The effect of aloe vera on ischemia—Reperfusion injury of sciatic nerve in rats

Güven, Mustafa; Gölge, Umut Hatay; Aslan, Esra; Şehitoğlu, Müşerref Hilal; Aras, Adem Bozkurt; Akman, Tolga; Çoşar, Murat

doi:10.1016/j.biopha.2016.02.023

Cited by 22 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that aloe can alleviate neuronal damage and protect the spinal cord from ischemia-reperfusion injury (41). Aloe has an effective neuroprotective effect on sciatic nerve ischemia-reperfusion injury (42). Aloe may serve a protective role in cerebral ischemia by inhibiting neuronal apoptosis (43).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway

2019

View full text Add to dashboard Cite

Barbaloin (Bar) has a myocardial protective effect, but its mechanism of action is uncertain. The endoplasmic reticulum stress (ERS)-mediated apoptosis pathway serves an important role in the pathogenesis of myocardial ischemia-reperfusion injury (MIRI). Inhibiting ERS may significantly improve the progression of MIRI and serve a role in its prevention. Therefore, based on current knowledge of ERS-mediated cardiomyocyte apoptosis and the cardioprotective effect of Bar, the purpose of the present study was to further evaluate the myocardial protective effect and potential mechanisms of Bar pretreatment in MIRI. The present study established a MIR rat model and randomly divided these rats into four groups. Prior to myocardial ischemia, Bar (20 mg/kg) was administered to rats once daily for 1 week. Myocardial blood serum lactate dehydrogenase and creatine kinase were subsequently measured. A terminal deoxynucleotidyl transferase mediated dUTP nick end labeling assay was used to evaluate the myocardial protective effect of Bar pretreatment on MIRI. To assess whether the ERS signaling pathway was involved in the myocardial protection mechanism of Bar pretreatment, the expression levels of ERS-associated proteins, protein canopy homolog 2 (CNPY2), glucose regulatory protein 78, transcriptional activator 4, C/EBP-homologous protein (CHOP), PKR endoplasmic reticulum kinase (PERK), caspase-12 and caspase-3 were detected by western blot analysis, immunohistochemistry or reverse transcription-quantitative polymerase chain reaction. The results confirmed that Bar pretreatment significantly reduced the damage and the level of apoptosis caused by MIR. Bar pretreatment significantly inhibited the expression of ERS-associated proteins in cardiomyocytes. In addition, the immunohistochemistry results demonstrated that Bar pretreatment significantly inhibited the CNPY2-positive cell apoptosis ratio of cardiomyocytes. Therefore, the results of the current study suggested that CNPY2 is present in cardiomyocytes and participates in the development of MIRI by initiating the PERK-CHOP signaling pathway. Bar pretreatment may attenuate MIRI by inhibiting the CNPY2-PERK apoptotic pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway

2019

View full text Add to dashboard Cite

show abstract

“…Aloe species are generally used worldwide due to its anti-tumor, antiinflammatory, antioxidant, and laxative effects [14, 26]. An experimental study on rats showed that AV had a neuroprotective effect on the sciatic nerve in I/R injury [27]. To date, though many studies have used Aloe vera , there is no study that investigated the protective effect of AV on I/R injury in the liver.…”

Section: Discussionmentioning

confidence: 99%

The Hepatoprotective Effect of Aloe Vera on Ischemia-Reperfusion Injury in Rats

Şehitoğlu

Karaboğa

Kiraz

et al. 2018

North Clin Istanbul

View full text Add to dashboard Cite

OBJECTIVE:Aloe vera is known for its antioxidant properties. In this experimental study, we aimed to investigate the efficacy of Aloe vera in ischemia-reperfusion (I/R) liver injury in rats.METHODS:Male Wistar Albino rats were divided into three groups, where the sham group (n=7) underwent no medication or surgical procedures, the I/R group (n=7) was the control group that received 45 minutes of applied abdominal aorta ischemia and rats were sacrificed 24 hours after reperfusion, and the I/R+AV group (n=7) was the treatment group that was given Aloe vera (30 mg/kg) every day followed by gastric lavage for a month before applying ischemia and performing sacrifice as in the previous group. Before sacrifice, all the liver tissues were removed. Tissues were examined for histopathological investigation, iNOS immunoreactivity and tissue biochemistry, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities.RESULTS:The SOD, CAT, and GSH-Px levels of the I/R+AV group were not significantly different from the sham group (p>0.05) but were significantly higher when compared to the I/R group. MDA levels of liver tissues were significantly lower (p<0.05) in the I/R+AV group as compared to the I/R group. Disrupted hepatic cords, sinusoidal dilatation, hemorrhage, cytoplasmic vacuolization of hepatocytes, and intensive iNOS immunoreactivity were detected in the I/R group. Decreased histopathological change score and iNOS immunoreactivity score were noticed in the I/R+AV group as compared to the I/R group.CONCLUSION:It was found that Aloe vera showed a hepatoprotective effect against I/R injury. Further research is required to determine the effective dose, administration method, and effects of Aloe vera for liver transplantation.

show abstract

“…Neurotic synthesis (nNOS) has been reported in ischemic/spinal cord injury. The neuroprotective effects of Aloe vera gel on sciatic nerve damage/reperfusion were expressed by reducing malondialdehyde, ischemic fiber degeneration, NF-?B, and increasing superoxide dismutase, and according to the results, it was suggested that Aloe vera gel have neuroprotective effects through anti-inflammatory and antioxidant mechanisms (Guven et al, 2016). Studies on PC12 cells have also shown that Aloe vera has neuroprotective effects by protecting the function and structure of mitochondria, as well as inhibiting lipid peroxidation in these cells (Wang et al, 2010).…”

Section: Discussionmentioning

confidence: 94%