Estimation of Lysozyme Concentration on a Membrane Surface Using a Membrane Crystallization Method

Abstract

High‐quality single crystals are necessary for structural analysis of proteins. However, it is difficult to obtain high‐quality single crystals in a short time. Therefore, a membrane crystallization method was applied in which lysozyme is concentrated on the membrane surface using an ultrafiltration membrane. Membrane surface concentrations, which cannot be measured, were calculated based on the measurable permeation flux. At all operating pressures, the measured and simulated permeation fluxes were in close agreement, allowing the membrane surface concentration to be estimated. The membrane surface concentration increased rapidly at all pressures during the initial pressurization, indicating that crystals were formed at an early stage.