments by Chlorella vulgaris

Effects of shear rate, photoautotrophy and photoheterotrophy on production of biomass and pigments by Chlorella vulgaris

Shear‐induced changes in membrane fluidity during culture of a fragile dinoflagellate microalga

Cellulose from oil palm empty fruit bunch fiber and its conversion to carboxymethylcellulose

Contact Info

Product

Resources

About