REGENERACIÓN in vitro DE PLANTAS DE Prosthechea vitellina (LINDLEY) W. E. HIGGING POR ORGANOGENESIS DIRECTA

Mora-Cruz, Yazmín; López-Peralta, María Cristina Guadalupe; Hernández-Meneses, Eleodoro; Cruz-Huerta, Nicacio

doi:10.35196/rfm.2023.1.33

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…BA (6-benzylaminopurine) has been the growth regulator par excellence for the optimization of the induction, multiplication, or regeneration stages by direct organogenesis in other species and has recorded more shoots and longer seedlings than MS control. Likewise, depending on the species or variety, and the concentrations or combinations with other regulators, media supplemented with BA are significantly more potent [51][52][53].…”

Section: Experimental Phase 1: In Vitro Multiplicationmentioning

confidence: 99%

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Luz,

Román,

Mar

et al. 2023

Preprint

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the multiplication of seedlings, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL-1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds, shoots, and seedling height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L-1 of BA and 0.1 mg L-1 of TDZ, as well as the MS base culture medium. With 0.1 mg L-1 of BA, 52-mm height, 1.36 shoots, and 9.22 buds were obtained, surpassing the MS control (MS culture medium alone); likewise, twice more roots (80%) and a 14% reduction in the bud structure were packed in 50 repetitions. The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L-1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. The results demonstrated that the induction of callogenesis in S. compositum has a better start from the stem in a medium with 2.0 mg L-1 of 2,4-D, whose value was 4.89 —equivalent to 97.8% callus formation around the explant. The addition of 500 mg L-1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callogenesis of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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Section: Experimental Phase 1: In Vitro Multiplicationmentioning

confidence: 99%

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Luz,

Román,

Mar

et al. 2023

Preprint

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Comparison of in-vitro orchid media solutions for Prosthechea cochleata (L.) W.E. Higgins vars. diandra and triandra

Bartel,

Downing

2024

Plant Cell Tiss Organ Cult

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Prosthechea cochleata or clamshell orchid is recognized as a species of both conservation and commercial importance. It has long been prized by orchid breeders and growers for its unique flower and hardy disposition. The commercial market has failed to meet the demand for this species since it is still targeted for illegal collection in the wild. This study examines the effectiveness of the banana powder on two variants (var. diandra and var. triandra) with disparate home ranges and levels of genetic diversity using two commercially available agar-based media (PhytoTechnology P668 and P748). Undifferentiated protocorms of the two variants were monitored for shoot growth and differentiation across a period of 156 days. The growth media with 3% banana powder supplement (P748) was more effective in initiating shoot formation in both P. cochleata variants compared to control media (P668) (F = 27.15, p < 0.001, df = 81). The diandrous variant grown with banana showed the highest mean shoot count (28.67 shoots/flask) at the end of the monitoring period. Results suggest that banana supplement is an effective source of plant growth regulators and organic nutrients necessary to promote shoot formation and seedling development in this species. This research is important for the conservation of P. cochleata as it identifies an effective and cost-efficient method for in vitro propagation, which can aid in increasing the ex-situ and in situ populations of this threatened species. Additionally, the findings have implications for commercial orchid production, as it can potentially improve the cultivation and yield of this species for economic purposes.

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REGENERACIÓN in vitro DE PLANTAS DE Prosthechea vitellina (LINDLEY) W. E. HIGGING POR ORGANOGENESIS DIRECTA

Cited by 2 publications

References 15 publications

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Comparison of in-vitro orchid media solutions for Prosthechea cochleata (L.) W.E. Higgins vars. diandra and triandra

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