The effect of diluting crystallization droplets on protein crystallization in vapor diffusion method

Lu, Qi; Yin, Da‐Chuan; Xie, Shengshi; Liu, Yong-Ming; Chen, Rui‐Qing

doi:10.1002/crat.201100178

Cited by 4 publications

(2 citation statements)

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“…In a recent study, the effects of diluting either protein or crystallization agents in the droplets on reproducibility and screening efficiency of protein crystallization was investigated [80]. The results indicate that reducing the initial concentration of the crystallization agent in the droplet leaving the protein concentration unchanged increases the rate of vapor diffusion and produces higher success rate of protein crystallization.…”

Section: Conventional Crystallization Methodsmentioning

confidence: 99%

An Overview of Biological Macromolecule Crystallization

Krauss

Merlino

Vergara

et al. 2013

IJMS

123

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The elucidation of the three dimensional structure of biological macromolecules has provided an important contribution to our current understanding of many basic mechanisms involved in life processes. This enormous impact largely results from the ability of X-ray crystallography to provide accurate structural details at atomic resolution that are a prerequisite for a deeper insight on the way in which bio-macromolecules interact with each other to build up supramolecular nano-machines capable of performing specialized biological functions. With the advent of high-energy synchrotron sources and the development of sophisticated software to solve X-ray and neutron crystal structures of large molecules, the crystallization step has become even more the bottleneck of a successful structure determination. This review introduces the general aspects of protein crystallization, summarizes conventional and innovative crystallization methods and focuses on the new strategies utilized to improve the success rate of experiments and increase crystal diffraction quality.

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Section: Conventional Crystallization Methodsmentioning

confidence: 99%

An Overview of Biological Macromolecule Crystallization

Krauss

Merlino

Vergara

et al. 2013

IJMS

123

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“…In this study, a membrane crystallization method was investigated for lysozyme, which can obtain crystals in a shorter time than the conventional protein crystallization method [7][8][9]. In this method, lysozyme solution is placed on an ultrafiltration membrane, and pressure is applied from above using nitrogen gas to concentrate the lysozyme and crystallize it.…”

Section: Introductionmentioning

confidence: 99%

Estimation of Lysozyme Concentration on a Membrane Surface Using a Membrane Crystallization Method

2023

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High‐quality single crystals are necessary for structural analysis of proteins. However, it is difficult to obtain high‐quality single crystals in a short time. Therefore, a membrane crystallization method was applied in which lysozyme is concentrated on the membrane surface using an ultrafiltration membrane. Membrane surface concentrations, which cannot be measured, were calculated based on the measurable permeation flux. At all operating pressures, the measured and simulated permeation fluxes were in close agreement, allowing the membrane surface concentration to be estimated. The membrane surface concentration increased rapidly at all pressures during the initial pressurization, indicating that crystals were formed at an early stage.

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