The development of real-time PCR micro device with optical sensor for early detection of cancer

Kimura, Yusuke; Osada, Keigo; Inoue, Yasuhiro; Ikeuchi, Masashi; Ikuta, Koji

doi:10.1109/embc.2016.7592030

Cited by 2 publications

(3 citation statements)

References 9 publications

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“…One of the challenges of LOCs is the heating and cooling cycles between two or three discrete temperature values. Most of the conventional LOCs have an embedded electric heater on the channel surface (Morganti et al , 2011; Kimura et al ., 2016). These types of heaters have slow and nonuniform thermal cycles .More recently, efforts have been made to realize the LOCs based on plasmonic photothermal heating of gold nanoparticles (Roche et al , 2017; Kim et al .,2017; Walsh et al , 2015); but in this method, the nanoparticle-based sample preparation is not suitable for point of care (POC) testing.…”

Section: Introductionmentioning

confidence: 99%

“…When SPs are excited under resonance condition, a part of the intercepted light is dissipated through nonradiative damping, resulting in a dramatic rise in temperature in the nanometer-scale vicinity of the particle surface. This nanoscale heat has been of great interest for applications in biomedicine (Kim and Lee, 2019), where photothermal cancer therapy (Vines et al, 2019;Grosges et al, 2018), laser-induced drug release (Yu et al, 2016), nanoparticle-enhanced bioimaging (Cheng et al, 2015;Selvan et al, 2010), LOCs and mPCRs (Roche et al, 2017;Roux et al, 2014;Morganti et al, 2011;Kim et al, 2017;Kimura et al, 2016;Walsh et al, 2015;Son et al, 2016) and solar energy (Neumann et al, 2013;Woolf et al, 2018;Zhou et al, 2016), all rely on this property.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is reasonable to choose mode 1 as the RI sensing peak and can theoretically reach the sensitivity of 512 nm/RIU and FOM of 85 RIU À1 .Quality factor (Q-factor) is another parameter that determines the width and strength of a resonance. The corresponding Q-factors based on the FWHM [defined as l res / FWHM(Ru and Etchegoin, 2009;Chen, 2016)] for the modes 1 and 2 resonances in different analyte refractive indices are shown in Figures…”

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confidence: 99%

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Design and analysis of a plasmonic nanostructure applicable for heating and sensing cycles of lab-on-chip

Asgharian

Yadipour²,

Kiani³

et al. 2022

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Purpose The purpose of this study is to design a plasmonic structure that can be used simultaneously as a heater and a refractive index sensor applicable for heating and sensing cycles of lab-on-chip (LOC). Design/methodology/approach The authors report on the full optical method applicable in the heating and sensing cycles of LOC based on the plasmonic nanostructure. The novelty of this proposed structure is due to the fact that a structure simultaneously acts as a heater and a sensor. Findings In terms of the performance of the proposed structure as an analyte detection sensor, in addition to the real-time measurement, there is no need to labeling the sample. In terms of the performance of the proposed structure as a plasmonic heater, the uniformity and speed of the heating and cooling cycles have been greatly improved. Also, there is no need for experts and laboratory conditions; therefore, our proposed method can meet the conditions of point of care testing. Originality/value The authors confirm that this work is original and has not been published elsewhere nor it is currently under consideration for publication elsewhere.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Design and analysis of a plasmonic nanostructure applicable for heating and sensing cycles of lab-on-chip

Asgharian

Yadipour²,

Kiani³

et al. 2022

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3D microdevices that perform sample purification and multiplex qRT-PCR for early cancer detection with confirmation of specific RNAs

Kimura

Ikeuchi

Inoue

et al. 2018

Sci Rep

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MicroRNA expression analysis is an important screening tool for the early detection of cancer. In this study, we developed two portable three-dimensional microdevices for multiple singleplex RNA expression analysis by microRNA purification and qRT-PCR as a prototype for point-of-care testing. These microdevices are composed of several types of modules termed ‘chemical IC chips’. We successfully reduced the heating area and fluorescence observation area, reduced the energy required for the reaction, and improved the portability of all systems in the devices. The purification microdevice could purify the microRNA from the sample using the FTA elute card system. The disposable reactor module mounted on both devices was easily fabricated by deforming a 100-μm-thick polypropylene film using an uncomplicated procedure. The qRT-PCR microdevice could perform reactions for samples of small volume. We purified microRNA from the HepG2 liver cancer cell line using the purification microdevice and confirmed the expression level of miR-224, which is a potential biomarker for liver cancer. Furthermore, we observed an increase in the fluorescence intensity when we performed qRT-PCR in the qRT-PCR microdevice. Therefore, the two developed microdevices show promise as a new portable tool for early cancer detection.

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The development of real-time PCR micro device with optical sensor for early detection of cancer

Cited by 2 publications

References 9 publications

Design and analysis of a plasmonic nanostructure applicable for heating and sensing cycles of lab-on-chip

Design and analysis of a plasmonic nanostructure applicable for heating and sensing cycles of lab-on-chip

3D microdevices that perform sample purification and multiplex qRT-PCR for early cancer detection with confirmation of specific RNAs

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