Micromolar Fluoride Alters Ameloblast Lineage Cells <i>in vitro</i>

Yan, Qiaomei; Zhang, Y.; Li, W.; DenBesten, Pamela

doi:10.1177/154405910708600407

Cited by 54 publications

(47 citation statements)

References 24 publications

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“…The impact of fluoride on ALP activity is still controversial and it seems to be dependent on the experimental design. Both up and down modulation have been found after fluoride treatment using osteoblasts (15)(16)(17). In the present study, there were no differences among the control and treated preosteoblasts.…”

Section: Discussionmentioning

confidence: 43%

“…* p<0.05; *** p<0.01. (17,21). In addition, other authors showed that fluoride does not induce DNA damage (22).…”

Section: Discussionmentioning

confidence: 99%

“…The effect of fluoride on proliferation has also been shown in other cell types. In this context, Yan et al (17) showed that NaF at doses of 4, 16 and 64 µM led to increased ameloblast proliferation, while the dose of 1,024 µM promoted apoptosis impairing osteoblast survival. The obtained results corroborate others, showing that fluoride affects cell proliferation in a dose-dependent fashion.…”

Section: Discussionmentioning

confidence: 99%

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Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

et al. 2012

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This study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10 -6 M, 10 -5 M, 10 -4 M and 10 -3 M) for 24, 48, 72 and 96 h at 37 o C, 5% CO 2 . Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10 -3 M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling.

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Section: Discussionmentioning

confidence: 43%

“…* p<0.05; *** p<0.01. (17,21). In addition, other authors showed that fluoride does not induce DNA damage (22).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

et al. 2012

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“…The following studies were identified for fluoride (Ge et al, 2011;Sun et al, 2011;Wu et al, 2010;Wurtz et al, 2008;Yan et al, 2007). Four used transcriptomics approaches and one proteomics.…”

Section: Hazardmentioning

confidence: 99%

“…The following studies were identified for xenobiotic response (Skipsey et al, 2011;Smith et al, 2004;Zhang et al, 2007). Safeners coordinately induce the expression of multiple proteins and MRP transcripts involved in herbicide metabolism and detoxification in Triticum tauschii seedling tissues.…”

mentioning

confidence: 99%

A foresight study on emerging technologies: State of the art of Omics technologies and potential applications in food and feed safety

Pielaat

Barker

Hendriksen

et al. 2013

EFSA Supporting Publications

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Suggested citation: Pielaat A, Barker GC, Hendriksen P, Peijnenburg A and Kuile BH, 2013. A foresight study on emerging technologies: State of the art of omics technologies and potential applications in food and feed safety. REPORT 1: Review on the state of art of omics technologies in risk assessment related to food and feed safety. EFSA supporting publication 2013:EN-495, 126 pp. ABSTRACTThis report consists of a review of the available scientific evidence on the state of art of omics technologies with a particular focus on applications in the area of food and feed safety. The report gives a description of the different types of omics technologies with respect to recent progresses made on the application of these techniques in chemical and microbiological risk assessment. Hereto, case studies were selected in order to cover different areas under the remit of EFSA. In this review each case study has been investigated with respect to its hazard, experimental methodologies, (statistical) data analysis and its link to food/feed safety risk assessment. Finally, the added value of omics technologies as compared to classical risk assessment and current limitations of omics technologies for their application in chemical and microbiological food safety risk assessment has been discussed. This investigation shows that most of the studies on chemical agents focus on mode of action identification and a search for biomarkers using transcriptomics. As for microbial food safety, omics have not yet been successfully applied often. When applied, they never target the host, but focus on aspects of the pathogen, e.g. in detection or source attribution.

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Toxicity of fluoride: critical evaluation of evidence for human developmental neurotoxicity in epidemiological studies, animal experiments and in vitro analyses

et al. 2020

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Recently, epidemiological studies have suggested that fluoride is a human developmental neurotoxicant that reduces measures of intelligence in children, placing it into the same category as toxic metals (lead, methylmercury, arsenic) and polychlorinated biphenyls. If true, this assessment would be highly relevant considering the widespread fluoridation of drinking water and the worldwide use of fluoride in oral hygiene products such as toothpaste. To gain a deeper understanding of these assertions, we reviewed the levels of human exposure, as well as results from animal experiments, particularly focusing on developmental toxicity, and the molecular mechanisms by which fluoride can cause adverse effects. Moreover, in vitro studies investigating fluoride in neuronal cells and precursor/stem cells were analyzed, and 23 epidemiological studies published since 2012 were considered. The results show that the margin of exposure (MoE) between no observed adverse effect levels (NOAELs) in animal studies and the current adequate intake (AI) of fluoride (50 µg/kg b.w./day) in humans ranges between 50 and 210, depending on the specific animal experiment used as reference. Even for unusually high fluoride exposure levels, an MoE of at least ten was obtained. Furthermore, concentrations of fluoride in human plasma are much lower than fluoride concentrations, causing effects in cell cultures. In contrast, 21 of 23 recent epidemiological studies report an association between high fluoride exposure and reduced intelligence. The discrepancy between experimental and epidemiological evidence may be reconciled with deficiencies inherent in most of these epidemiological studies on a putative association between fluoride and intelligence, especially with respect to adequate consideration of potential confounding factors, e.g., socioeconomic status, residence, breast feeding, low birth weight, maternal intelligence, and exposure to other neurotoxic chemicals. In conclusion, based on the totality of currently available scientific evidence, the present review does not support the presumption that fluoride should be assessed as a human developmental neurotoxicant at the current exposure levels in Europe.

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Micromolar Fluoride Alters Ameloblast Lineage Cells in vitro

Cited by 54 publications

References 24 publications

Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities

A foresight study on emerging technologies: State of the art of Omics technologies and potential applications in food and feed safety

Toxicity of fluoride: critical evaluation of evidence for human developmental neurotoxicity in epidemiological studies, animal experiments and in vitro analyses

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