The effect of fucoidan from brown seaweed Sargassum wightii on WSSV resistance and immune activity in shrimp Penaeus monodon (Fab)

Immanuel, G.; Sivagnanavelmurugan, Madasamy; Marudhupandi, Thangapandi; Radhakrishnan, Srinivasan; Palavesam, A.

doi:10.1016/j.fsi.2012.01.003

Cited by 150 publications

(75 citation statements)

References 69 publications

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“…1). Immanuel et al (2012) showed similar result where the proPO activity in Penaeus monodon fed fucoidan extracts of Sargassum wightii were decreased after challenge test with WSSV and also positively correlated with decreasing of the hemocytes. J.…”

Section: Discussionsupporting

confidence: 58%

Dietary Synbiotic Microcapsule Influence the Immune Responses, Growth Performance and Microbial Populations to White Spot Syndrome Virus in Pacific White Shrimp (Litopenaeus vannamei)

Febrianti¹,

Yuhana²,

Widanarni³

2015

J. of Fisheries and Aquatic Science

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White Spot Syndrome Virus (WSSV) is one of the most devastating shrimp pathogen which causes up to 70-90% mortality in commercial shrimp farming. This study was aimed to evaluate the effectiveness of synbiotic microcapsule (Bacillus NP5 Rf R and mannan oligosaccharide combination) feed supplementation on the immune responses and growth performance of WSSV infected Pacific white shrimp. Shrimps (4.411±0.395 g) were cultured and fed by pellet containing synbiotic microcapsule at different dosages for 30 days, i.e., designated as 0.5% (M1), 1% (M2), 2% (M3) (w/w) and without synbiotic microcapsule addition (negative and positive controls). The synbiotic was encapsulated by spray drying method. Challenge test was performed at 31st of cultivation day by performing intramuscular injection of WSSV filtrate (10 4 copies mLG 1 ). Compared with control, the shrimp fed by M2 and M3 were found to have higher (p<0.05) value of Total Hemocyte Count (THC), pro Phenol Oxidase (proPO) and Respiratory Burst (RB) activity compared to positive control. The results showed that the Specific Growth Rate (SGR) of M1 and M2 were higher (p<0.05) than controls. Yet, the Feed Conversion Ratio (FCR) were lower (p<0.05) than controls. Above all, the survival rate of shrimps fed with microcapsule synbiotic were higher (p<0.05) than controls.

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Section: Discussionsupporting

confidence: 58%

Dietary Synbiotic Microcapsule Influence the Immune Responses, Growth Performance and Microbial Populations to White Spot Syndrome Virus in Pacific White Shrimp (Litopenaeus vannamei)

Febrianti¹,

Yuhana²,

Widanarni³

2015

J. of Fisheries and Aquatic Science

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“…The broad band centered at 3435 cm -1 assigned to hydrogen bonded O-H stretching vibration. [28][29][30] The band at 2939 cm -1 was attributed the C-H stretching pyranoid ring and C6 of fucose and/or galactose units. 13,30 The band at 1614 cm -1 indicated the stretching of asymmetric carboxylate O-C-O vibration.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity of Fucoidan from Three Tropical Brown Algae Against Breast and Colon Cancer Cell Lines

Isnansetyo¹,

Lutfia²,

Nursid³

et al. 2016

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Introduction:Fucoidan is a sulfated polysaccharide that has a wide range of bioactivities including anti-cancer. This polysaccharide commonly is extracted from marine brown seaweed. There is lack of information on the fucoidan extracted from tropical brown algae and its anti-cancer activity. Objectives: The objectives of this study were to purify fucoidan from Sargassum sp., Turbinaria sp. and Padina sp., and to evaluate their cytotoxicity against breast cancer (MCF-7) and colon cancer cells (WiDr). Materials and Methods: Fucoidan extraction was conducted by using acid extraction method. Purified fucoidans were obtained by DEAE cellulose column chromatography and confirmed by HPLC and FT-IR spectrometry. The cytotoxicity was evaluated by using the MTT (3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide) assay. Results: Fucoidan from Sargassum sp. and Turbinaria sp. showed low cytotoxicity with IC 50 ranging between 461-663 µg/mL. Higher cytotoxicity against MCF-7 and WiDr was showed by fucoidan from Padina sp. with IC 50 of 144 and 118 µg/mL, respectively. While its IC 50 against Vero cells was 501 µg/mL.Standard fucoidan from Fucus vesiculosus exhibited IC 50 of 60, 63 and 211 µg/mL against MCF-7, WiDr and Vero Cells, respectively. Although the IC 50 was higher than that of standard fucoidan, Padina sp. fucoidan showed cytotoxicity comparable with standard fucoidan at concentrations below 100 µg/mL. Conclusion: These results indicated that Padina sp.fucoidan showed potential selective cytotoxicity, and promising for the development of an anti-cancer compound.

