The detection and identification of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonance

Abstract

The detection and identifi cation of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonanceThe detection of dengue virus (DENV) serotypes has been described here, based on the distance dependent localized surface plasmon resonance (LSPR) between CdSeTeS QDs and AuNPs, which generates an altered fl uorescence signal for each serotype of DENV. In the reaction mixture of hairpin nanoprobes and functionalized AuNPs, the addition of analyte oligos of DENV can open the hairpin structure of the nanoprobe to form various structures depending on the serotypes initiating the LSPR enhancement or the quenching eff ect on the QD fl uorescence.Registered charity number: 207890 rsc.li/nanoscale-advancesThe detection and identification of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonance † The dengue hemorrhagic fever or dengue shock syndrome has become a severe human fatal disease caused by infection with one of the four closely related but serologically distinct dengue viruses (DENVs).All four dengue serotypes are currently co-circulating throughout the subtropics and tropics. Since the fatality rate increases severely when a secondary infection occurs by a virus serotype different from that of the initial infection, serotype identification is equally important as virus detection. In this study, the development and validation of a rapid and quantitative DENV serotype-specific (serotypes 1-4) biosensor are reported by optimizing the stable system between cadmium selenide tellurium sulphide fluorescent quantum dots (CdSeTeS QDs) and gold nanoparticles (AuNPs). Four different nanoprobes are designed using each primer-probe serotype-specific hairpin single-stranded DNA covalently bound at different positions to CdSeTeS QDs, which generates an altered fluorescence signal for each serotype of DENV. In fourplex reactions with free functionalized AuNPs and the four nanoprobes, the standard dilutions of the target virus DNA from 10 À15 to 10 À10 M were successfully detected. The limit of detection was found to be in the femtomolar range for all four serotypes, where the serotype detection ability was undoubtedly established. To confirm the applicability of this sensing performance in long chained complex RNAs, the sensor was also applied successfully to RNAs extracted from DENV culture fluids for serotype identification as well as quantification, which can lead to a potential diagnostic probe for point-of-care detection. Fig. 6 (A-D) Concentration-dependent fluorescence changes of the CdSeTeS-dsDNA-AuNP nanocomposites in the presence of the target analyte DENV ssDNA of all four serotypes in the concentration range of 10 À15 to 10 À9 M. (E) Calibration curves for all four DENV serotypes.This journal is

Point-of-care diagnostics for infectious diseases: From methods to devices

“…In the presence of the complementary RNA of DENV, the hairpin structure of the nanoprobe would be opened due to the better hybridization of the nanoprobe ssDNA and complementary RNA of DENV in its loop region, and poly-C-functionalized AuNPs would then complementary bind with the poly-G region of the nanoprobe, resulting in the LSPR enhancement (at around 14 nm) or quenching (at around 3 nm) effect on the fluorescence of the QDs, which was determined by the distance between the QDs and the AuNPs. After optimization, the SPR-based biosensor could achieve the detection of standard dilutions of the target virus DNA from 1 × 10 −9 to 1 × 10 −4 μM with a detection limit of femtomolar range, demonstrating a great potential for POC detection of dengue virus [249] . Moreover, a portable, multiplex, inexpensive microfluidic-integrated SPR platform was developed for rapidly POC detection and quantification of E. coli ( Fig.…”

“…Reproduced with permission from Ref. [249] Copyright 2020, the Royal Society of Chemistry. (C) Schematic of Portable plasmonic platform for pathogen detection and quantification.…”

Discussions of Fluorescence in Selenium Chemistry: Recently Reported Probes, Particles, and a Clearer Biological Knowledge

“…The formation of AuNP–dsDNA–CdSeTeS was reported. The general approach, considering the virus reagent including and aside from recent research interest in COVID 19 virus detection, is a hot topic in virus research because of the current number of people affected by the 2019–2021 pandemic [ 138 ].…”

Biosensors Based on Advanced Sulfur-Containing Nanomaterials

“…Rong et al [ 142 ] have synthesized novel Eu 3+ ion-functionalized fluorescent MoS 2 quantum dots for biosensing Guanosine 3′-diphosphate-5′-diphophate (ppGpp) ( Figure 5 A). Literature has also reported fluorescence biosensors for determining other biomolecules, such as thrombin, glutathione S-transferase enzyme, bilirubin [ 91 , 94 , 143 , 144 ] via PL strategies.…”