The dietary valine requirement for rainbow trout,Oncorhynchus mykiss, can be estimated by plasma free valine and ammonia concentrations after dorsal aorta cannulation

Abstract

This study was carried out to evaluate the dietary valine requirement by measuring means of the plasma free valine and ammonia concentrations in rainbow trout, Oncorhynchus mykiss, after dorsal aorta cannulation. A total of 35 fish averaging 49897.2 g (initial body weight, mean9SD) were randomly distributed into seven groups with five fish in each group. After 48 h of feed deprivation, each group was fed one of seven L-amino acid based diets containing graded levels of valine (0.45, 0.95, 1.20, 1.45, 1.70, 1.95 or 2.45% of diet, dry matter basis) by intubation at 1% body weight. Blood samples were taken at 0, 5 and 24 h after intubation. Post-prandial plasma free valine concentrations (PPval, 5 h after intubation) and post-absorptive free valine concentrations (PAval, 24 h after intubation) of fish fed diets containing 1.45% or more valine were significantly higher than those of fish fed diets containing 1.20% or less valine (PB0.05). Post-prandial plasma ammonia concentrations (PPA, 5 h after intubation) remained low or constant from fish fed diets containing 0.45Á1.45% valine, but increased linearly from fish fed diets containing 1.45Á2.45% valine. Post-absorptive plasma ammonia concentrations in the present study were not significantly different among the groups. Broken-line model analyses on PPval, PAval and PPA indicated that the dietary valine requirements of rainbow trout was between 1.41 (3.85) and 1.50% (4.10) of diet (% of dietary protein on a dry matter basis).

Effects of DL-methionine supplementation on the success of fish meal replacement by plant proteins in practical diets for juvenile gibel carp (Carassius auratus gibelio)

“…The plasma or tissue content of tested EAA has been proved to be useful in confirming dietary amino acid requirements in some fish species (Bae, Park, Yun, Hung, & Bai, ; Wilson, Harding, & Garling, ). In this study, plasma EAA concentration was not significantly affected by dietary treatments, which suggested dietary treatments had a less than 24 hr term influence on the amino acid pool, and plasma AA level was not a good indicator to determine dietary Met requirement for juvenile gibel carp.…”

Dietary valine requirement of juvenile blunt snout bream (Megalobrama amblycephalaYih, 1955)

“…Contents of free amino acids in tissues are affected by the dietary amino acid profile, and have proven to be useful to determine dietary amino acids requirements in fish such as channel catfish (Wilson et al., ) and rainbow trout (Bae et al., ). However, in the present study the plasma valine level increased with an increasing dietary valine level, suggesting that the plasma valine level was not a good indicator to determine the valine requirement in this fish species.…”

Dietary Valine Requirement of FingerlingCatla catla

“…Valine requirements have been worked out for various fish species such as fingerling chinook salmon, Oncorhynchus tshawytscha (Chance et al 1964); fingerling channel catfish, Ictalurus punctatus (Wilson et al 1980); young Nile tilapia Oreochromis niloticus (Santiago & Lovell 1988); juvenile rainbow trout, O. mykiss (Rodehutscord et al 1997); fingerling rohu, Labeo rohita (Murthy & Varghese 1997;Abidi & Khan 2004); juvenile red sea bream, Pagrus major (Forster & Ogata 1998); fry Atlantic salmon, Salmo salar (Rollin 1999); fingerling mrigal, Cirrhinus mrigala (Benakappa & Varghese 2003, Ahmed & Khan 2006; adult rainbow trout, O. mykiss (Bae et al 2012); juvenile Jian carp, Cyprinus carpio (Dong et al 2013); and juvenile red sea bream, Pagrus major (Rahimnejad & Lee 2013).…”