The diffusion limited oxidase-based glucose enzyme electrode: relation between covering membrane permeability and substrate response

Koochaki, Zahra; Higson, Séamus P.J.; Mutlu, Mehmet; Vadgam, Pankaj M.

doi:10.1016/0376-7388(93)85223-j

Cited by 19 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown that thin-polymer-®lm-composite membranes exhibiting lower glucoseaoxygen P ratios are associated with extended linearity ranges when used as the upper membrane in glucose oxidase enzyme electrodes [4,6,7].…”

Section: Resultsmentioning

confidence: 99%

“…Conventional diffusion chamber apparatus was used (as previously described [7,8]) to follow the mass transport of glucose and oxygen across a range of thin-polymer-®lm composite membrane structures to determine the permeability coef®cients of both glucose and oxygen. A glucose enzyme electrode was fabricated in order to monitor glucose concentrations of aliquots periodically extracted from one side of the diffusion chamber, again as previously described [7].…”

Section: Determination Of Solute Permeability Coef®cientsmentioning

confidence: 99%

“…A glucose enzyme electrode was fabricated in order to monitor glucose concentrations of aliquots periodically extracted from one side of the diffusion chamber, again as previously described [7]. Oxygen levels were monitored by ®rst removing aliquots of buffer from one side of the diffusion chamber and then determining O 2 concentrations via the use of an O 2 electrode, as previously described [7].…”

Section: Determination Of Solute Permeability Coef®cientsmentioning

confidence: 99%

See 2 more Smart Citations

Polyacrylonitrile Thin-Film Composite Membranes for the Optimization of a Whole Blood Glucose Sensor

Bridge

Higson

2001

Electroanalysis

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Section: Determination Of Solute Permeability Coef®cientsmentioning

confidence: 99%

Section: Determination Of Solute Permeability Coef®cientsmentioning

confidence: 99%

See 1 more Smart Citation

Polyacrylonitrile Thin-Film Composite Membranes for the Optimization of a Whole Blood Glucose Sensor

Bridge

Higson

2001

Electroanalysis

View full text Add to dashboard Cite

“…For example, the reaction of glucose with GOD in various conditions was intensively examined in our biosensor studies. In a preliminary analysis, the diffusion of GOD in a biological matrix, porcine mucus, was examined [29]. In another study, we reported the diffusion-limited reaction kinetics of GOD and glucose for sandwich-type enzyme electrode applications [30].…”

Section: Effect Of Enzyme Concentrationmentioning

confidence: 99%

Biocatalytic protein membranes fabricated by electrospinning

Kabay

Kaleli

Sultanova

et al. 2016

Reactive and Functional Polymers

Self Cite

View full text Add to dashboard Cite

In this study, a protein-based catalytic membrane was produced by electrospinning. Membrane activity was characterised in terms of response current for various glucose concentrations. We focused on the preparation of a scaffold by converting a globular protein to other structural forms using catastrophic solvents. A scaffolding protein, bovine serum albumin, and an enzyme, glucose oxidase (GOD), were selected as a model natural carrier matrix and a biologically active agent, respectively. Beta-mercaptoethanol (β-ME) was used to convert the globular protein to an amyloid-like form. A structural stabilising agent, 2,2,2-triflouroethanol (TFE), was used to maintain the final α-helical structure of the amyloid-like protein. The TFE:PBS (phosphate-buffered saline) ratio and various electrospinning parameters were analysed to minimise activity loss. Using this approach, we applied electrospinning to an active enzyme to obtain biocatalytic nanofibrous membranes. After optimising the protein electrospinning process, the activities of the protein nanofibrous membranes were monitored. GOD remained active in the new membrane structure. The highest enzyme activity was observed for the membranes prepared with a 1.5:1 (v:v) TFE:PBS solvent ratio. In that particular case, the immobilized enzyme created a current of 0.7 μA and the apparent activity was 2547 ± 132 U/m 2 .

show abstract

“…Polycarbonate track-etched membrane (PTEM) was a membrane with high strength, low adsorption, and high filtration performance, which could be used to replace the multicapillary glass plate . PTEMs were formed by preirradiation of polymer films with charged ions, and were extensively used for ultrafiltration and purification, , while in the field of diagnosis, their application was explored for sensors . In addition, to avoid relying on expensive instruments as used in ELISA, studies on reading of results were attempted. , The transmitted light from a microwell plate was imaged using the camera of a smartphone and then the image was analyzed.…”

mentioning

confidence: 99%

Smartphone-Based High-Throughput Fiber-Integrated Immunosensing System for Point-of-Care Testing of the SARS-CoV-2 Nucleocapsid Protein

Liu

Liang

et al. 2022

ACS Sens.

View full text Add to dashboard Cite

To control the coronavirus disease 2019 (COVID-19) pandemic, there is an urgent need for simple, rapid, and reliable detection methods to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, especially in community hospitals or clinical centers. The SARS-CoV-2 nucleocapsid protein (NP) is an important index for diagnosis of COVID-19. Here, we proposed a smartphone-based high-throughput fiber-integrated immunosensing system (HFIS) for detecting the SARS-CoV-2 NP in serum samples within 45 min. For the testing of NP standards, the linear detection range was 7.8−1000 pg/mL, the limit of detection was 7.5 pg/mL, and the cut-off value was 8.923 pg/mL. Twenty-five serum samples from clinically diagnosed COVID-19 patients and 100 negative control samples from healthy blood donors were tested for SARS-CoV-2 NP by HFIS, and the obtained results were compared with those of ELISA and Simple Western analysis. The results showed that the HFIS sensitivity and specificity were 72% [95% confidence interval (CI): 52.42−85.72%] and 100% (95% CI: 96.11−100%), respectively, which significantly correlated with those from the commercial ELISA kit and Simple Western analysis. This portable high-throughput HFIS assay could be an alternative test for detecting SARS-CoV-2 NP in blood samples on site.

show abstract

The diffusion limited oxidase-based glucose enzyme electrode: relation between covering membrane permeability and substrate response

Cited by 19 publications

References 9 publications

Polyacrylonitrile Thin-Film Composite Membranes for the Optimization of a Whole Blood Glucose Sensor

Polyacrylonitrile Thin-Film Composite Membranes for the Optimization of a Whole Blood Glucose Sensor

Biocatalytic protein membranes fabricated by electrospinning

Smartphone-Based High-Throughput Fiber-Integrated Immunosensing System for Point-of-Care Testing of the SARS-CoV-2 Nucleocapsid Protein

Contact Info

Product

Resources

About