The Horizontal Transfer of Agrobacterium rhizogenes Genes and the Evolution of the Genus Nicotiana

Intrieri, Maria; Buiatti, M.

doi:10.1006/mpev.2001.0927

Cited by 96 publications

(92 citation statements)

References 21 publications

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“…These T-DNA sequences, originating from different A. rhizogenes strains, are present in Nicotiana spp., are transmitted to the progeny, and are expressed at low levels but are not associated with the hairy root or tumor-like symptoms that are usually induced by Agrobacterium infections (23)(24)(25). The Rol genes in Nicotiana glauca appear to play a role in various developmental processes and in the expression of morphologic characteristics that have contributed to the evolution of the genus (23,26). A PCR and hybridization-based search for naturally transgenic species in the family Solanaceae was conducted without success (23).…”

Section: Significancementioning

confidence: 99%

“…The Rol genes in Nicotiana glauca appear to play a role in various developmental processes and in the expression of morphologic characteristics that have contributed to the evolution of the genus (23,26). A PCR and hybridization-based search for naturally transgenic species in the family Solanaceae was conducted without success (23). However, a subsequent, more extensive search resulted in the identification of A. rhizogeneshomologous T-DNA sequences in Linaria vulgaris (27,28).…”

Section: Significancementioning

confidence: 99%

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The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop

Kyndt

Quispe

Zhai

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

241

161

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Agrobacterium rhizogenes and Agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring DNA fragments [transfer DNA (T-DNA)] bearing functional genes into the host plant genome. This naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their use can result in considerable controversy. While assembling small interfering RNAs, or siRNAs, of sweet potato plants for metagenomic analysis, sequences homologous to T-DNA sequences from Agrobacterium spp. were discovered. Simple and quantitative PCR, Southern blotting, genome walking, and bacterial artificial chromosome library screening and sequencing unambiguously demonstrated that two different T-DNA regions (IbT-DNA1 and IbT-DNA2) are present in the cultivated sweet potato (Ipomoea batatas [L.] Lam.) genome and that these foreign genes are expressed at detectable levels in different tissues of the sweet potato plant. IbT-DNA1 was found to contain four open reading frames (ORFs) homologous to the tryptophan-2-monooxygenase (iaaM), indole-3-acetamide hydrolase (iaaH), C-protein (C-prot), and agrocinopine synthase (Acs) genes of Agrobacterium spp. IbT-DNA1 was detected in all 291 cultigens examined, but not in close wild relatives. IbT-DNA2 contained at least five ORFs with significant homology to the ORF14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ ORF17n genes of A. rhizogenes. IbT-DNA2 was detected in 45 of 217 genotypes that included both cultivated and wild species. Our finding, that sweet potato is naturally transgenic while being a widely and traditionally consumed food crop, could affect the current consumer distrust of the safety of transgenic food crops.horizontal gene transfer | Agrobacterium spp. | food safety | sweet potato | transgenic crops

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Section: Significancementioning

confidence: 99%

Section: Significancementioning

confidence: 99%

The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop

Kyndt

Quispe

Zhai

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

241

161

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“…При характеристике морфогенетических процес-сов в культуре in vitro было показано, что большинство видов из группы «+» склонно к побегообразованию, в то время как для представителей группы «-» характерна склонность к образованию корней [3]. У предста-вителей группы «-» обнаружены последовательности, подобные Т-ДНК из Agrobacterium rhizogenes (гены, гомологичные rolB, rolC, ORF13, ORF14), как результат горизонтального переноса генов в процессе эво-люции [13,7]. В пределах группы «+» таковых не обнаружено.…”

Section: Introductionunclassified

Characteristics of morphogenetic responses of Nicotiana langsdorffii on transformation with wild type strains of Agrobacterium in vitro

Матвеева

Pigichka

Лутова

2007

Ecological genetics

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Ability to transformation by wt strains of Agrobacterium tumefaciens (T37, C58, A6) and A. rhizogenes (15834, 8196, A4) was characterized for Nicotiana langsdorffii. It was shown that effectivity of transformation of this species by strains T37, A6,15834, 8196, A4 was lower comparing to the model object N. tabacum. Tumors induced by A. tumefaciens on leaf disks of N. langsdorffii, tend to shoot formation.

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“…Sola naceae был проведен только у представителей рода Nicotiana и нескольких видов дру-гих родов (Intrieri and Buiatti, 2001). Методом блот-гибридизации геномной ДНК по Саузерну и ПЦР онкогеноподобные последовательности были вы-явлены у N. glauca, N. cordifolia, N. debneyi.…”

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“…Интересно отметить, что дан-ные последовательности обнаружены у всех исследованных представителей указанных видов, вне зависимости от экологических и географических условий их произрастания. То есть наличие Т-ДНК в геноме является характеристикой вида (White et al, 1983;Furner et al, 1986;Meyer et al, 1995;Frundt et al, 1998;Intrieri, Buiatti, 2001).…”

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Investigations of possible horizontal gene transfer from Agrobacterium to some representatives of the family Solanaceae

Kulayeva

Алексеевна²,

Matveeva

et al. 2013

Ecological genetics

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The development of methods of genetic engineering of plants poses in modern society the question of the safety of transgenic plants. Transformation of plants has been criticized as artificial process performed only in the laboratory. At the same time there is evidence that some members of the genus Nicotiana and Linaria contain sequences homologous to the T-DNA of Agrobacterium rhizogenes in their genomes. The question: whether there are other examples of horizontal gene transfer from Agrobacterium to the plant among the representatives of the family. Solanaceae, is still actual. We performed screening of representatives of four genera of the family Solanaceae for the presence of sequences homologous to oncogeniclike genes rolB, rolC, ORF13, ORF14. New examples of horizontal gene transfer were not detected. Apparently, the presence of sequences homologous to oncogenes of A. rhizogenes in the genome of nontransformed plants is not common to the whole family Solanaceae and is characteristic only of the some species of the genus Nicotiana.

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The Horizontal Transfer of Agrobacterium rhizogenes Genes and the Evolution of the Genus Nicotiana

Cited by 96 publications

References 21 publications

The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop

The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop

Characteristics of morphogenetic responses of Nicotiana langsdorffii on transformation with wild type strains of Agrobacterium in vitro

Investigations of possible horizontal gene transfer from Agrobacterium to some representatives of the family Solanaceae

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