Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE

Wang, Jinjie; Luo, Yi; Wang, Chen; Zhou, Yan; Mou, Chunye; Kang, Heng; Xiao, Yang; Gong, Yu

doi:10.3389/fmicb.2020.00707

Cited by 14 publications

(11 citation statements)

References 34 publications

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“…The pre-treatment of pea plants with auxins TA-12 and TA-14 could mitigate the oxidative stress provoked by high-temperature treatment ( Sergiev et al, 2018 ). This is consistent with our previous results: (1) exogenous addition of IAA could significantly increase the thermotolerance of heat-sensitive strain YS3357, with a significant increase in the IAA content of mycelium under heat stress, and higher endogenous IAA content in the heat-tolerant strain than the heat-sensitive strain ( Wang et al, 2018b , 2020 ); (2) exogenous 2,4-D could improve the thermotolerance of L. edodes strain YS3357 by increasing mycelial antioxidant enzyme activity and reducing cellular lipid peroxidation degree ( Zhou et al, 2018b ).…”

Section: Discussionsupporting

confidence: 93%

“…Previous studies have shown that Para-aminobenzoic acid (PABA) synthase, nitric oxide, and trehalose can reduce the accumulation of reactive oxygen species (ROS) to alleviate oxidative damage induced by heat stress, thereby improving the thermotolerance of a variety of macrofungi ( Kong et al, 2012 ; Yin et al, 2015 ; Luo et al, 2021 ). In our previous studies, LetrpE , LeYUCCA8 , LeDnaJ07 , auxin, and 2,4-Dichlorophenoxyacetic acid (2,4-D) were found to participate in the response of L. edodes to heat stress ( Zhou et al, 2018a ; Wang et al, 2018b , 2020 ; Zhong et al, 2021 ). 2,4-D, a functional analogue of the auxin indole-3-acetic acid (IAA) is widely used as an IAA substitute because it can induce IAA like activities ( Maher and Martindale, 1980 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis Provides Insights Into the Mechanism by Which 2,4-Dichlorophenoxyacetic Acid Improves Thermotolerance in Lentinula edodes

Zhou

Song

et al. 2022

Front. Microbiol.

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As the widest cultivated edible mushroom worldwide, Lentinula edodes suffers serious yield and quality losses from heat stress during growth and development, and in our previous study, exogenous 2,4-Dichlorophenoxyacetic acid (2,4-D) was found to improve the thermotolerance of L. edodes strain YS3357, but the molecular mechanism remains unclear. Here, we explored the potential protective mechanism of exogenous 2,4-D against heat stress by transcriptome analysis. 2,4-D possible improve the thermotolerance of L. edodes through regulating antioxidant genes, transcription factors, energy-provision system, membrane fluidity, and cell wall remodeling. Furthermore, 2,4-D was also found to regulate the saturation levels of fatty acids and ATP content in L. edodes mycelium under heat stress. This study proposed a regulatory network of 2,4-D in regulating L. edodes response to heat stress, providing a theoretical basis for improving L. edodes thermotolerance, and facilitating the understanding of the molecular mechanism of exogenous hormones in alleviating abiotic stress damage to macrofungi.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Provides Insights Into the Mechanism by Which 2,4-Dichlorophenoxyacetic Acid Improves Thermotolerance in Lentinula edodes

Zhou

Song

et al. 2022

Front. Microbiol.

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“…Heat stress is a major abiotic constraint in mycelial growth and fruiting body productivity for macrofungi [ 15 , 16 ]. Direct injuries that result from high temperatures include cell wall damage [ 17 ], increased membrane fluidity and permeability [ 18 , 19 ], nuclear condensation, mitochondrial dysfunction, and DNA fragmentation [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Upon exposure to heat, heat response factors (HSFs) can boost the transcription and accumulation of heat-stimulated gene products by interacting with heat stress-related genes [ 28 ]. In L. edodes , it was reported that HSPs could enhance thermotolerance by regulating IAA biosynthesis [ 15 , 25 , 29 , 30 , 31 ]. To date, however, the differences in gene expression and metabolic adaptation for different temperature types of L. edodes in response to high temperature stress have not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Two Strains of Lentinula edodes Differ in Their Transcriptional and Metabolic Patterns and Respond Differently to Thermostress

