The current role of liquid chromatography-tandem mass spectrometry in therapeutic drug monitoring of immunosuppressant and antiretroviral drugs

Taylor, Paul J.; Tai, Chun-Hui; Franklin, Michael E.; Pillans, Peter I.

doi:10.1016/j.clinbiochem.2010.06.012

Cited by 63 publications

(33 citation statements)

References 85 publications

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“…The selectivity benefit of LC-MS/MS has encouraged some laboratories to adopt this technology for tacrolimus monitoring [13]. There have been several recent reviews on the use of LC-MS/MS for the monitoring of tacrolimus and the other immunosuppressants in this class of drugs [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic drug monitoring of tacrolimus by liquid chromatography–tandem mass spectrometry: Is it truly a routine test?

Taylor

Franklin

Tai

et al. 2012

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Therapeutic drug monitoring of tacrolimus by liquid chromatography–tandem mass spectrometry: Is it truly a routine test?

Taylor

Franklin

Tai

et al. 2012

Journal of Chromatography B

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“…In recent years, many analytical methods have been reported for the analysis of mTOR inhibitors in biological samples. [571][572][573][574]84 Two analytical techniques can be discerned: immunoassays and LC. The comparison of these two approaches shows their comparable performances.…”

Section: Mtor Inhibitorsmentioning

confidence: 99%

Antineoplastic drugs and their analysis: a state of the art review

Guichard

Guillarme

Bonnabry

et al. 2017

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The number of patients suffering from cancer is constantly increasing and, consequently, the number of different chemotherapy treatments administered is increasing. Given the high reactivity and toxicity of antineoplastic drugs, analytical methods are required in all pharmaceutical fields, from drug development to their elimination in wastewater; including formulation quality control, environment and human exposure and therapeutic drug monitoring. The aim of this paper is to provide an overview of the analytical methods available for the determination of antineoplastic drugs in different matrices such as pharmaceutical formulations, biological and environmental samples. The applicability and performance of the reported methods will be critically discussed, with focus on the most commonly used antineoplastic drugs. Only conventional compounds and small molecules for targeted therapy will be considered in the present review.

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“…Alternate separation techniques such as HPLC, LC-MS/MS, MALDI-TOF/TOF and MALDI triple quadrupole MS/MS have been described [10][11][12][13][14][15]. However, these costly methods require highly qualified human resources and an extremely well-equipped infrastructure limiting their use in SubSaharan Africa.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of zidovudine, stavudine, lamivudine and nevirapine by Micellar Electrokinetic Capillary Chromatography

et al. 2012

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-Purpose: Recent improvements to the availability of antiretroviral therapy in Sub-Saharan Africa can be attributed to the generic formulation of antiretroviral drugs. Quality drug surveillance, routine supervision and adherence data are however restricted owing to a fundamental lack of resources in the area. Accordingly we have developed an affordable micellar electrokinetic capillary chromatography (MEKC) method for the simultaneous detection and quantification of zidovudine, stavudine, lamivudine and nevirapine in plasma. Methods: The antiretroviral drugs were extracted by solid phase extraction. Various factors influencing separation of the four drugs have been optimized. A buffer consisting of 5 mM sodium tetraborate at pH 9.8, containing 50 mM SDS, 30% methanol and 5% ethanol was found to be particularly suitable and the MEKC method was validated. Results: All validation parameters were within the 20% acceptation limit, except for the interday precision of stavudine which required a daily calibration curve. The limit of quantification (LOQ) for zidovudine, stavudine, lamivudine and nevirapine were 0.037, 0.051, 0.029 and 0.028 mg/L respectively and were below the therapeutic concentration ranges of each drug. The optimized MEKC method was successfully applied to 16 human plasma samples. Conclusion: Our sensitive and validated method was demonstrated to be suitable for simultaneous detection and quantification of zidovudine, stavudine, lamivudine and nevirapine. This cost-effective method could be of interest for resource limited countries not only for adherence or therapeutic monitoring but also for steady-state pharmacokinetic studies of generic ARV drugs.Key words: Capillary electrophoresis, drug monitoring, highly active antiretroviral therapy, Micellar Electrokinetic Capillary Chromatography, plasma Résumé -Objectif : L'accès aux thérapies antirétrovirales en Afrique sub-saharienne s'est récemment nettement amélioré grâce à l'apparition des antirétroviraux sous la forme de génériques. Cependant, la surveillance de la qualité des médicaments, le suivi thérapeutique de routine et les données concernant l'observance restent limités dans ces régions du fait du manque de ressources. Par conséquent, nous avons développé une méthode de chromatographie électrocinétique micellaire (CEM) à faible coût permettant la détection et la quantification simultanée de zidovudine, stavudine, lamivudine et névirapine dans le plasma. Méthodes : Une extraction en phase solide a été réalisée afin d'isoler les médicaments antirétroviraux. Différents facteurs influençant la séparation des quatre composants ont été optimisés. Un tampon constitué de tétraborate de sodium 5 mM à pH 9,8, contenant 50 mM de SDS, 30 % méthanol et 5 % éthanol s'avérait le plus adéquat et la méthode CEM a été validée. zidovudine, de la stavudine, de la lamivudine et de la névirapine étaient respectivement de 0,037, 0,051, 0,029 et 0,028 mg/L, par conséquent, inférieures aux concentrations thérapeutiques de chaque médicament. Seize plasmas huma...

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The current role of liquid chromatography-tandem mass spectrometry in therapeutic drug monitoring of immunosuppressant and antiretroviral drugs

Cited by 63 publications

References 85 publications

Therapeutic drug monitoring of tacrolimus by liquid chromatography–tandem mass spectrometry: Is it truly a routine test?

Therapeutic drug monitoring of tacrolimus by liquid chromatography–tandem mass spectrometry: Is it truly a routine test?

Antineoplastic drugs and their analysis: a state of the art review

Simultaneous quantification of zidovudine, stavudine, lamivudine and nevirapine by Micellar Electrokinetic Capillary Chromatography

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