Noncoding RNA Regulation of Hormonal and Metabolic Systems in the Fruit Fly Drosophila

Chan, Ki-Kei; Chan, Ting‐Fung; Bendena, William G.; Hui, Jerome H. L.

doi:10.3390/metabo13020152

Cited by 3 publications

(2 citation statements)

References 187 publications

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“…Interestingly, while the shift in the acetylation/methylation ratio may suggest an augmented binding of Pol II, the proportion of the genes exhibiting enhanced Pol II binding was smaller compared to genes with reduced Pol II binding, suggesting additional levels of transcription regulation [33]. The upregulated genes included mir-10, 13 lncRNAs, and the heat-shock protein HSP67Ba, which have reportedly been involved in stress response, possibly activated by the heat shock [34].…”

Section: Discussionmentioning

confidence: 99%

Sedentary Behavior Impacts on the Epigenome and Transcriptome: Lessons from Muscle Inactivation in Drosophila Larvae

Brener,

Lorber,

Reuveny

et al. 2023

Cells

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The biological mechanisms linking sedentary lifestyles and metabolic derangements are incompletely understood. In this study, temporal muscle inactivation in Drosophila larvae carrying a temperature-sensitive mutation in the shibire (shi1) gene was induced to mimic sedentary behavior during early life and study its transcriptional outcome. Our findings indicated a significant change in the epigenetic profile, as well as the genomic profile, of RNA Pol II binding in the inactive muscles relative to control, within a relatively short time period. Whole-genome analysis of RNA-Pol II binding to DNA by muscle-specific targeted DamID (TaDa) protocol revealed that muscle inactivity altered Pol II binding in 121 out of 2010 genes (6%), with a three-fold enrichment of genes coding for lncRNAs. The suppressed protein-coding genes included genes associated with longevity, DNA repair, muscle function, and ubiquitin-dependent proteostasis. Moreover, inducing muscle inactivation exerted a multi-level impact upon chromatin modifications, triggering an altered epigenetic balance of active versus inactive marks. The downregulated genes in the inactive muscles included genes essential for muscle structure and function, carbohydrate metabolism, longevity, and others. Given the multiple analogous genes in Drosophila for many human genes, extrapolating our findings to humans may hold promise for establishing a molecular link between sedentary behavior and metabolic diseases.

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Section: Discussionmentioning

confidence: 99%

Sedentary Behavior Impacts on the Epigenome and Transcriptome: Lessons from Muscle Inactivation in Drosophila Larvae

Brener,

Lorber,

Reuveny

et al. 2023

Cells

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“…In the fruit fly Drosophila melanogaste r, microRNAs have been found to regulate hormonal and metabolic pathways (e.g. Chan et al 2023; Qu et al 2017). Similar to other holometabolous insects, the life cycle of Drosophila exhibits embryo, larva, pupa, and adult stages.…”

Section: Introductionmentioning

confidence: 99%

Insect metamorphosis is regulated differently between sexes by members of a microRNA cluster

Li,

Chan,

Nong

et al. 2024

Preprint

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Insects comprise the majority of all described animal species and dominate the terrestrial habitats. The evolution of insect metamorphosis played a profound role in their successful adaptation and radiation. Insect metamorphosis is dependent on hormones ecdysteroids and sesquiterpenoids such as juvenile hormone. Despite the fact there are genuine differences between sexes during insect metamorphosis which facilitate their successful mating, how such sexual dimorphism in metamorphosis is being controlled is poorly known. We first generated transcriptomic profiles of male and female flies in late larvae and early pupae stages. Using a combination of genome-wide prediction andin vitrodual-luciferase validations, members of a microRNA cluster miR-277/34 were found to potentially regulate the neuropeptide receptor (AstC-R1) that when activated inhibits the sesquiterpenoid pathway and a juvenile hormone-dependent transcription factor (Kr-h1) in flyDrosophila melanogaster.Loss-of-function mutants were created deleting either miR-277 or miR-34, and expression levels of bothAstC-R1andKr-h1as well as ecdysteroid and sesquiterpenoid hormone titres were altered. Further comparison of transcriptomes of the late larvae and early pupae of both sexes revealed differential gene pathways being regulated by members of miR-277/34 between sexes during metamorphosis. This study highlights how members of a microRNA cluster control hormonal and developmental gene pathways in different sexes of insects during metamorphosis.

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The Biological Roles of microRNAs in Drosophila Development

Jang,

Kim,

Shin

et al. 2024

Insects

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Drosophila is a well-established insect model system for studying various physiological phenomena and developmental processes, with a focus on gene regulation. Drosophila development is controlled by programmed regulatory mechanisms specific to individual tissues. When key developmental processes are shared among various insects, the associated regulatory networks are believed to be conserved across insects. Thus, studies of developmental regulation in Drosophila have substantially contributed to our understanding of insect development. Over the past two decades, studies on microRNAs (miRNAs) in Drosophila have revealed their crucial regulatory roles in various developmental processes. This review focuses on the biological roles of miRNAs in specific tissues and processes associated with Drosophila development. Additionally, as a future direction, we discuss sequencing technologies that can analyze the interactions between miRNAs and their target genes, with the aim of enhancing miRNA studies in Drosophila development.

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Noncoding RNA Regulation of Hormonal and Metabolic Systems in the Fruit Fly Drosophila

Cited by 3 publications

References 187 publications

Sedentary Behavior Impacts on the Epigenome and Transcriptome: Lessons from Muscle Inactivation in Drosophila Larvae

Sedentary Behavior Impacts on the Epigenome and Transcriptome: Lessons from Muscle Inactivation in Drosophila Larvae

Insect metamorphosis is regulated differently between sexes by members of a microRNA cluster

The Biological Roles of microRNAs in Drosophila Development

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