The Developmental Potentiality of the Liver-Rna-Treated Posterior Primitive Streak in the Chick Embryo

Niu, M. C.; Leikola, Anto

doi:10.2307/1539627

Cited by 9 publications

References 8 publications

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Studies on cell differentiation: Inducing capacity of sulfhydryl‐containing amino acids on post‐nodal pieces of chick blastoderms

Lee

Kalmus

1975

J. Exp. Zool.

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The inducing capacity of sulfhydryl (SH)-containing amino acids (cysteine and glutathione) on post-nodal pieces (PNPs) of stage 4 chick blastoderms was investigated. PNPs were treated for different lengths of time with chick Ringer's solution (control group) or chick Ringer's solution containing cysteine or glutathione, followed by culturing for 2-10 days on Spratt-Haas agar medium or on the chorioallantoic membrane of 8-day chick embryos. Control PNPs rarely showed differentiation, but those treated with the amino acids for six hours or longer developed structures such as neural tissue, notochord, somite mesoderm, and nephric tubules. The pulsatile tissue was only seen in the PNPs cultured for four days or longer. Two-four hours of treatment was too short to provoke induction in a statistically significant number of PNPs. The highest frequency of induction was noted in those pretreated with glutathione (8 mug/ml) for eight hours, followed by culturing for four days. The magnitude of the inducing capacity and toxicity of the amino acids were concentration dependent: a deleterious effect was observed at 14 mug/ml; the highest frequency of induction occurred at 8 mug/ml, but the frequency decreased as the concentration decreased; at 2 mug/ml all PNPs remained viable, but only a few (9-14%) showed differentiation. The inducing capacity of the amino acids was counteracted by equimolar concentrations of rho-chloromercuribenzoic acid or omega-chloroacetophenone. The effects of glutathione (8 mug/ml) differed from those of Hensen's node grafts in that the former caused sublethal cytolysis and inhibited H-3-uridine uptake in competent ectodermal cells during the first 18 hours of cultivation.

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Studies on cell differentiation: Inducing capacity of sulfhydryl‐containing amino acids on post‐nodal pieces of chick blastoderms

Lee

Kalmus

1975

J. Exp. Zool.

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Hensen's node — The ’Organizer’ of the amniote embryo

Leikola

1976

Experientia

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Effect of conditioned media on differentiation in mass cultures of chick limb bud cells

Schacter

1970

Experimental Cell Research

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The Developmental Potentiality of the Liver-Rna-Treated Posterior Primitive Streak in the Chick Embryo

Abstract: Previousexperiments have shown that RNA-treated posterior third of the primitive streak, here abbreviated to TPS, was capable of developing into highly organized kinds of tissue. The type of tissue produced varied according to the

Cited by 9 publications

References 8 publications

Studies on cell differentiation: Inducing capacity of sulfhydryl‐containing amino acids on post‐nodal pieces of chick blastoderms

Studies on cell differentiation: Inducing capacity of sulfhydryl‐containing amino acids on post‐nodal pieces of chick blastoderms

Hensen's node — The ’Organizer’ of the amniote embryo

Effect of conditioned media on differentiation in mass cultures of chick limb bud cells

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