The Hepatoprotective Effect of Aloe Vera on Ischemia-Reperfusion Injury in Rats

Şehitoğlu, Müşerref Hilal; Karaboğa, İhsan; Kiraz, Aslı Öztürk; Kiraz, Hasan Ali

doi:10.14744/nci.2018.82957

Cited by 13 publications

(15 citation statements)

References 27 publications

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“…Our study revealed that SOD-1, SOD-2, GPX and CAT levels were decreased following ROSC in Group II compared with Group I. The levels of these antioxidant enzymes are reduced following I/R, which causes cell damage and death due to the consumption of endogenous antioxidants as a result of ROS release ( 52 ).…”

Section: Discussionmentioning

confidence: 85%

Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes

et al. 2021

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Although multi-organ dysfunction is associated with the survival rate following cardiac arrest (CA), the majority of studies to date have focused on hearts and brains, and few studies have considered renal failure. The objective of the present study, therefore, was to examine the effects of therapeutic hypothermia on the survival rate, pathophysiology and antioxidant enzymes in rat kidneys following asphyxial CA. Rats were sacrificed one day following CA. The survival rate, which was estimated using Kaplan-Meier analysis, was 42.9% one day following CA. However, hypothermia, which was induced following CA, significantly increased the survival rate (71.4%). In normothermia rats with CA, the serum blood urea nitrogen level was significantly increased one day post-CA. In addition, the serum creatinine level was significantly increased one day post-CA. However, in CA rats exposed to hypothermia, the levels of urea nitrogen and creatinine significantly decreased following CA. Histochemical staining revealed a significant temporal increase in renal injury after the normothermia group was subjected to CA. However, renal injury was significantly decreased in the hypothermia group. Immunohistochemical analysis of the kidney revealed a significant decrease in antioxidant enzymes (copper-zinc superoxide dismutase, manganese superoxide dismutase, glutathione peroxidase and catalase) with time in the normothermia group. However, in the hypothermia group, these enzymes were significantly elevated following CA. Collectively, the results revealed that renal dysfunction following asphyxial CA was strongly associated with the early survival rate and therapeutic hypothermia reduced renal injury via effective antioxidant mechanisms.

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Section: Discussionmentioning

confidence: 85%

Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes

et al. 2021

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“…SOD activity has been shown to decrease in the liver, lung, and ileum in rat models of I/R injury [ 44 - 46 ]. SOD levels decrease after I/R [ 6 ], and the consumption of SODs due to ROS overproduction causes cell damage or death [ 12 ]. We evaluated whether hypothermia treatment was associated with an imbalance antioxidants in the rat liver by examining the expression of antioxidant enzymes (SOD1 and SOD2) by immunohistochemistry and found that immunoreactivities of SOD1 and SOD2 were much higher in the HT/ACA group than in the NT/ACA group.…”

Section: Discussionmentioning

confidence: 99%

“…Consistent with these findings, hypothermic treatment has been reported to offer protection by enhancing the endogenous defense system and increasing the expression of antioxidant enzymes [ 47 ]. In addition, consumption of endogenous antioxidants due to the release of ROS causes apoptotic and necrotic cell death [ 12 ] and hypothermia reduces apoptosis [ 48 ] and necrotic damage at the cellular level by reducing ROS accumulation [ 49 ]. Increased immunoreactivities of liver SOD1 and SOD2 in the HT/ACA group suggest that hypothermia treatment protects against liver inflammation by increasing the expression of antioxidant enzymes that then scavenge oxygen free radicals after I/R.…”

Section: Discussionmentioning

confidence: 99%

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Therapeutic hypothermia reduces inflammation and oxidative stress in the liver after asphyxial cardiac arrest in rats

