The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

Abstract

The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes' oxygentransport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen-air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl 2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca 2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids' composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.

Investigating lipid headgroup composition within epithelial membranes: a systematic review

“…The search terms comprised of two pairs (a) membrane composition OR phospholipid composition AND (b) human erythrocyte OR human red blood cell; each pair being combined using a Boolean OR operator, and then a total AND combinator to yield 23 results, of which only 9 were appropriate for inclusion. [128][129][130][131][132][133][134][135][136] One important note on the erythrocyte data; only 2 of the 9 compositions contained data that related to cholesterol content, and as such it may not be completely representative. However, where cholesterol contents were reported, the amounts of other lipids were reduced in all cases compared to where they were not reported.…”

Changes in the oxygen transport system of erythrocytes in testing the general endurance of students

“…Other studies determine: • The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin [9];…”

Effect of Rat Exposure to Moderate Pressure Oxygen Atmosphere on Resistance of Erythrocytes against Hemolysis

“…It should be stressed that the erythrocytic part of the blood system is the first to respond to activation of free radical oxidation and the first to exhaust their compensatory function [9]. Hyperoxia in vitro upregulates LPO in erythrocyte membranes and modifies lipid composition and functional parameters of erythrocytes -first of all, the oxygen transporting properties of hemoglobin [15].…”