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“…Dietary polysaccharides, in particular alginates (Cheng et al 2004) and fucoidans (Chotigeat et al 2004, Immanuel et al 2012b derived from seaweeds, have also shown promise in protecting shrimp from infectious diseases.…”

Section: Discussionmentioning

confidence: 99%

“…With regard to total carbohydrate (46.1%) and fucose (29.2%) contents determined for the S. wightii alginate, these varied considerably from those identified in alginates extracted from various algae harvested from the Egyptian Red Sea coast including Cystoseira trinode (74.9 and 11.6%, respectively), S. dentifolium (57.9 and 5.6%), S. asperifolium (32.2 and 4.2%) and S. latifolium (42.3 and 8.2%) (Larsen et al 2003). The variations in biochemical compositions of seaweed polysaccharides mainly depend on the seaweed species, anatomical regions, growth con ditions, extraction procedures and analytical methods (Immanuel et al 2012b). …”

Section: Discussionmentioning

confidence: 99%

“…Immanuel et al (2012b) stated that the dietary administration of fucoidan of Sargassum wightii increased resistance against WSSV in P. monodon, and observed that the percent mortality of experimental groups (0.1 to 0.3% concentration of fucoidan) of shrimp was reduced by 50.81 to 68.06% over the control group. Immanuel et al (2010) also reported the effect of hot water extracts of S. wightii and S. duplicatum on the reduction in mortality in P. monodon PL against WSSV.…”

Section: Discussionmentioning

confidence: 99%

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Sodium alginate from Sargassum wightii retards mortalities in Penaeus monodon postlarvae challenged with white spot syndrome virus

Immanuel

Sivagnanavelmurugan²,

Balasubramanian³

et al. 2012

Dis. Aquat. Org.

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Sodium alginate extracted from brown seaweed Sargassum wightii (16.35 ± 1.42%, mean [± SD] yield from 5 extractions) was prepared as a powder or beads and used to enrich Artemia nauplii at concentrations of 100, 200, 300 and 400 mg l −1 . The alginate-enriched nauplii were fed to Penaeus monodon shrimp postlarvae (PL) stage 15 (PL15, i.e. 15 d old) for 20 d. Mean weight gain and specific growth rate over this period were 0.24 g and 15.8%, respectively, in PL groups not fed alginate, and 0.20−0.28 g and 14.7−16.5%, respectively, in PL groups fed alginate. Amongst PL35 then challenged with white spot syndrome virus (WSSV) by immersion, all PL not fed alginate died within 9 d. However, amongst PL fed the 4 concentrations of alginate powder or beads, mortality rates reduced with increasing alginate concentration, and between 25 and 32% PL remained alive when the bioassay was terminated on Day 21. Amongst alginate-fed PL groups compared with the control group, mortality was reduced by 26.5 to 58.4%. Nested PCR detection of WSSV revealed sodium alginate concentration-dependent reductions in infection loads. The data indicate that sodium alginate extracted from brown seaweed and fed to P. monodon can retard progression of WSSV disease.

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The effect of fucoidan from brown seaweed Sargassum wightii on WSSV resistance and immune activity in shrimp Penaeus monodon (Fab)

Cited by 150 publications

References 69 publications

Dietary Synbiotic Microcapsule Influence the Immune Responses, Growth Performance and Microbial Populations to White Spot Syndrome Virus in Pacific White Shrimp (Litopenaeus vannamei)

Dietary Synbiotic Microcapsule Influence the Immune Responses, Growth Performance and Microbial Populations to White Spot Syndrome Virus in Pacific White Shrimp (Litopenaeus vannamei)

Cytotoxicity of Fucoidan from Three Tropical Brown Algae Against Breast and Colon Cancer Cell Lines

Sodium alginate from Sargassum wightii retards mortalities in Penaeus monodon postlarvae challenged with white spot syndrome virus

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