The effect of Mycoplasma bovis on fertilization processes in vitro with bull spermatozoa and zona-free hamster oocytes

Eaglesome, M.D.; García, Manuel

doi:10.1016/0378-1135(90)90004-f

Cited by 18 publications

(13 citation statements)

References 17 publications

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“…Furthermore, these authors did not observe any effect on sperm viability in human semen inoculated with this mycoplasma species, consistent with the effect of Ma observed here. In another study in which DS was spiked with Mb [44], another species of this cluster that does not ferment glucose [45], no apparent effect on sperm motility was detected. This suggests that the presence of sugar in contaminated sperm diluents could induce sperm damage by glucosefermenting mycoplasma species.…”

Section: Discussionmentioning

confidence: 83%

Survival capacity of Mycoplasma agalactiae and Mycoplasma mycoides subsp capri in the diluted semen of goat bucks and their effects on sperm quality

Gómez-Martín

Vieira

et al. 2015

Theriogenology

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Section: Discussionmentioning

confidence: 83%

Survival capacity of Mycoplasma agalactiae and Mycoplasma mycoides subsp capri in the diluted semen of goat bucks and their effects on sperm quality

Gómez-Martín

Vieira

et al. 2015

Theriogenology

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“…Reproductive tract problems have been noted in both male and female animals following experimental infection with M. bovis or M. bovis ‐containing semen (Wrathall, Ayling, & Simmons, ) indicating that reduced reproductive performance may result from natural M. bovis infection. Eaglesome and Garcia () found that high levels of M. bovis significantly reduced the ability of bovine sperm to penetrate and form pronuclei in hamster oocytes, leading to the suggestion that use of infected semen might adversely affect the fertilization process in cattle. Related to parturition, a recent publication raised awareness of the ability of M. bovis to cause infected seromas in multiple animals following Caesarean section.…”

Section: The Causative Organismmentioning

confidence: 99%

Gap analysis ofMycoplasma bovisdisease, diagnosis and control: An aid to identify future development requirements

Calcutt

Lysnyansky

Sachse

et al. 2018

Transbound Emerg Dis

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There is a worldwide problem of disease caused by Mycoplasma (M.) bovis in cattle; it has a significant detrimental economic and animal welfare impact on cattle rearing. Infection can manifest as a plethora of clinical signs including mastitis, pneumonia, arthritis, keratoconjunctivitis, otitis media and genital disorders that may result in infertility and abortion. Current diagnosis and control information are reviewed and analysed to identify gaps in knowledge of the causative organism in respect of the disease pathology, diagnosis and control methods. The main considerations are as follows: no vaccines are commercially available; antimicrobial resistance is increasing; diagnostic and antimicrobial sensitivity testing needs to be improved; and a pen-side test would facilitate more rapid diagnosis and implementation of treatment with antimicrobials. More data on host susceptibility, stress factors, immune response and infectious dose levels are required. The impact of asymptomatic carriers, M. bovis survival in the environment and the role of wildlife in transmitting the disease also needs investigation. To facilitate development of vaccines, further analysis of more M. bovis genomes, its pathogenic mechanisms, including variable surface proteins, is required, along with reproducible disease models.

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“…Many investigators have reported modifications of the sperm penetration assay protocol using zona-free hamster ova (SPA) to evaluate sperm fertility. A relatively common assay used for fertility determination in many laboratories is a xenogeneic sperm penetration test using human (Sofikitis et al, 2000), bovine (Eaglesome & Garcia, 1990) or rabbit (Rajeev & Reddy, 2004) spermatozoa and hamster oocytes. While the SPA has received widespread attention as a test of sperm fertility, the indefinite range of penetration levels that constitute normal fertility, interassay variability and lack of quality control are inherent problems of this bioassay system.…”

Section: Discussionmentioning

confidence: 99%

The sperm penetration assay predicts the litter size in pigs

You

Park

et al. 2010

Int J of Andrology

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The prediction of sperm fertility is of paramount importance for breeding animals. Multiple laboratory approaches have been developed for this purpose, but they have yielded equivocal results. The objective of this study was to develop and standardize to a method for predicting fertility in vivo in boars using the in vitro penetration assay. To increase the sensitivity and reduce false-negative results of the assay, each step in the procedure was standardized and quality control was applied. Maximum penetration of hamster zona-free oocytes and immature porcine oocytes was obtained using heparin-treated sperm cells. Hamster zona-free oocytes showed a significantly higher penetration than immature porcine oocytes. To eliminate interassay variability, two frozen bull semen samples were applied. All possible variables related to the female were excluded. The SPA (sperm penetration assay using zona-free oocytes) result showed significant correlation with historic average litter size but had no significant correlation with farrowing rates. To determine the normal range for the SPA, lower limits of the sperm fertility index were established as 1.2 for the small litter sizes (<8 piglets) and 2.5 for the large litter sizes. The overall accuracy was 92 and 96% respectively, for the small and large litter sizes. Our laboratory has standardized the procedure for the SPA, resulting in greatly increased sensitivities for small and large litter sizes. The protocol increases the ability to discriminate between good and poor fertility groups and it was highly effective at ranking 24 boars by litter size into large and small litter groups.

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The effect of Mycoplasma bovis on fertilization processes in vitro with bull spermatozoa and zona-free hamster oocytes

Cited by 18 publications

References 17 publications

Survival capacity of Mycoplasma agalactiae and Mycoplasma mycoides subsp capri in the diluted semen of goat bucks and their effects on sperm quality

Survival capacity of Mycoplasma agalactiae and Mycoplasma mycoides subsp capri in the diluted semen of goat bucks and their effects on sperm quality

Gap analysis ofMycoplasma bovisdisease, diagnosis and control: An aid to identify future development requirements

The sperm penetration assay predicts the litter size in pigs

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