The Effect of Concentrated Growth Factors in the Treatment of Periodontal Intrabony Defects

Qiao, Jing; Duan, Jinyu; Zhang, Yong; Chu, Yi; Sun, Changzhou

doi:10.4155/fsoa-2016-0019

Cited by 42 publications

(47 citation statements)

References 35 publications

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“…However, periodontal tissue destruction and alveolar bone defects significantly weaken the stability and shorten the service life of dental implants (2). To improve the success rate of dental implants, concentrated growth factor (CGF) can be used to promote periodontal tissue regeneration and osteogenic differentiation (3). CGF is a gel-like substance, obtained by centrifugation of venous blood, which is rich in growth factors and fibrin (4–8), and CGF combined with bone graft material promotes immediate periodontal tissue regeneration and osteogenic differentiation (8,9).…”

Section: Introductionmentioning

confidence: 99%

Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF‑α

Yang

Zhang

et al. 2018

Mol Med Report

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The purpose of this study was to evaluate the effects of concentrated growth factor exudate (CGFe) on human periodontal ligament cells (hPDLCs) stimulated by tumor necrosis factor (TNF)-α. From the peripheral blood of healthy donors, CGFe was prepared according to the Sacco protocol after 7 days of incubation. The hPDLCs were cultured by a tissue explant method and identified with anti-vimentin and anti-cytokeratin antibodies. Cells were subjected to four different treatments: i) Control; ii) TNF-α (10 ng/ml); iii) CGFe (concentration 50%); and iv) CGFe+TNF-α. The proliferation of hPDLCs was measured with Cell Counting Kit-8 assays. Osteogenic differentiation and mineralization were determined by Alizarin Red S staining, alkaline phosphatase activity, western blotting and reverse transcription-quantitative polymerase chain reaction. CGFe enhanced cell proliferation and upregulated ALP activity, the mineralization level, and osteogenic-associated osteocalcin, runt-related transcription factor 2 and Osterix gene expression in hPDLCs under inflammatory conditions induced by TNF-α. The present study demonstrated that CGFe enhanced hPDLC proliferation and osteogenic differentiation in the presence of TNF-α-induced inflammation. As CGFe can be obtained from the venous blood of patients, it generates no immune reaction. Thus, it is more economical and beneficial to use CGFe in clinical periodontal regeneration practice than synthetic growth factors.

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Section: Introductionmentioning

confidence: 99%

Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF‑α

Yang

Zhang

et al. 2018

Mol Med Report

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“…As seen in the result of this review, CGF was used alone in in vitro studies, but in in vivo studies, the combination of CGF with stem cells or biomaterials has been reported (Chen et al, ; Durmuslar et al, ; Masuki et al, ; Qiao et al, ; Qiao & An, ; Rodella et al, ). Tissue engineering is a complex of stem cells, scaffold, and growth factors.…”

Section: Discussionmentioning

confidence: 99%

“…For this purpose, fresh CGF will be cut into small fragments and mixed with biomaterial at a defined volume ratio. The result of the mechanical mixture is an adhesive paste that can be placed in tissue defect (Qiao, Duan, Zhang, Chu, & Sun, 2016).…”

Section: Methods Of Cgf Preparation and Applicationmentioning

confidence: 99%

“…Adult cells or cell lines used for assessment of CGF are NHDF, HUVEC, HOB, Rat bone marrow cells, Schwann cells, SaOS-2, periosteal cells, and dental pulp cells (Table 3) Most studies stated improvement of tissue healing or regeneration in the presence of CGF, yet three studies investigating its effect on periimplantitis, implant stability, and immediate implants reported no significant difference or lower effect of CGF (Isler et al, 2018;Özveri Koyuncu, _ Içpınar Çelik, et al, 2019;Yang et al, 2015). Combination of CGF with stem cells or grafts resulted in better results than CGF alone Durmuslar et al, 2016;Honda et al, 2013;Kizilaslan et al, 2020;Qiao et al, 2016;Wang et al, 2017). Three studies compared CGF to other platelets concentrates; one study reported no significant difference between CGF, PRP, and PRF on bone formation in rabbit-skull defect (Kim et al, 2014), another study also confirmed no significant difference in GTR between A-PRF and CGF (Lei et al, 2019); while Park et al indicated better bone formation of CGF than PRF in femur defect of adult dogs (Park et al, 2016).…”

