The degradation of proteins in pinniped skeletal muscle: viability of post-mortem tissue in physiological research

Moore, Colby D.; Fahlman, Andreas; Crocker, Daniel E.; Robbins, Kathleen A.; Trumble, Stephen J.

doi:10.1093/conphys/cov019

Cited by 4 publications

(1 citation statement)

References 53 publications

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“…In addition, although in this case the tissue was collected within 12 h, the timing of tissue collection is of critical importance and might affect the microanatomical structure. A previous study reported that enzymatic activity fluctuates greatly in the first 48 h, depending on storage temperature ( Moore et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Histology of 24 organs from Asian elephant calves (Elephas maximus)

Thitaram

Matchimakul

Pongkan

et al. 2018

PeerJ

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BackgroundElephants are the largest and heaviest living terrestrial animals, but information on their histology is still lacking. This study provides a unique insight into the elephant’s organs and also provides a comparison between juvenile Asian elephants and adult Asian elephants or other species. Here we report on the histological structure of 24 organs, including the skin, brain (cerebrum, cerebellar hemisphere, vermis, thalamus, midbrain), spinal cord, sciatic nerve, striated skeletal muscle, cardiac muscle, bone (flat bone and long bone), cartilage (hyaline cartilage and fibrocartilage), heart (right atrium, right ventricle), blood vessels (aorta, pulmonary artery and caudal vena cava), trunk, trachea, lung, tongue, esophagus, stomach, small intestine (duodenum, jejunum, ileum), large intestine (cecum, colon, rectum), liver and pancreas, kidney, ovary, uterus (body and horn) and spleen of two juvenile Asian elephants.MethodsTissue sections were stained with Harris’s hematoxylin and eosin Y.ResultsWhile almost all structures were similar to those of other species or adult elephants, some structures were different from other mammalian species, such as: plexiform bone was found in flat bone only; a thin trachealismuscle was observed in the trachea; and no serous or mucinous glands were found in the submucosa of the trachea.DiscussionHistological information from various organs can serve as an important foundation of basal data for future microanatomical studies, and help in the diagnosis and pathogenesis in sick elephants or those with an unknown cause of death.

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Section: Discussionmentioning

confidence: 99%

Histology of 24 organs from Asian elephant calves (Elephas maximus)

Thitaram

Matchimakul

Pongkan

et al. 2018

PeerJ

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Elements and antioxidants in wild boar from northwestern Russia

et al. 2022

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Best practices for collecting and preserving marine mammal biological samples in the ‘omics era

Van Cise,

Switzer,

Apprill

et al. 2024

Marine Mammal Science

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The recent rise of ‘omics and other molecular research technologies alongside improved techniques for tissue preservation have broadened the scope of marine mammal research. Collecting biological samples from wild marine mammals is both logistically challenging and expensive. To enhance the power of marine mammal research, great effort has been made in both the field and the laboratory to ensure the scientific integrity of samples from collection through processing, supporting the long‐term use of precious samples across a broad range of studies. However, identifying the best methods of sample preservation can be challenging, especially as this technological toolkit continues to evolve and expand. Standardizing best practices could maximize the scientific value of biological samples, foster multi‐institutional collaborative efforts across fields, and improve the quality of individual studies by removing potential sources of error from the collection, handling, and preservation processes. With these aims in mind, we summarize relevant literature, share current expert knowledge, and suggest best practices for sample collection and preservation. This manuscript is intended as a reference resource for scientists interested in exploring collaborative studies and preserving samples in a suitable manner for a broad spectrum of analyses, emphasizing support for ‘omics technologies.

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The degradation of proteins in pinniped skeletal muscle: viability of post-mortem tissue in physiological research

Cited by 4 publications

References 53 publications

Histology of 24 organs from Asian elephant calves (Elephas maximus)

Histology of 24 organs from Asian elephant calves (Elephas maximus)

Elements and antioxidants in wild boar from northwestern Russia

Best practices for collecting and preserving marine mammal biological samples in the ‘omics era

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