3D microdevices that perform sample purification and multiplex qRT-PCR for early cancer detection with confirmation of specific RNAs

Abstract

MicroRNA expression analysis is an important screening tool for the early detection of cancer. In this study, we developed two portable three-dimensional microdevices for multiple singleplex RNA expression analysis by microRNA purification and qRT-PCR as a prototype for point-of-care testing. These microdevices are composed of several types of modules termed ‘chemical IC chips’. We successfully reduced the heating area and fluorescence observation area, reduced the energy required for the reaction, and improved the portability of all systems in the devices. The purification microdevice could purify the microRNA from the sample using the FTA elute card system. The disposable reactor module mounted on both devices was easily fabricated by deforming a 100-μm-thick polypropylene film using an uncomplicated procedure. The qRT-PCR microdevice could perform reactions for samples of small volume. We purified microRNA from the HepG2 liver cancer cell line using the purification microdevice and confirmed the expression level of miR-224, which is a potential biomarker for liver cancer. Furthermore, we observed an increase in the fluorescence intensity when we performed qRT-PCR in the qRT-PCR microdevice. Therefore, the two developed microdevices show promise as a new portable tool for early cancer detection.

Development of non-electrically controlled SalivaDirect LAMP (NEC-SD-LAMP), a new nonelectrical infectious disease testing method

“…Temperature measurements during exothermic reaction and LAMP reaction by palmitic acid were performed using a previously developed temperature control system 29 . Each temperature in the system was determined by measuring the resistance of a thermistor (104 JT; SEMITEC, Tokyo, Japan) at 50 Hz and performing calculations on a laptop.…”

Bioengineered Liver Models for Investigating Disease Pathogenesis and Regenerative Medicine

“…216 Incorporation of these beneficial properties in microfluidic organ-on-a-chip devices enabled the investigation of additional characteristics of cancer such as angiogenesis, migration, metastasis, extravasation, tumor mechanics, and drug delivery and response [216][217][218][219] ; furthermore, microfluidic devices have found utility in cancer diagnostics. [220][221][222] For example, a microfluidic device was used to culture a liver cancer cell line under mechanical confinement to promote the migration Fig. 4 In vitro models and detection of liver cancer.…”

Prospects and Opportunities for Microsystems and Microfluidic Devices in the Field of Otorhinolaryngology

“…Variants of microfluidic and microfabrication approaches have been instrumental for sequencing human genome and analyzing biomarkers of diseases. Therefore, microfluidic devices can be used for point-of-care testing and on-time diagnosis of several diseases [38][39][40][41][42][43].…”