The effect of activated charcoal dose and benzyl amino purine concentration on the growth of orchid plantlets in murashige and skoog media in vitro

Sipayung, Patricius; Matanari, Jawaller; Lafau, Meryana Br; Sulastri, Yustina Sri; Ginting, Benny; Sihombing, Dewi Restuana; Pandiangan, Maruba; Giawa, Tulusman

doi:10.1088/1755-1315/205/1/012025

Cited by 4 publications

(3 citation statements)

References 2 publications

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“…However, the foundation in providing optimal medium condition is based on nitrogen concentration along with the ammonium/nitrate ratio Koene et al (2019), hence which explains why different mediums need different optimization. Nevertheless, a study by Sipayung et al (2018) stated that activated charcoal does not provide signi icant changes to the growth of plantlets of orchids within two to seven months of culturing in MS media. Other than that, the recommendation of adding charcoal in protocorms development was also supported by the study of Te-Chato et al (2012) whereby 0.2 % of charcoal reduce the browning in the medium for plantlets regeneration.…”

Section: Resultsmentioning

confidence: 99%

Optimization Parameter for Production of Protocorm-Like Body and Growth of Grammatophyllum Speciosum

Rahman

Othman

Amran

et al. 2021

Int. J. Res. Granthaalayah

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Micropropagation of orchids are often through the production of Protocorm Like Bodies (PLBs) and this research conducted a study to discover the optimal condition for the growth of Protocorm Like Bodies (PLBs) and also plantlets regeneration of Grammatophyllum speciosum Several aspects such as different carbon sources (sucrose, maltose) and the presence of charcoal alongside with the variety of concentration of agar were taken into account for the optimization. This study discovered that the 30 g/L of sucrose in 1 mg/L of BAP was superior in producing heavy PLBs. However, the trend differs in the number of plantlets where maltose (5 g/L) is seen to produce a higher number of plantlets compared to sucrose. Charcoal plays an important role in the growth of PLBs where the heaviest PLBs were recorded in the medium supplemented with 0.20 % of charcoal along with 4.5 g of agar. Vice versa relationship was recorded for the number of plantlets where without charcoal in 4.5 g of agar produce the highest number of plantlets.

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Section: Resultsmentioning

confidence: 99%

Optimization Parameter for Production of Protocorm-Like Body and Growth of Grammatophyllum Speciosum

Rahman

Othman

Amran

et al. 2021

Int. J. Res. Granthaalayah

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“…TDZ has obvious inducing effect on cluster buds, compared with 6-BA and KT. Because TDZ belongs to phenylurea cytokinin in structure, it has better induction effect on buds in tissue culture of mature Orchidaceae than that of purine cytokinin (Sipayung et al, 2018). Joshi et al (2008) found that the peanut somatic cell division ability was stronger when treated with high TDZ concentration, and the bud-like protuberance was more obvious, showing radial distribution.…”

Section: Discussionmentioning

confidence: 99%

Appropriate Condition for Cluster Buds Induction of <i>Cremastra appendiculata</i>

Liu¹,

Gao²,

Yang³

et al. 2023

mpb

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To screen for suitable conditions for cluster bud induction of Cremastra appendiculata. The aseptic germination protocorms of C. appendiculata seeds were used as explant materials to explore the effects of different basic media, types of plant growth regulators, anti-browning agents and their concentrations on the induction of cluster buds of C. appendiculata. The results showed that: 1/4MS medium was the suitable basic medium for cluster buds growing; when the concentration of plant growth regulator TDZ was 2.0 mg/L and the concentration of IAA was 0.2 mg/L, the induction rates of cluster bud were higher which are 72.58% and 55.63% respectively; when the anti-browning agent was PVP and its concentration is 2 000 mg/L, the induction rate of cluster buds was higher which is 79.90%. The suitable conditions for C. appendiculata cluster buds induction are: 1/4MS + TDZ 2.0 mg/L+ IAA 0.1 mg/L+ PVP 2 000 mg/L, and the induction rate can reach 81.16%. This provided a basis for establishing a rapid propagation technology system of C. appendiculata and realizing its large-scale artificial cultivation.

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“…En Laelia flava, Miltonia flavescens y Oncidium trulliferum 2 g L -1 de carbón activado en medio MS a la mitad de la concentración de las sales mejoró la calidad de la planta in vitro y favoreció la supervivencia (Moraes et al, 2005). En Cattleya labiata 1.5 g L -1 de carbón activado incrementó el crecimiento de las plántulas in vitro después de ocho meses de cultivo (Sipayung et al, 2018). En Pecteilis radiata la inclusión de carbón activado en el medio de cultivo redujo el oscurecimiento de los protocormos (Kim et al, 2019).…”

Section: Crecimientos De Brotesunclassified

REGENERACIÓN in vitro DE PLANTAS DE Prosthechea vitellina (LINDLEY) W. E. HIGGING POR ORGANOGENESIS DIRECTA

Mora-Cruz

López-Peralta²,

Hernández-Meneses

et al. 2023

RevFitotecMex

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Prosthechea vitellina (Lindley) W. E. Higgins es una orquídea epífita endémica de México con valor ornamental y económico; está sujeta a protección especial por la NOM-059-SEMARNAT-2010 debido al comercio ilegal y la destrucción de su hábitat. El cultivo de tejidos vegetales in vitro es una opción para rescate, conservación y propagación masiva de especies amenazadas. El objetivo del presente estudio fue determinar las condiciones óptimas in vitro para regenerar plantas por organogénesis directa y aclimatarlas. El proceso se inició con plántulas germinadas in vitro, y en las etapas sucesivas se evaluó la concentración de sales del medio MurashigeSkoog (MS, 50 y 100 %), carbón activado, 6-benciladenina (BA, 1.1-2.2 mg L-1), ácido naftalenacético (ANA, 0.19-0.38 mg L-1), ácido indolacético (AIA, 0.18-0.36 mg L-1) y ácido indolbutírico (AIB, 0.5-2.0 mg L-1). En la etapa de aclimatación se evaluaron plantas enraizadas in vitro de 5 y 7 cm en diferentes sustratos. Los experimentos se establecieron en un diseño completamente al azar y los datos obtenidos se sometieron a un ANDEVA. En la etapa de inducción la mayor cantidad de brotes (2.03) se obtuvo con 2.2 mg L-1 de BA y 0.19 mg L-1 de ANA. En la multiplicación se produjeron 2.5 brotes con 1.0 mg L-1 de BA y 0.25 mg L-1 de AIA a las ocho semanas de cultivo. El alargamiento mayor de brotes (2.16 cm) se logró con MS 50 % y 0.5 g L-1 de carbón activado. En el enraizamiento el mayor número de raíces se produjo con 2.0 mg L-1 de AIB, generando 5.27 raíces por planta. En la aclimatación, la supervivencia fue de 100 %, con plantas de 7 cm en corteza de pino. El protocolo desarrollado aporta un método para la propagación de esta orquídea con fines de rescate, conservación, reintroducción y aprovechamiento comercial.

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The effect of activated charcoal dose and benzyl amino purine concentration on the growth of orchid plantlets in murashige and skoog media in vitro

Cited by 4 publications

References 2 publications

Optimization Parameter for Production of Protocorm-Like Body and Growth of Grammatophyllum Speciosum

Optimization Parameter for Production of Protocorm-Like Body and Growth of Grammatophyllum Speciosum

Appropriate Condition for Cluster Buds Induction of <i>Cremastra appendiculata</i>

REGENERACIÓN in vitro DE PLANTAS DE Prosthechea vitellina (LINDLEY) W. E. HIGGING POR ORGANOGENESIS DIRECTA

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