The effect of anthocyanin supplementation in modulating platelet function in sedentary population: a randomised, double-blind, placebo-controlled, cross-over trial

Abstract

The anti-thrombotic properties of anthocyanin (ACN) supplementation was evaluated in this randomised, double-blind, placebo (PBO) controlled, cross-over design, dietary intervention trial in sedentary population. In all, sixteen participants (three males and thirteen females) consumed ACN (320 mg/d) or PBO capsules for 28 d followed by a 2-week wash-out period. Biomarkers of thrombogenesis and platelet activation induced by ADP; platelet aggregation induced by ADP, collagen and arachidonic acid; biochemical, lipid, inflammatory and coagulation profile were evaluated before and after supplementation. ACN supplementation reduced monocyte-platelet aggregate formation by 39 %; inhibited platelet endothelial cell adhesion molecule-1 expression by 14 %; reduced platelet activation-dependant conformational change and degranulation by reducing procaspase activating compound-1 (PAC-1) (↓10 %) and P-selectin expression (↓14 %), respectively; and reduced ADP-induced whole blood platelet aggregation by 29 %. Arachidonic acid and collagen-induced platelet aggregation; biochemical, lipid, inflammatory and coagulation parameters did not change post-ACN supplementation. PBO treatment did not have an effect on the parameters tested. The findings suggest that dietary ACN supplementation has the potential to alleviate biomarkers of thrombogenesis, platelet hyperactivation and hyper-aggregation in sedentary population.

Consumption of plant extract supplement reduces platelet activating factor-induced platelet aggregation and increases platelet activating factor catabolism: a randomised, double-blind and placebo-controlled trial

“…However, in vivo reports concerning the effect of natural products consumption on VCAM levels are in agreement with the results of the present study since 8 weeks’ daily consumption of resveratrol (250 mg) ( 33 ) and 4 weeks’ consumption of green tea ( 28 ) did not alter sVCAM-1 levels in healthy volunteers. Concerning sP-selectin, in contrast with the present study, it was reported that consumption of anthocyanin capsules reduced P-selectin expression measured by flow cytometer in a sedentary population ( 27 ) and 4 weeks consumption of green tea decreased sP-selectin levels in male smokers ( 28 ) . It should be mentioned that in the first study different methodology was used ( 27 ) , and in the second one ( 28 ) baseline values of P-selectin were double compared with the values of the present study.…”

“…Concerning sP-selectin, in contrast with the present study, it was reported that consumption of anthocyanin capsules reduced P-selectin expression measured by flow cytometer in a sedentary population ( 27 ) and 4 weeks consumption of green tea decreased sP-selectin levels in male smokers ( 28 ) . It should be mentioned that in the first study different methodology was used ( 27 ) , and in the second one ( 28 ) baseline values of P-selectin were double compared with the values of the present study. Focusing in clinical trials with vitamin supplementation, much higher dosages than the ones presented in this study were used, without always have a beneficial effect on the levels of the adhesion molecules in healthy populations ( 34 – 36 ) .…”

“…As was described, the volunteers had a medium adherence to the Mediterranean diet and were overweight ( 20 ) . At baseline, the levels of the soluble adhesion molecules (sVACM, sP-selectin) and IL-6 were in the physiological range for healthy subjects ( 27 , 28 ) . As far as PAF metabolism is concerned, the activities of PAF-CPT and LpPLA 2 were similar with the previous ones reported for the Greek population ( 29 ) , while no data exist for the activities of Lyso-PAF ATC and Lyso-PAF ATE that were strongly and positively correlated with the activity of PAF-CPT of the de novo pathway, indicating a parallel regulation of the two pathways.…”

Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils

“…An increasing amount of evidence supports the beneficial role of dietary anthocyanins against CVD. Findings from recent nutritional intervention studies with anthocyanin-rich foods or extracts suggest that these compounds could exert their cardioprotective effects through modulations of platelet function by reducing the platelet activation and aggregation with leukocytes [21][22][23][24]. These processes contribute to atherosclerotic plaque formation in the blood vessel wall, playing a critical role in the development of atherosclerosis, a chronic inflammatory condition that precedes CVD.…”

“…However, these cardiovascular benefits may be, at least partly, attributed to the effect of anthocyanins on platelet function. Several dietary intervention studies with anthocyanin-rich foods or extracts support this hypothesis by reporting reduced platelet granule secretion [17,18] or ex vivo agonist-induced platelet activation and aggregation [19][20][21][22][23][24] in healthy volunteers or subjects at increased CVD risk. Furthermore, decreased platelet hyperactivity was observed in a few animal studies with mice fed a high-fat diet enriched with anthocyaninbased extracts or pure compounds [25,26].…”