The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep

Hobin, Michael; Dorfman, Katherine; Adel, Mohamed; Rivera-Rodriguez, Emmanuel J.; Kuklin, Elena A.; Ma, Dingbang; Griffith, Leslie C.

doi:10.1016/j.isci.2022.104874

Cited by 2 publications

(1 citation statement)

References 73 publications

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“…Since circadian prothoracicotropic hormone acts as a transcription factor to initiate ecdysteroid signalling, a regulatory circuit of the circadian cycle consisting of the ecdysteroid system and let-7 is established [ 79 ]. It is also worth noting that let-7, miR-375, miR-92a, and bantam are identified as regulating sleep and sleep homeostasis in multiple ways [ 116 , 117 , 118 , 119 , 120 ].…”

Section: Micrornas In Drosophilamentioning

confidence: 99%

Noncoding RNA Regulation of Hormonal and Metabolic Systems in the Fruit Fly Drosophila

Chan

Bendena

et al. 2023

Metabolites

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The importance of RNAs is commonly recognised thanks to protein-coding RNAs, whereas non-coding RNAs (ncRNAs) were conventionally regarded as ‘junk’. In the last decade, ncRNAs’ significance and roles are becoming noticeable in various biological activities, including those in hormonal and metabolic regulation. Among the ncRNAs: microRNA (miRNA) is a small RNA transcript with ~20 nucleotides in length; long non-coding RNA (lncRNA) is an RNA transcript with >200 nucleotides; and circular RNA (circRNA) is derived from back-splicing of pre-mRNA. These ncRNAs can regulate gene expression levels at epigenetic, transcriptional, and post-transcriptional levels through various mechanisms in insects. A better understanding of these crucial regulators is essential to both basic and applied entomology. In this review, we intend to summarise and discuss the current understanding and knowledge of miRNA, lncRNA, and circRNA in the best-studied insect model, the fruit fly Drosophila.

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Section: Micrornas In Drosophilamentioning

confidence: 99%

Noncoding RNA Regulation of Hormonal and Metabolic Systems in the Fruit Fly Drosophila

Chan

Bendena

et al. 2023

Metabolites

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Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner

Kompotis,

Mang,

Hubbard

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression that have been implicated in a plethora of neuronal processes. Nevertheless, their role in regulating brain activity in the context of sleep has so far received little attention. To test their involvement, we deleted mature miRNAs in post-mitotic neurons at two developmental ages, i.e., in early adulthood using conditional Dicer knockout (cKO) mice and in adult mice using an inducible conditional Dicer cKO (icKO) line. In both models, electroencephalographic (EEG) activity was affected and the response to sleep deprivation (SD) altered; while the rapid-eye-movement sleep (REMS) rebound was compromised in both, the increase in EEG delta (1 to 4 Hz) power during non-REMS (NREMS) was smaller in cKO mice and larger in icKO mice compared to controls. We subsequently investigated the effects of SD on the forebrain miRNA transcriptome and found that the expression of 48 miRNAs was affected, and in particular that of the activity-dependent miR-709 . In vivo inhibition of miR-709 in the brain increased EEG power during NREMS in the slow-delta (0.75 to 1.75 Hz) range, particularly after periods of prolonged wakefulness. Transcriptome analysis of primary cortical neurons in vitro revealed that miR-709 regulates genes involved in glutamatergic neurotransmission. A subset of these genes was also affected in the cortices of sleep-deprived, miR-709 -inhibited mice. Our data implicate miRNAs in the regulation of EEG activity and indicate that miR-709 links neuronal activity during wakefulness to brain synchrony during sleep through the regulation of glutamatergic signaling.

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The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep

Cited by 2 publications

References 73 publications

Noncoding RNA Regulation of Hormonal and Metabolic Systems in the Fruit Fly Drosophila

Noncoding RNA Regulation of Hormonal and Metabolic Systems in the Fruit Fly Drosophila

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner

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