The Effect of Different Forms of Sunflower Oil and Protein Sources in the Diet on Pancreatic Juice Secretion and Pancreatic Enzymes Activity in Sheep / Wpływ formy oleju słonecznikowego oraz źródła białka w dawce na sekrecję soku trzustkowego oraz aktywność enzymów trzustkowych u owiec

Abstract

The aim of the study was to determine the effect of various forms of sunflower oil in the diet with protein degraded at different rates in the rumen on pancreatic juice secretion and activity. The experiment was conducted on 24 adult Corriedale rams weighing about 40 ± 1.5 kg, catheterized in the pancreatic and bile ducts and fistulated in the duodenum. The animals were fed diets consisting of meadow hay, potato starch, different degradable protein (casein or maize gluten, a source of zein) and different forms of sunflower oil (calcium salts, seeds and oil). It was stated that addition of various forms of fat to the diet did not significantly influence the secretion of pancreatic juice, regardless of the source of protein. However, sunflower seeds and oil used in the diet had a significant effect on bile secretion, protein content, proteolytic activity of trypsin and plasma lipid indices. No significant differences were observed in the lipolytic activity of the pancreatic juice, although lipase activity was higher when zein was used as the main protein source. It was concluded that dietary addition of certain combinations of protected or unprotected sunflower oil and different degradable protein may improve pancreatic activity and probably affect plasma lipid indices in sheep.