The DnaJ OsDjA7/8 is essential for chloroplast development in rice (Oryza sativa)

Zhu, Xiaobo; Liang, Sihui; Yin, Junjie; Yuan, Can; Wang, Jing; Li, Weitao; He, Min; Chen, Weilan; Ma, Bo; Wang, Yuping; Qin, Peng; Li, Shigui; Chen, Xuewei

doi:10.1016/j.gene.2015.07.067

Cited by 32 publications

(17 citation statements)

References 48 publications

(46 reference statements)

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“…In plant, a large number of papers reported that DnaJ proteins regulated growth and development. For Arabidopsis thaliana and rice, DnaJ protein loss led to an obvious late-flowering trait and largely abnormal cellular structures (Shen and Yu, 2011;Zhu et al, 2015). Overexpression of BIL2, one gene encoding the mitochondrial DnaJ protein, promoted elongation of A. thaliana cells by promoting ATP synthesis (Bekhochir et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE

et al. 2020

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Our previous study found that LeDnaJ07 RNAi decreased Lentinula edodes resistance to heat stress and Trichoderma atroviride infection. In this study, the structure and function of the LeDnaJ07 gene was analyzed by gene cloning and overexpression in L. edodes stress-sensitive strain YS55 via the Agrobacterium-mediated transformation method. Transformants were confirmed by qRT-PCR, fluorescence observation and Southern blotting. Overexpression of LeDnaJ07 in YS55 not only enhanced L. edodes mycelial resistance to heat stress but also facilitated mycelial growth. In the presence of heat stress, the intracellular IAA content showed a significant increase in the two LeDnaJ07 overexpression strains but only a slight change in the YS55 wild type strain. Moreover, the interaction between LeDnaJ07 and LetrpE was demonstrated via Y2H and BiFC assays. These results suggested that LeDnaJ07 may be involved in regulating IAA biosynthesis and the resistance of L. edodes to heat stresses via interacting with LetrpE.

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Section: Discussionmentioning

confidence: 99%

Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE

et al. 2020

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“…In addition, DnaJ proteins are essential for normal chloroplast development. Silencing of DnaJ encoded gene OsDjA7/8 in rice resulted in an albino lethal phenotype in the seedling stage due to disordered development of chloroplast [ 101 ]. The detection of HSPs in our transcriptome data has further proven that the expression levels of HSPs are closely related to chloroplast development and Chl biosynthesis in plant species.…”

Section: Discussionmentioning

confidence: 99%

Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling

Shi

et al. 2018

IJMS

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The photosynthetic capacity and efficiency of a crop depends on the biosynthesis of photosynthetic pigments and chloroplast development. However, little is known about the molecular mechanisms of chloroplast development and chlorophyll (Chl) biosynthesis in common wheat because of its huge and complex genome. Ygm, a spontaneous yellow-green leaf color mutant of winter wheat, exhibits reduced Chl contents and abnormal chloroplast development. Thus, we searched for candidate genes associated with this phenotype. Comparative transcriptome profiling was performed using leaves from the yellow leaf color type (Y) and normal green color type (G) of the Ygm mutant progeny. We identified 1227 differentially expressed genes (DEGs) in Y compared with G (i.e., 689 upregulated genes and 538 downregulated genes). Gene ontology and pathway enrichment analyses indicated that the DEGs were involved in Chl biosynthesis (i.e., magnesium chelatase subunit H (CHLH) and protochlorophyllide oxidoreductase (POR) genes), carotenoid biosynthesis (i.e., β-carotene hydroxylase (BCH) genes), photosynthesis, and carbon fixation in photosynthetic organisms. We also identified heat shock protein (HSP) genes (sHSP, HSP70, HSP90, and DnaJ) and heat shock transcription factor genes that might have vital roles in chloroplast development. Quantitative RT-PCR analysis of the relevant DEGs confirmed the RNA-Seq results. Moreover, measurements of seven intermediate products involved in Chl biosynthesis and five carotenoid compounds involved in carotenoid-xanthophyll biosynthesis confirmed that CHLH and BCH are vital enzymes for the unusual leaf color phenotype in Y type. These results provide insights into leaf color variation in wheat at the transcriptional level.

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“…This cucumber albino mutant presented white hypocotyl finally presented lethal performance without developing first true leaf under light condition. In most studies, happening of albino phenotype in plants were caused by absence of chlorophyll and abnormal chloroplast development (27,36,37).…”

Section: Discussionmentioning

confidence: 99%

A novel cucumber albino mutant caused by chloroplast development deficiency

Yan

Liu

Cao

et al. 2020

Preprint

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Background Photosynthesis is a fundamental process for plant growth and development dependent on a precise network, including formation of chloroplast and chlorophyll synthesis. Chloroplast development deficiency could lead to albinism in higher plant. Results Here, we report a cucumber albino recessive mutant that processed white cotyledons under light condition and is unable to produce first true leaf. Meanwhile, albino mutant could grow out creamy green cotyledons under dark condition but died after exposing to light. Using fluorescence microscopy and transmission electron microscope (TEM), impaired chloroplasts were observed. We identified 7 and 3 differentially expressed genes (DEG) involved in Chlorophyll metabolism and Methylerythritol 4-phosphate (MEP) pathway through transcriptome analysis, respectively. We also examined the reported homologous genes for albino mutants from other plants. Two of 12 genes, TOC159 and DXS1, were up-regulated in cucumber albino mutants as well. The reliability of RNA sequencing results were further confirmed by real-time quantitative PCR (qPCR). Conclusions Taken together, we elaborate the differences between albino mutant and normal seedlings from a single cucumber progeny. This mutant is a new material to study protoplast development.

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The DnaJ OsDjA7/8 is essential for chloroplast development in rice (Oryza sativa)

Cited by 32 publications

References 48 publications

Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE

Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE

Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling

A novel cucumber albino mutant caused by chloroplast development deficiency

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