Gene expression profiles of the incisor pulp tissue during fluorosis

Wu, Yafei; Hao, Yuqing; Li, J.-Y.; Zhou, Xuedong

doi:10.1111/j.1365-2591.2010.01697.x

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…Although Wurtz et al [20] demonstrated 8 differentially expressed genes in odontoblast culture cells induced by a fluoride at a non-toxic dose (1 mM), these were not detected in genes that were identified in our cell system. Wu et al [21] also reported that 247 genes were 1.5-fold or more differentially expressed after fluoride treatment in rat incisor pulp tissue. Up-regulation of period circadian clock 1 ( Per1 ) and down-regulations of calpastatin ( Cast ) and programmed cell death 4 ( Pdcd4 ) could be detected in ROE2 cells treated with NaF.…”

Section: Discussionmentioning

confidence: 99%

“…Transcript profiling technologies including DNA microarray have made it possible to profile global gene expression patterns associated with different biological responses during chemical and physiological stresses. To date, several studies have utilized this technology for identifying changes in the gene expression of odontoblast culture cells induced by a fluoride at non-toxic doses [20], incisor pulp tissue during fluorosis [21] and the sperm of mice treated with fluoride [22]. Recently, pathway analysis technologies have enabled the mapping of gene expression data into relevant pathway maps on the basis of their functional annotation and known molecular interactions and we have used these in biological experiments [23,24].…”

Section: Introductionmentioning

confidence: 99%

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Genes and Gene Networks Involved in Sodium Fluoride-Elicited Cell Death Accompanying Endoplasmic Reticulum Stress in Oral Epithelial Cells

Tabuchi

Yunoki

Hoshi

et al. 2014

IJMS

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Here, to understand the molecular mechanisms underlying cell death induced by sodium fluoride (NaF), we analyzed gene expression patterns in rat oral epithelial ROE2 cells exposed to NaF using global-scale microarrays and bioinformatics tools. A relatively high concentration of NaF (2 mM) induced cell death concomitant with decreases in mitochondrial membrane potential, chromatin condensation and caspase-3 activation. Using 980 probe sets, we identified 432 up-regulated and 548 down-regulated genes, that were differentially expressed by >2.5-fold in the cells treated with 2 mM of NaF and categorized them into 4 groups by K-means clustering. Ingenuity® pathway analysis revealed several gene networks from gene clusters. The gene networks Up-I and Up-II included many up-regulated genes that were mainly associated with the biological function of induction or prevention of cell death, respectively, such as Atf3, Ddit3 and Fos (for Up-I) and Atf4 and Hspa5 (for Up-II). Interestingly, knockdown of Ddit3 and Hspa5 significantly increased and decreased the number of viable cells, respectively. Moreover, several endoplasmic reticulum (ER) stress-related genes including, Ddit3, Atf4 and Hapa5, were observed in these gene networks. These findings will provide further insight into the molecular mechanisms of NaF-induced cell death accompanying ER stress in oral epithelial cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genes and Gene Networks Involved in Sodium Fluoride-Elicited Cell Death Accompanying Endoplasmic Reticulum Stress in Oral Epithelial Cells

Tabuchi

Yunoki

Hoshi

et al. 2014

IJMS

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“…From Wu et al . (2010) – ‘Sixteen female wistar rats weighing approximately 100g were obtained from Experimental Animal Center of Guangxi Medical University. This study was approved by the animal care and use committee of Guangxi Medical University.…”

Section: Introductionmentioning

confidence: 99%

PRIASE 2021 guidelines for reporting animal studies in Endodontology: explanation and elaboration

et al. 2021

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Laws and ethics require that before conducting human clinical trials, a new material, device or drug may have to undergo testing in animals in order to minimize health risks to humans, unless suitable supporting grandfather data already exist. The Preferred Reporting Items for Animal Studies in Endodontology (PRIASE) 2021 guidelines were developed exclusively for the specialty of Endodontology by integrating and adapting the ARRIVE (Animals in Research: Reporting In Vivo Experiments) guidelines and the Clinical and Laboratory Images in Publications (CLIP) principles using a validated consensus‐based methodology. Implementation of the PRIASE 2021 guidelines will reduce potential sources of bias and thus improve the quality, accuracy, reproducibility, completeness and transparency of reports describing animal studies in Endodontology. The PRIASE 2021 guidelines consist of a checklist with 11 domains and 43 individual items and a flowchart. The aim of the current document is to provide an explanation for each item in the PRIASE 2021 checklist and flowchart and is supplemented with examples from the literature in order for readers to understand their significance and to provide usage guidance. A link to the PRIASE 2021 explanation and elaboration document and PRIASE 2021 checklist and flowchart is available on the Preferred Reporting Items for study Designs in Endodontology (PRIDE) website (http://pride-endodonticguidelines.org/priase/).

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“…The following studies were identified for fluoride (Ge et al, 2011;Sun et al, 2011;Wu et al, 2010;Wurtz et al, 2008;Yan et al, 2007). Four used transcriptomics approaches and one proteomics.…”

Section: Hazardmentioning

confidence: 99%

A foresight study on emerging technologies: State of the art of Omics technologies and potential applications in food and feed safety

Pielaat

Barker

Hendriksen

et al. 2013

EFSA Supporting Publications

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Suggested citation: Pielaat A, Barker GC, Hendriksen P, Peijnenburg A and Kuile BH, 2013. A foresight study on emerging technologies: State of the art of omics technologies and potential applications in food and feed safety. REPORT 1: Review on the state of art of omics technologies in risk assessment related to food and feed safety. EFSA supporting publication 2013:EN-495, 126 pp. ABSTRACTThis report consists of a review of the available scientific evidence on the state of art of omics technologies with a particular focus on applications in the area of food and feed safety. The report gives a description of the different types of omics technologies with respect to recent progresses made on the application of these techniques in chemical and microbiological risk assessment. Hereto, case studies were selected in order to cover different areas under the remit of EFSA. In this review each case study has been investigated with respect to its hazard, experimental methodologies, (statistical) data analysis and its link to food/feed safety risk assessment. Finally, the added value of omics technologies as compared to classical risk assessment and current limitations of omics technologies for their application in chemical and microbiological food safety risk assessment has been discussed. This investigation shows that most of the studies on chemical agents focus on mode of action identification and a search for biomarkers using transcriptomics. As for microbial food safety, omics have not yet been successfully applied often. When applied, they never target the host, but focus on aspects of the pathogen, e.g. in detection or source attribution.

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Gene expression profiles of the incisor pulp tissue during fluorosis

Abstract: The gene expression levels in rat pulp tissue changed during fluorosis. The gene expression profiles between the fluorotic model and the normal group will help to understand the multiple pathogenic mechanisms for the altered mineralization patterns observed in fluorotic dentine.

Cited by 6 publications

References 40 publications

Genes and Gene Networks Involved in Sodium Fluoride-Elicited Cell Death Accompanying Endoplasmic Reticulum Stress in Oral Epithelial Cells

Genes and Gene Networks Involved in Sodium Fluoride-Elicited Cell Death Accompanying Endoplasmic Reticulum Stress in Oral Epithelial Cells

PRIASE 2021 guidelines for reporting animal studies in Endodontology: explanation and elaboration

A foresight study on emerging technologies: State of the art of Omics technologies and potential applications in food and feed safety

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