The effect of one injection of Depo-Provera® on the human vaginal epithelium and cervical ectopy

Mauck, Christine; Callahan, Marianne M.; Baker, Jay M.; Arbogast, Kathleen; Veazey, R. S.; Stock, Richard J.; Pan, Zhiying; Morrison, Charles; Chen-Mok, Mario; Archer, David F.; Gabelnick, Henry L.

doi:10.1016/s0010-7824(99)00058-x

Cited by 112 publications

(92 citation statements)

References 13 publications

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“…Although studies using a rhesus macaques model have shown that subcutaneous progesterone administration by implants resulted in vaginal epithelial thinning and increased simian immunodeficiency virus vaginal transmission, studies using human vaginal biopsies have generally not shown an association between hormonal contraception and significant changes in vaginal epithelial thickness and the number of cell layers from biopsy, Langerhans' cell count, or maturation index. [63][64][65] Hence, humans may not respond to exogenous progestins with the dramatic vaginal thinning noted in primates, which is a reassuring finding.…”

Section: Contraceptionmentioning

confidence: 95%

Anatomic and Hormonal Changes in the Female Reproductive Tract Immune Environment during the Life Cycle: Implications for HIV/STI Prevention Research

Venkatesh

Cu‐Uvin

2014

American J Rep Immunol

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Section: Contraceptionmentioning

confidence: 95%

Anatomic and Hormonal Changes in the Female Reproductive Tract Immune Environment during the Life Cycle: Implications for HIV/STI Prevention Research

Venkatesh

Cu‐Uvin

2014

American J Rep Immunol

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“…However, the human and rodent reproductive tracts differ in several respects that may influence Ag uptake and induction of immune responses. In humans, the vaginal epithelium may not be more permeable to Ag during the luteal phase because progesterone does not significantly thin the epithelium (16). Both the human and rodent reproductive tracts contain large numbers of DC (12,17), but the human (and nonhuman primate) reproductive tract contains much greater numbers of CD4 ϩ and CD8 ϩ T cells, macrophages, B lymphocytes, and plasma cells (17)(18)(19)(20)(21)(22)(23)(24).…”

mentioning

confidence: 99%

Differential Induction of Mucosal and Systemic Antibody Responses in Women After Nasal, Rectal, or Vaginal Immunization: Influence of the Menstrual Cycle

Kozlowski

Williams²,

Lynch³

et al. 2002

The Journal of Immunology

204

186

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A cholera vaccine containing killed vibrios and cholera toxin B subunit (CTB) was used to compare mucosal immunization routes for induction of systemic and mucosal Ab. Four groups of women were given three monthly immunizations by the rectal immunization (Rimm) route, nasal immunization (Nimm) route, or vaginal immunization route during either the follicular (V-FPimm) or luteal (V-LPimm) menstrual cycle phase. Nimm was performed with 10-fold less vaccine to determine if administration of less Ag by this route can, as in rodents, produce mucosal Ab responses comparable to those induced by higher dose Rimm or vaginal immunization. Concentrations of Ab induced in sera and secretions were measured by ELISA. None of these routes produced durable salivary Ab responses. Nimm induced greatest levels of CTB-specific IgG in sera. Rimm failed to generate CTB-specific IgA in genital tract secretions. Nimm, V-FPimm, and V-LPimm all produced cervical CTB-specific IgA responses comparable in magnitude and frequency. However, only V-FPimm induced cervical IgA2-restricted Ab to the bacterial LPS vaccine component. V-FPimm, but not V-LPimm, also induced CTB-specific IgA in rectal secretions. Nimm was superior to V-FPimm for producing rectal CTB-specific IgA, but the greatest amounts of CTB-specific IgA and LPS-specific IgA, IgG, and IgM Ab were found in rectal secretions of Rimm women. These data suggest that in women, Nimm alone could induce specific Ab in serum, the genital tract, and rectum. However, induction of genital tract and rectal Ab responses of the magnitude generated by local V-FPimm or Rimm will likely require administration of comparably high nasal vaccine dosages.

