Effect of conditioned media on differentiation in mass cultures of chick limb bud cells

Schacter, Lee

doi:10.1016/0014-4827(70)90327-7

Cited by 37 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tissue culture techniques have provided an opportunity to observe how various factors influence phenotypic expression (8), including the effects of teratogenic compounds that interfere with the expression of normal chondrogenic or myogenic characteristics (6,7,9,10). The activity of one such group of teratogens is completely neutralized by the coadministration of nicotinamide, and it has been postulated that variations in the concentration of nicotinamide or pyridine nucleotides within the embryonic tissue may be an important factor in the determination of phenotypic expression of mesenchymal cells.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Control of Chondrogenic Expression in Mesodermal Cells of Embryonic Chick Limb

Caplan¹,

Stoolmiller

1973

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Chick-limb mesodermal cells have been maintained in tissue culture under conditions that permit development of muscle and cartilage. 3-Acetylpyridine, a nicotinamide-antagonized muscle teratogen, potentiates chondrogenic expression in cell cultures, as evidenced by histological and biochemical criteria, including the production of chondromucoprotein. Xylosyltransferase and N-acetylgalactosaminyltransferase are two enzymes required for chondromucoprotein synthesis; the specific activities of these enzymes were measured in differentiating mesodermal cells cultured for various periods of time in the presence and absence of 3-acetylpyridine and in intact limb tissue. The ratios of specific enzyme activities were nearly the same in both cultured cells and limb tissue, although the levels of both transferases increased severalfold during chondrogenic expression by mesodermal cells in culture. 3-Acetylpyridine causes the specific activities of xylosyl-and N-acetylgalactosaminyltransferases to increase 10-and 2-fold, respectively, above those of untreated cultures. Compared to the ratio of transferase activities in limb tissue and differentiating cell cultures, 3-acetylpyridine appears to increase the activity of xylosyltransferase, the initiator of chondroitin sulfate chain synthesis, more than does N-acetylgalactosaminyltransferase; this finding implies that the synthesis of these two enzymes may be separately regulated.

show abstract

mentioning

confidence: 99%

“…Mesodermal cells can be obtained readily from chick-limb buds before stage 25 and maintained in tissue culture (6)(7)(8).…”

mentioning

confidence: 99%