The effect of genistein on lipid levels and LDLR, LXRα and ABCG1 expression in postmenopausal women with hyperlipidemia

Abstract

BackgroundThis study investigates the effect of genistein (Gen) on the lipid profiles and expression of low-density lipoprotein receptor (LDLR), liver X receptor α (LXRα) and ATP-binding cassette transporter G1 (ABCG1) in the plasma macrophages of postmenopausal women with hyperlipidemia in China.MethodsThis study considered 187 cases, where 160 postmenopausal women had hyperlipidemia. The subjects were divided into placebo group (PG) and experimental group (EG). EG received 60 mg/day of Gen, PG received placebo for 6 months. Body weight, height, waist circumference, body mass index and glucose levels were determined according to standard operating procedures. The triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoprotein-A1 (Apo-A1) and apolipoprotein-B (Apo-B) levels were detected in the plasma macrophages using ELISA. The protein and mRNA expression levels of LDLR, LXRα and ABCG1 were detected by western blot and real-time PCR techniques, respectively.ResultsCompared to the baseline, Gen effectively lowered TG, TC and LDL-C levels, whereas HDL-C levels as well as the protein and mRNA expression levels of LDLR, LXRα and ABCG1 (p < 0.05) were increased. There was a significant difference in the expression of LDLR protein between the two groups (p < 0.05). The mRNA expression levels of LDLR, LXRα and ABCG1 were significantly increased in the EG compared to the PG.ConclusionGen effectively modulated the plasma lipid indices. The cholesterol-lowering effects of Gen may be attributed to its regulation on some of the key genes involved in cholesterol homeostasis.

On the Hypolipidemic Activity of Elicited Soybeans: Evidences Based on Computational Analysis

“…Those three isoflavonoids were the possible compound to have hypolipidemic activity through modulating de novo cholesterol biosynthesis. These results supported previous studies which reported that Daidzein, Genistein, and Glycitein could repair blood lipid profile [40][41][42][43] and suggested a new mechanism of hypocholesterolemic activity through the modulation of cholesterol biosynthesis. The inhibition of HMGCR as the rate-limiting factor of cholesterol biosynthesis upregulates LDL Receptor (LDLR) and allows plasma cholesterol uptake to achieve lower blood cholesterol levels [44].…”

The Hypolipidemic Effect of Dalbergia odorifera T. C. Chen Leaf Extract on Hyperlipidemic Rats and Its Mechanism Investigation Based on Network Pharmacology

“…Additionally, genistein and biochanin A are the main components of D. odorifera leaves [ 27 ]. In the previous reports, genistein had a hypolipidemic effect on menopausal women with hyperlipidemia [ 43 ] and ameliorated dyslipidemia and hepatic steatosis in hyperlipidemic hmsters [ 32 ] and diabetic mice [ 44 ]. Biochanin A mitigates dyslipidemia in hyperlipidemic mice [ 33 ], atherosclerotic mice [ 45 ], and diabetic rats [ 46 ].…”

Physicochemical properties, metabolomic analysis, antioxidant and lipid-lowering activity of a functional beverage based on cocona (Solanum sessiliflorum Dunal)

“…Peaks 23 and 25 with pseudomolecular ions at m/z: 269.0595 and 431.09843 were devoided as genistein ( C 15 H 10 O 5) and genistein 5-O-glucoside ( C 21 H 20 O 10). Genistein showed lipid lowering effects modulating the plasma lipid indices in women with hyperlipidemias (Zhang & Chi, 2019).…”