Section: Effect Of Cgf On Adult Cells or Cell Linementioning

confidence: 99%

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In vitro and in vivo effects of concentrated growth factor on cells and tissues

Tabatabaei

Aghamohammadi

Tayebi

2020

J Biomedical Materials Res

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This article reviews the biological outcome of the concentrated growth factor (CGF), a new platelet derivative used for tissue regeneration, in published articles related to the use of this product in basic and clinical studies. An electronic literature research using PubMed and SCOPUS was performed using combination of keywords: “concentrated growth factor” (OR “CGF”), AND “stem cells,” AND “cells” OR “cell proliferation” OR “cell migration” OR “cell differentiation,” AND “repair” OR “survival” OR “revitalization,” AND “tissue” OR “bone.” Forty‐five articles that were published between 2012 and 2020 met the inclusion criteria. These studies have used CGF as fresh solid form, freeze‐dried, membrane, extract, or exudate. Most studies demonstrate the positive effects of CGF in a dose‐dependent manner under certain concentrations. Studies comparing CGF with other platelet concentrates, report lower efficiency, no statistically significant differences, or better results for CGF. Combination of CGF with stem cells and biomaterials significantly improves bone regeneration and the effect of allograft or collagen membrane is better than CGF alone. For a better examination of the biological outcomes of CGF, the standardization of CGF preparation regarding the choice of the test tube material for blood collection, the required volume of blood, the necessary count of platelets in CGF, and the most appropriate type of CGF are recommended.

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“…The result of his study reveals that the MPM specimen presented with a mass of cancellous bone with areas of diverse degree of maturation, this is because osteoblastic activity of the specimen is more prominent and active, resulting in the deposition of woven bone while the PRF specimen presented with a less osteoblastic activity, resulting in a fibrotic bone marrow with little vascularity. Also, we reviewed a study that was reported in 2016 by Qiao et al [28], who showed the effect of concentrated growth factors on the treatment of grade II furcation involvements in comparative study, where the subjects were randomly divided into two groups; the furcation involvements in the experimental group were treated with bone graft therapy combined with CGFs, and the furcation involvements in the control group were treated with bone graft therapy alone. The changes of clinical and CBCT data at baseline and 1 year post-surgery were compared between the two groups and results showed positive role of CGF in the treatment of grade II furcation involvements other than the control group.…”

Section: Discussionmentioning

confidence: 99%

The effectiveness of platelet concentrations in periodontal surgeries

Al-Azem

Ali

Mostafa

2018

IJDR

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Platelets release several growth factors which stimulate tissue regeneration. Several techniques for platelet concentrates such as platelet rich plasma (PRP), plasma rich in growth factors (PRGF), platelet rich fibrin (PRF) and concentrated growth factors (CGF) have been introduced in dental surgeries for the prevention of hemorrhage and acceleration of tissue regeneration. However, a fabricating growth factors-enriched bone graft matrix which is called “sticky bone” has been demonstrated to provide stabilization in bony defects. In this article, we presented the method of preparing and utilizing CGF and sticky bone and evaluate the effect of CGF mixed with bone graft and CGF barrier membrane in periodontal surgeries.Methodology: We used websites such as PubMed, Scopus, and ISI Web of knowledge to get related articles about this subject. The research process involved specific key words " concentrated growth factor”- “Platelet rich fibrin”- growth factors-enriched bone graft” - “sticky bone” to find more articles which published from 2007 to March 2018.Results: We reviewed 48 articles, 43 articles were excluded. Only five articles have been conducted. Original human studies and case report were included.Conclusion: We concluded that the use of sticky bone and CGF is effective in bone grafting and implant.

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The Effect of Concentrated Growth Factors in the Treatment of Periodontal Intrabony Defects

Cited by 42 publications

References 35 publications

Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF‑α

Concentrated growth factor exudate enhances the proliferation of human periodontal ligament cells in the presence of TNF‑α

In vitro and in vivo effects of concentrated growth factor on cells and tissues

The effectiveness of platelet concentrations in periodontal surgeries

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