The dentin sialoprotein (DSP) expression in rat tooth germs following fluoride treatment: An immunohistochemical study

Maciejewska, Izabela; Spodnik, Jan Henryk; Wójcik, Sławomir; Domaradzka-Pytel, B; Bereznowski, Zdzisław

doi:10.1016/j.archoralbio.2005.07.003

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…Therefore, it seems unlikely that a 21% reduction in MMP-20 expression would be the major factor responsible for the clinical manifestations observed in enamel fluorosis. Immunohistochemical analysis of DSP protein in the first molar tooth germ showed that fluoride not only increased, but also prolonged the expression of this protein in ameloblasts of fluoride-treated animals [24]. Fluoride also increased the expression of DSPP mRNA, a marker of early ameloblast differentiation [25], in the early stages of ameloblast differentiation [26].…”

Section: Discussionmentioning

confidence: 96%

Fluoride effect on the secretory-stage enamel organic extracellular matrix of mice

Mofatto

Frozoni

Santo

et al. 2010

Connective Tissue Research

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The formation of an ordered enamel organic extracellular matrix (EOECM) seems to be a crucial step for the proper formation of the enamel mineral phase. The ordered supramolecular structure of the EOECM in the secretory stage can be analyzed using polarizing microscopy, as it is strongly birefringent. Excessive fluoride (F) ingestion during tooth development can cause enamel fluorosis, leading to increased porosity in mature enamel. We analyzed the effects of F on the birefringence of the EOECM in the A/J, CBA, and DBA/2 strains of mice given 0, 11.25, and 45 ppm of fluoride in drinking water. In the CBA and DBA/2 strains, the 11.25 and 45 ppmF groups presented a significant decrease in optical retardation (OR) when compared with the respective 0 (CBA 11.25 ppmF p = 0.0056 and 45 ppmF p < 0.0001; DBA/2 11.25 and 45 ppmF p < 0.05). ORs in A/J 0 ppmF were significantly higher than in 45 (p < 0.0001). The enamel of the A/J strain was more severely affected by fluoride than it was in the other strains of mice and exhibited the lowest levels of fluoride in plasma, whereas its normal secretory enamel presented a significantly higher protein absorbance than it did in CBA and DBA mice (p = 0.0099 and p = 0.0025, respectively). The results showed that experimental fluorosis can alter the supramolecular organization of EOECM in the secretory stage of amelogenesis and that the susceptibility to dental fluorosis seems to be influenced by the inherent characteristics of the developing enamel.

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Section: Discussionmentioning

confidence: 96%

Fluoride effect on the secretory-stage enamel organic extracellular matrix of mice

Mofatto

Frozoni

Santo

et al. 2010

Connective Tissue Research

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“…However, we did find DSPP, a marker of early ameloblast differentiation (MacDougall et al, 1998), down-regulated in the youngest cell group, isolated from the developing 2nd primary molars. The recent report by Maciejewska and coworkers (Maciejewska et al, 2006), showing variable fluoride effects on DSP protein in vivo, suggests that further studies of fluoride on DSPP expression at the early stages of ameloblast differentiation are warranted.…”

Section: Discussionmentioning

confidence: 99%

Micromolar Fluoride Alters Ameloblast Lineage Cells in vitro

Yan

Zhang

et al. 2007

J Dent Res

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Fluorosed enamel is caused by exposure to fluoride during tooth formation. The objective of this study was to determine whether epithelial ameloblast-lineage cells, derived from the human enamel organ, are directly affected by micromolar concentrations of fluoride. Cells were cultured in the presence of fluoride, and proliferation was measured by BrdU incorporation. The effect of 0, 10, or 20 microM fluoride on apoptosis was determined by the flow cytometry apoptotic index. The effects of fluoride on gene expression were investigated by SuperArray microarray analysis and real-time PCR. Fluoride had a biphasic effect on cell proliferation, with enhanced proliferation at 16 microM, and reduced proliferation at greater than 1 mM F. Flow cytometry showed that both 10 microM and 20 microM NaF significantly increased the apoptotic index of ameloblast-lineage cells. There was no general effect of fluoride on gene expression. These results indicate multiple effects of micromolar fluoride on ameloblast-lineage cells.

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“…2004). Work by Maciejewska et al. (2006) revealed that fluoride changed the expression pattern of DSP protein in rat germs, and real‐time PCR analysis has demonstrated that DSPP mRNA expression was consistently down‐regulated by fluoride exposure in the youngest ameloblasts harvested from second primary molars (Yan et al.…”

Section: Discussionmentioning

confidence: 99%