Guo

Gao

Fan

et al. 2023

JoF

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Temperature type is one of the key traits determining the cultivation regime of Lentinula edodes. However, the molecular and metabolic basis underling temperature type remain unclear. Here, we investigated the phenotypic, transcriptomic, and metabolic features of L. edodes with different temperature types under both control (25 °C) and high (37 °C) temperature conditions. We found that under the control condition, the high- and low-temperature types of L. edodes harbored distinct transcriptional and metabolic profiles. The high-temperature (H-)-type strain had a higher expression level of genes involved in the toxin processes and carbohydrate binding, while the low-temperature (L-)-type strain had a high expression level of oxidoreductase activity. Heat stress significantly inhibited the growth of both H- and L-type strains, while the latter had a higher growth inhibition rate. Upon exposure to heat, the H-type strain significantly up-regulated genes associated with the components of the cellular membrane, whereas the L-type strain markedly up-regulated genes involved in the extracellular region and carbohydrate binding. Metabolome data showed that thermostress altered purine and pyrimidine metabolism in the H-type strain, whereas it altered cysteine, methionine, and glycerophospholipid metabolism in the L-type strain. Transcriptome and metabolome integrative analysis was able to identify three independent thermotolerance-related gene–metabolite regulatory networks. Our results deepen the current understanding of the molecular and metabolic basis underlying temperature type and suggest, for the first time, that thermotolerance mechanisms can be temperature-type-dependent for L. edodes.

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“…RT-PCR analysis after high-temperature stress showed that the expression of Hsp40 , Hsp60 , Hsp70 , Hsp90 and Hspl00 was higher in heat-tolerant strains than in heat-sensitive strains of L. edodes ( Xin et al, 2016 ; Wang et al, 2018a ). Some domestic studies have shown that the addition of exogenous auxins can improve L. edodes mycelial thermotolerance ( Zhou et al, 2018 ); and have studied knockdown and overexpression of the anthranilate-synthase gene (TrpE) in L. edodes to investigated its role during heat stress ( Ma et al, 2018 ; Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Construction of a heat-resistant strain of Lentinus edodes by fungal Hsp20 protein overexpression and genetic transformation

Ling

Zhao

2022

Front. Microbiol.

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The shiitake mushroom (Lentinus edodes) is the second most popular edible mushroom globally due to its rich nutritional value and health benefits associated with consumption. However, the characteristics of growing at low temperatures limit the area and time of its cultivating. We selected a low-temperature cultivar as the original strain. We proposed to construct a heat-shock protein expression vector to achieve genetic transformation in this low-temperature strain to improve the survivability of the strain against the heat-shock response. In this study, an overexpression vector pEHg-gdp-hsp20 for the heat shock protein 20 gene of A. bisporus was constructed using a homologous recombination method. This vector was transferred into dikaryotic and monokaryotic mycelia by the Agrobacterium tumefaciens-method. The integration of hygb and hsp20 into the genome of L. edodes mycelia was verified by growth experiments on resistant plates and PCR analysis. The expression of the reporter gene mgfp5 was verified by fluorescence microscopy analysis and statistically resulted in 18.52 and 26.39% positivity for dikaryon, and monokaryon, respectively. Real-time PCR analysis showed that the expression of the hsp20 gene was more than 10-fold up-regulated in the three transformants; the mycelia of the three overexpression transformants could resume growth after 24 h heat treatment at 40°C, but the mycelia of the starting strain L087 could not recover growth at 25°C indicating that strains that successfully expressed hsp20 had greater overall recovery after heat shock. According to the study, A. bisporus hsp20 gene overexpression effectively improves the defensive capability of low-temperature mushroom strains against heat shock, laying the foundation for breeding heat-resistant high-quality transgenic shiitake mushrooms.

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Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE

Cited by 14 publications

References 34 publications

Comparative Transcriptome Analysis Provides Insights Into the Mechanism by Which 2,4-Dichlorophenoxyacetic Acid Improves Thermotolerance in Lentinula edodes

Comparative Transcriptome Analysis Provides Insights Into the Mechanism by Which 2,4-Dichlorophenoxyacetic Acid Improves Thermotolerance in Lentinula edodes

Two Strains of Lentinula edodes Differ in Their Transcriptional and Metabolic Patterns and Respond Differently to Thermostress

Construction of a heat-resistant strain of Lentinus edodes by fungal Hsp20 protein overexpression and genetic transformation

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