Park¹,

Ahn²,

Lee

et al. 2020

Acute Crit Care

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Therapeutic hypothermia reduces inflammation and oxidative stress in the liver after asphyxial cardiac arrest in rats Background: Few studies have evaluated the effects of hypothermia on cardiac arrest (CA)induced liver damage. This study aimed to investigate the effects of hypothermic therapy on the liver in a rat model of asphyxial cardiac arrest (ACA). Methods: Rats were subjected to 5-minute ACA followed by return of spontaneous circulation (RoSC). Body temperature was controlled at 33°C± 0.5°C or 37°C ± 0.5°C for 4 hours after RoSC in the hypothermia group and normothermia group, respectively. Liver tissues in each group were collected at 6 hours, 12 hours, 1 day, and 2 days after RoSC. To examine hepatic inflammation, mast cells were stained with toluidine blue. Superoxide anion radical production was evaluated using dihydroethidium fluorescence straining and expression of endogenous antioxidants (superoxide dismutase 1 [SOD1] and SOD2) was examined using immunohistochemistry. Results: There were significantly more mast cells in the livers of the normothermia group with ACA than in the hypothermia group with ACA. Gradual increase in superoxide anion radical production was found with time in the normothermia group with ACA, but production was significantly suppressed in the hypothermia group with ACA relative to the normothermia group with ACA. SOD1 and SOD2 levels were higher in the hypothermia group with ACA than in the normothermia group with ACA. Conclusions: Experimental hypothermic treatment after ACA significantly inhibited inflammation and superoxide anion radical production in the rat liver, indicating that this treatment enhanced or maintained expression of antioxidants. Our findings suggest that hypothermic therapy after CA can reduce mast cell-mediated inflammation through regulation of oxidative stress and the expression of antioxidants in the liver.

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“…Determination of serum oxidative stress index. As previously described (22,23), the content of malondialdehyde (MDA) was detected by thiobarbituric acid colorimetry; the content of GSH and the activity of oxide dismutase (SOD) were determined by chemical methods. The kits for MDA (cat.…”

Section: Methodsmentioning

confidence: 99%

Expression of nuclear factor erythroid‑2‑related factor 2, broad complex‑tramtrack‑bric a brac and Cap‘n’collar homology 1 and γ‑glutamic acid cysteine synthase in peripheral blood of patients with chronic obstructive pulmonary disease and its clinical significance

Sun

Zhu

2021

Exp Ther Med

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The purpose of the present study was to explore the relationship between nuclear factor erythroid 2-related factor 2 (Nrf2)/BTB-CNC allogeneic 1 (Bach1)/γ-glutamic acid cysteine synthase (γ-GCS) and chronic obstructive pulmonary disease (COPD). The expression of Nrf2, Bach1, γ-GCS mRNA and protein in the peripheral blood mononuclear cells (PBMCs) of 80 COPD patients and 40 healthy volunteers were studied. Then, the correlation between Nrf2, Bach1, γ-GCS and lung function, inflammation and oxidative stress indicators was analyzed. Compared with healthy controls, Nrf2, Bach1 mRNA and protein levels were significantly increased in the PBMCs of COPD patients, while γ-GCS mRNA and protein levels were significantly decreased. Nrf2 and Bach1 protein levels in the nucleus were significantly elevated in acute exacerbation COPD patients compared with patients with a stable stage of COPD, while γ-GCS mRNA levels were significantly reduced. In addition, it was found that Nrf2 nuclear protein levels were significantly reduced in COPD patients compared with the control group, while Bach1 nuclear protein levels were significantly increased. Correlation analysis in COPD group demonstrated that γ-GCS mRNA was positively correlated with Nrf2 nuclear protein level, but negatively correlated with Bach1 nuclear protein level. Further analysis demonstrated that γ-GCS mRNA and Nrf2 protein in the nucleus was positively correlated with forced expiratory volume in one second (FEV 1)/forced vital capacity (FVC)% and FEV 1 % predicted, and Bach1 protein in the nucleus was negatively correlated with FEV 1 /FVC% and FEV 1 % predicted. Additionally, the expression levels of Nrf2, Bach1 and γ-GCS were also associated with smoking. The expression of Nrf2, Bach1 and γ-GCS in peripheral blood mononuclear cells of patients with COPD was dysregulated and related to lung function, which provides a new basis for exploring further the pathogenesis of COPD.

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The Hepatoprotective Effect of Aloe Vera on Ischemia-Reperfusion Injury in Rats

Cited by 13 publications

References 27 publications

Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes

Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes

Therapeutic hypothermia reduces inflammation and oxidative stress in the liver after asphyxial cardiac arrest in rats

Expression of nuclear factor erythroid‑2‑related factor 2, broad complex‑tramtrack‑bric a brac and Cap‘n’collar homology 1 and γ‑glutamic acid cysteine synthase in peripheral blood of patients with chronic obstructive pulmonary disease and its clinical significance

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