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“…Os efeitos de progesterona (acetato de medroxiprogesterona) em dose única foram estudados em 20 mulheres com biópsias seriadas por 3 meses 23 . Outro estudo avaliou os efeitos do mesmo hormônio em uso prolongado de 2 a 3 anos em 20 usuárias e 20 controles 24 .…”

Section: Resultsunclassified

Influência do uso de anticoncepcionais hormonais orais sobre o número de células de Langerhans em mulheres com captura híbrida negativa para papilomavírus humano

Uchimura¹,

Ribalta²,

Focchi³

et al. 2005

Rev. Bras. Ginecol. Obstet.

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726Rev Bras Ginecol Obstet. 2005; 27(12): 726-30 RESUMO Objetivo: estudar a influência do uso de anticoncepcionais orais (AO) sobre o número de células de Langerhans em mulheres sem infecção cervical por papilomavírus humano (HPV). Métodos: foram incluídas trinta mulheres com alterações citológicas e biópsia dirigida pela colposcopia com amostras de colo uterino sem sinais de infecção por HPV. A ausência de DNA de HPV foi confirmada pela captura híbrida. As células de Langerhans foram identificadas pela reação de imunohistoquímica com uso de antígenos anti-S100. As células visualizadas em microscopia de luz foram contadas utilizando o software Cytoviewer. Para análise estatística utilizou-se o teste não paramétrico de soma das ordens de Wilcoxon. Resultados: a média do número de células de Langerhans em mulheres usuárias de AO foi de 320,7/mm 2 e em não usuárias 190,7/mm 2 , não sendo esta diferença significante. Na camada intermediária do epitélio cervical observou-se tendência ao aumento dessas células, com as médias 192,1/mm 2 para usuárias e 93,4/mm 2 para não usuárias de AO (p=0,05). Conclusões: no presente estudo não se observou diferença significativa no total de células de Langerhans entre as usuárias e não usuárias de AO, porém, na camada intermediária do epitélio observou-se tendência ao aumento no número dessas células entre as usuárias de AO. Este resultado sugere que os AO podem induzir alterações no número das células de Langerhans, considerando porém o limitado número de casos, este achado não pode ser confirmado. PALAVRAS-CHAVE:Células de Langerhans; Anticoncepcionais orais; Hormônios; Imunohistoquímica; Infecções por Papillomavirus ABSTRACT Purpose: to study the influence of the use of oral contraceptives (OC) on the number of Langerhans' cells in women without cervical infection by human papillomavirus (HPV). Methods: thirty women who presented abnormal cervical cytology and colposcopy-guided biopsy with samples of uterine cervix negative for HPV were selected. The absence of HPV DNA was confirmed by hybrid capture. Langerhans' cells were identified by immunohistochemistry using anti-S100 antigens. The cells visualized in light microscopy were counted using the Cytoviewer software. The nonparametric Wilcoxon rank sum test was employed for statistical analysis. Results: the average number of Langerhans' cells in OC users was 320.7/mm 2 and in non-users 190.7/mm 2 , this difference being statistically nonsignificant. In the intermediary layer of the cervical epithelium a tendency towards the increase of these cells was observed, with the averages 192.1/mm 2 for OC users and 93.4/mm 2 for non-users (p=0.05). Conclusions: the present study reports a tendency towards the increase in the number of the Langerhans' cells among OC users. This result suggests the OC may induce alterations in the number of Langerhans' cells, but considering the limited number of cases, more studies should be developed for a definitive conclusion.

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The effect of one injection of Depo-Provera® on the human vaginal epithelium and cervical ectopy

Cited by 112 publications

References 13 publications

Anatomic and Hormonal Changes in the Female Reproductive Tract Immune Environment during the Life Cycle: Implications for HIV/STI Prevention Research

Anatomic and Hormonal Changes in the Female Reproductive Tract Immune Environment during the Life Cycle: Implications for HIV/STI Prevention Research

Differential Induction of Mucosal and Systemic Antibody Responses in Women After Nasal, Rectal, or Vaginal Immunization: Influence of the Menstrual Cycle

Influência do uso de anticoncepcionais hormonais orais sobre o número de células de Langerhans em mulheres com captura híbrida negativa para papilomavírus humano

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