Molecular phylogeny of the ballistoconidium-forming anamorphic yeast genus bullera and related taxa based on small subunit ribosomal DNA sequences.

1998

Mycoscience

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Small subunit ribosomal DNA sequences of all species of the basidiomycetous anamorphic yeast genus Trichosporon were determined, and phylogenetic trees were constructed by the neighbor-joining and maximum likelihood methods.The sequence data showed that, with the exception of T. pullulans, the genus is monophyletic, although its members have two different major ubiquinones, Q9 and QIO. The genus can be divided phylogenetically into three major clusters. Species with QIO as the major ubiquinone constitute a single cluster, while those with Q9 form two clusters. Trichosporon pullulans was phylogenetically distinct from other taxa of the genus. It is located in a cluster containingCystofilobasidium capitatum, Mrakia frigida, Xanthophy#omyces dendrorhous and three species of Udeniomyces. This result suggests that T. pullulans does not belong to the genus Trichosporon.

Section: Resultsmentioning

confidence: 99%

Section: Strains Usedmentioning

confidence: 99%

Molecular phylogenetic study of the basidiomycetous anamorphic yeast genus Trichosporon and related taxa based on small subunit ribosomal DNA sequences

Sugita

1998

Mycoscience

Self Cite

“…The SSU rRNA coding region was amplified by the PCR as described in Suh & Nakase (1995). The PCR product was sequenced by using the Sequi Therm Long-Read Cycle Sequencing Kit (Epicentre Technologies) with the primers described in Suh et al (1996). Analyses of DNA sequence reactions were performed on an ALFexprc.ss DNA sequencer (Pharmacia Bi o t ec h) .…”

Section: Methodsmentioning

confidence: 99%

Trichosporon japonicum sp. nov. isolated from the air

Sugita

International Journal of Systematic Bacteriology

1998

A yeast strain isolated from the air in Japan was found to represent a new species, and was named Trichosporon japonicum. This species produced arthroconidia and appressoria. T. japonicum formed a cluster with the appressorium-forming species Trichosporon inkin and Trichosporon ovoides in a phylogenetic tree constructed using small-subunit rDNA sequences. However, they had low relatedness to each other in DNA-DNA hybridization experiments. T. japonicum is distinguished from both T. inkin and T. ovoides by its ability to assimilate inulin, and its inability to assimilate L-rhamnose. JCM 8357T is the type strain of the species.

“…The PCR products were cloned directly in the linearized pDIRECT vector (Clontech) and the double-stranded DNA templates for sequencing were prepared according to the directions of the manufacturer. Sequencing was performed by using the Pharmacia Autocycle Sequencing kit (Taq DNA polymerase) as described by Suh et al (10,11).…”

Section: Methodsmentioning

confidence: 99%

Fellomyces ogasawarensis sp. nov. and Fellomyces distylii sp. nov., yeasts isolated from a plant in Japan

Hamamoto

Kuroyanagi

International Journal of Systematic Bacteriology

1998

Fellomyces ogasawarensis and Fellomyces distylii, new yeast species isolated from a dead leaf of a plant (Distylium lepidotum Nakai, family Hamamelidaceae) collected in the Ogasawara Islands, isolated islands in the Pacific Ocean about 1000 km south of Japan, are described. The phylogenetic relationship of F. ogasawarensis and F. distylii with other members of the genus Fellomyces was estimated from 185 rRNA gene sequence analysis. The type strain of F. ogasawarensis is strain OK-81 (= JCM 9861) and that of F. distylii is strain OK-83 (= JCM 9862).