The distribution of xylose in the cells of ballistosporous yeasts - Application of high performance liquid chromatography without derivatization to the analysis of xylose in whole cell hydrolysates.

Abstract

The presence or absence of xylose in the whole cell hydrolysates from all type strains of 41 known species of the genera Bu//era, Sporidiobo/us, and Sporobolomyces, and three strains of Itersonilia perplexans was examined using high performance liquid chromatography (HPLC) which makes it possible to analyze the carbohydrates without derivatization. Of 16 species of the genus Bu//era, 14 had xylose in the cells. The other two species, Bu//era salicina and Bu//era tsugae, had no xylose in the cells. None of the 25 species of the genera Sporidiobolus and Sporobolomyces had xylose in the cells. The lack of xylose in the cells of seven Sporobolomyces species with Q-9 as the major ubiquinone proves that these species are not yeast phases of certain species of the genus Itersonilia with Q-9, since both the yeast and filamentous phases of Itersonilia perplexans had xylose in the cells. These results show that the presence or absence of xylose in the cells is very useful as one criterion for the generic differentiation of Bu//era and Sporobolomyces. The lack of xylose in the cells of B. salicina and B. tsugae indicates that these two species may be closely related to the genus Sporobolomyces. Our HPLC method, by which xylose in the whole cell hydrolysates can be analyzed without derivatization, is a useful way to differentiate taxa by this criterion.Cellular carbohydrate composition provides useful taxonomic information at the level of genus or higher taxa of yeasts and yeast-like fungi (1-7). WEIJMAN and RODRIGUES DE MIRANDA (5) pointed out the significance of the presence or absence

Candida stellimalicola, a new species of anamorphic yeast isolated from star apple in Thailand.

“…Cell wall polysaccharide was extracted and purified as a copper complex according to the method of Gorin and Spencer (1). Suger composition of purified polysaccharide was analyzed by HPLC method described by Suzuki and Nakase (8). Proton magnetic resonance (PMR) spectra of cell wall polysaccharides were determined with 20o solution in deuterium oxide at 70°C with a JNM-GX 500 MHz nuclear magnetic resonance spectrometer (JEOL Ltd., Tokyo, Japan) in which 3-(trimethylsilyl) propionic acid-d4 sodium salt was employed as the internal standard.…”

Bensingtonia ingoldii sp. nov., a ballistospore-forming yeast isolated from Knightia excelsa collected in New Zealand.

“…Xylose in the cells was analyzed by HPLC as previously described using whole cell hydrolyzate (11). RESULTS …”

Molecular phylogeny of the ballistoconidium-forming anamorphic yeast genus bullera and related taxa based on small subunit ribosomal DNA sequences.

“…However, their data were not clear-cut and some discrepancies were found at that time. Later, in 1988, Suzuki and Nakase (23) analyzed monosaccharide composition of whole-cell hydrolysates of species of Bullera and Sporobolomyces, including species of Sporidiobolus, which is considered to be a teleomorph of Sporobolomyces. They found that these genera were clearly separated according to the presence or absence of cellular xylose.…”