The effect of intratympanic vitamin C administration on cisplatin-induced ototoxicity

Abstract

The objective of this study is to investigate the effect of intratympanic injection of vitamin C on cisplatin-induced ototoxicity. The study included 24 albino adult female rats (48 ears). The study animals were divided into four groups each of which was composed of six animals including a control (intraperitoneal cisplatin), a cisplatin-saline (saline intratympanic + intraperitoneal cisplatin), a C vit (intratympanic vitamin C) and a cisplatin-C vit group (intraperitoneal cisplatin + intratympanic vitamin C). As two animals had died due to cisplatin-induced ototoxicity (one in the control and one in the cisplatin-saline group) they were excluded from the study. The experiment was terminated, performing distortion product otoacoustic emission (DPOAE) measurement prior to procedures and at the end of the experiment. The results of the statistical analysis were evaluated. In the cisplatin-C vit group, there were no significant decreases in DPOAE amplitudes at 2 kHz (p > 0.05). Although a decrease was observed in DPOAE amplitudes at 2.8, 4, 6, and 8 kHz frequencies, these amplitude reductions were significantly lower than the control group (p < 0.05). Intratympanic vit C infusion provided a protective effect against cisplatin-induced ototoxicity primarily at 2 kHz and at other frequencies (2.8, 4, 6, and 8 kHz), and it did not produce a toxic effect in the cochlea.

The Protective Effect of Intratympanic Alpha Lipoic Acid on Cisplatin-Induced Ototoxicity on Rats

“…The protective effect of intratympanic vitamin C, an antioxidant used in cisplatin-induced ototoxicity, was evaluated at 20 mg/kg administration by DPOAE in the range of 2-8 kHz. In this study, a higher protective effect was demonstrated at 2 kHz than at other frequencies [26] . In another study in rats, the otoprotective effect of intratympanic vitamin E in cisplatin ototoxicity was evaluated in the range of 4-16 kHz by ABR.…”

“…In recent years, many chemoprotective and antioxidant agents have been studied experimentally in order to antagonize the toxic effects of cisplatin on the cochlea, and their otoprotective effects have been investigated [6,7,18,[23][24][25][26][27][28] . Intratympanic administration of such agents, including α-LA, is applied today more as a rational method [6,8,26,29] .…”

“…Intratympanic administration of such agents, including α-LA, is applied today more as a rational method [6,8,26,29] . Table 3.…”

“…Resveratrol (grapes), vitamin C (citrus fruits, kiwi, melon, etc. ), and lycopene (tomatoes, grapefruit), which are present in foods that we consume daily, are antioxidants used for this purpose [2,3,26] . α-LA, which we used in our study, is a natural antioxidant found in broccoli, spinach, tomatoes, and kidney, liver, and heart tissue from animal products [11] .…”

The Protective Role of Molsidomine on the Cisplatin-Induced Ototoxicity

“…Many antioxidant agents have been studied to prevent CIO [6][7][8][9][10]. MOL is a prodrug, belonging to the class of sydnonimines, has been used widely as an antianginal agent as a vasodilator.…”