The distribution of tobacco HRS60 DNA repeated sequences in species of the genus Nicotiana

Telomerase-Dependent 3′ G-Strand Overhang Maintenance Facilitates GTBP1-Mediated Telomere Protection from Misplaced Homologous Recombination

“…Antibody-bound nucleoproteins were collected using protein G agarose beads (Santa Cruz Biotechnology). Eluted DNA was dot blotted onto Hybond-N nylon membranes (Amersham) and hybridized with 32 P-labeled (TTTAGGG) 70 or HRS 60 (Koukalova et al, 1993) probes.…”

The Horizontal Transfer of Agrobacterium rhizogenes Genes and the Evolution of the Genus Nicotiana

“…1). More recently a further characterization of the genus has been carried out with physiological and molecular tools like the analysis of the morphophysiological in vitro behavior of explants from several species (Bogani et al, 1985(Bogani et al, , 1997 and studies of DNA multiplicity changes (Narayan, 1987) and of interspecific sequence variation through the use of a series of molecular markers like chloroplast RFLP, repeated sequences, and RAPD polymorphisms (Kung et al, 1982;Tassopulu and Kung, 1984;Koukalova et al, 1993;Komarinski et al, 1995;Hua et al, 1993;Borisjuk et al, 1994;Bogani et al, 1997;Yu and Lin, 1997).…”

A Physiological and Molecular Analysis of the GenusNicotiana

“…Therefore a vast amount of data is available on the morphology and cytogenetics of the genus (Goodspeed, 1954) along with evidence on the role of DNA multiplicity changes during evolution (Narajan, 1987), on the distribution of repeated sequences (Koukalovà et al, 1993), and on chloroplast DNA modifications (Kung et al, 1982;Rhodes et al, 1981). Moreover at the physiological level, a vast amount of studies have been carried out on the relevance of auxin/cytokinin equilibria for tumor formation in hybrids between species known to have different phylogenetic histories (Kostoff, 1930;Ahuja, 1968;Smith, 1968;Nä f, 1958).…”