Molecular phylogenetic study of the basidiomycetous anamorphic yeast genus Trichosporon and related taxa based on small subunit ribosomal DNA sequences

Abstract

Small subunit ribosomal DNA sequences of all species of the basidiomycetous anamorphic yeast genus Trichosporon were determined, and phylogenetic trees were constructed by the neighbor-joining and maximum likelihood methods.The sequence data showed that, with the exception of T. pullulans, the genus is monophyletic, although its members have two different major ubiquinones, Q9 and QIO. The genus can be divided phylogenetically into three major clusters. Species with QIO as the major ubiquinone constitute a single cluster, while those with Q9 form two clusters. Trichosporon pullulans was phylogenetically distinct from other taxa of the genus. It is located in a cluster containingCystofilobasidium capitatum, Mrakia frigida, Xanthophy#omyces dendrorhous and three species of Udeniomyces. This result suggests that T. pullulans does not belong to the genus Trichosporon.

Phylogenetic and Taxonomic Heterogeneity of Cryptococcus humicolus by Analysis of the Sequences of the Internal Transcribed Spacer Regions and 18S rDNA, and the Phylogenetic Relationships of C. humicolus, C. curvatus, and the Genus Trichosporon

“…Ikeda et al (6) and Nishiura et al (13) found four different serotypes (I, II, III and I-III) of Trichosporon species in a serological analysis using specific antisera. These four serotypes also corresponded well to the phylogenetic groups (16,17). High bootstrap values supported the C. humicolus cluster with C. curvatus and the genus Trichosporon in both the Neighbor-Joining and Maximum Parsimony trees.…”

A Nested PCR Assay to Detect DNA in Sera for the Diagnosis of Deep‐Seated Trichosporonosis

“…The primer sequences were designed by analyzing the sequences 529 bp of the 18S and 5.8S rONA and internal transcribed spacer (ITS) regions I and 2, previously determined by Sugita et al (15) and Sugita and Nakase (13). Nested PCR was conducted with two sets of primers, as shown in Table 4.…”

Two new yeasts, Trichosporon debeurmannianum sp. nov. and Trichosporon dermatis sp. nov., transferred from the Cryptococcus humicola complex.

“…ho et al, 1992 ;Sugita et al, 1995a ;Sugita & Nakase, 1998a ;Middelhoven et al, 1999Middelhoven et al, , 2000. Sugita & Nakase (1998b) and Fell et al (2000) reported that the genus Trichosporon was monophyletic on the basis of 18S and 28S (D1\D2 regions) rDNA sequences, and each cluster correlated well with the molecular type of ubiquinone. Specifically, of three main clusters, one consists of the species possessing Q10 and the remaining two possess Q9.…”