The Effect of Autologous Platelet Lysate Eye Drops: An In Vivo Confocal Microscopy Study

Abstract

Purpose. To determine the effectiveness of autologous platelet lysate (APL) eye drops in patients with primary Sjögren syndrome (SS) dry eye, refractory to standard therapy, in comparison with patients treated with artificial tears. We focused on the effect of APL on cornea morphology with the in vivo confocal microscopy (IVCM). Methods. Patients were assigned to two groups: group A used autologous platelet lysate QID, and group B used preservative-free artificial tears QID, for 90 days. Ophthalmological assessments included ocular surface disease index (OSDI), best corrected visual acuity (BCVA), Schirmer test, fluorescein score, and breakup time (BUT). A subgroup of patients in group A underwent IVCM: corneal basal epithelium, subbasal nerves, Langerhans cells, anterior stroma activated keratocytes, and reflectivity were evaluated. Results. 60 eyes of 30 patients were enrolled; in group A (n = 20 patients) mean OSDI, fluorescein score, and BUT showed significant improvement compared with group B (n = 10 patients). The IVCM showed a significant increase in basal epithelium cells density and subbasal nerve plexus density and number and a decrease in Langerhans cells density (p < 0.05). Conclusion. APL was found effective in the treatment of SS dry eye. IVCM seems to be a useful tool to visualize cornea morphologic modifications.

Autologous serum eye drops for dry eye

“…We identified one potentially relevant record by searching the Science Citation Index for trials that cited included studies. Of 297 unique records identified, we excluded 291 records by screening titles and abstracts, and five upon review of full-text reports (Fea 2016; Hwang 2014; Li 2015; Mukhopadhyay 2015; NCT02752763). We included one new trial (Celebi 2014) in the update of this review.…”

“…Most excluded studies were non-randomized studies or reviews. We excluded five RCTs because investigators did not compare AS versus artificial tears or placebo (Fea 2016; Jaksche 2005; Li 2015; Noble 2004; Yoon 2007). …”

PL/Vancomycin/Nano-hydroxyapatite Sustained-release Material to Treat Infectious Bone Defect

“…Subsequently, the platelet membrane and other cell fragments were removed through low temperature high speed centrifugation, and the supernatant was collected, which was the PL. At last, it was preserved in the sterilized freezing tube at -80℃ [16].…”

Application of Novel Drugs for Corneal Cell Regeneration

“…PRP was shown to be effective for the improvement of both symptoms and signs of DED [ 73 ]. Plasma lysate was suggested to be helpful for the treatment of DED associated with GVHD or SS [ 74 , 75 ]. PRP was also superior to conventional treatment for the recovery of visual acuity and corneal transparency in patients with ocular chemical injury [ 76 ].…”