Therapeutic drug monitoring of tacrolimus by liquid chromatography–tandem mass spectrometry: Is it truly a routine test?

Taylor, Paul J.; Franklin, Michael E.; Tai, Chun-Hui; Pillans, Peter I.

doi:10.1016/j.jchromb.2011.06.024

Cited by 25 publications

(18 citation statements)

References 35 publications

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“…This technique is highly sensitive and specific for the parent drug compared to immunoassays, which are sometimes associated with non-specific binding of the antibody to metabolites and endogenous compounds (10). Study results, however, should not be extrapolated beyond the patient group in which they were derived, as defined by factors such as graft type, time post-transplantation and analytical technique used for drug measurement.…”

Section: Discussionmentioning

confidence: 99%

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Population Pharmacokinetics of Tacrolimus in Adult Kidney Transplant Patients

Bergmann

Hennig

Barraclough

et al. 2014

Therapeutic Drug Monitoring

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Objectives: The aims of this study were to develop a population pharmacokinetic model of tacrolimus in adult kidney transplant recipients; to use this model to compare cytochrome P450 3A5 (CYP3A5) genotype based initial dosing of tacrolimus to standard per-kilogram based dosing; and to predict the best starting dose of tacrolimus based on patient genotype to achieve a trough concentration between 6 and10 µg/L by day 5 post-transplantation.Methods: Population analysis was performed using the software program NONMEM®. Tacrolimus

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Section: Discussionmentioning

confidence: 99%

“…Tacrolimus concentrations were determined in whole blood using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection (10,11). This assay was linear over the range 0.5 to 50 µg/L.…”

Section: Tacrolimus and Prednisolone Analysismentioning

confidence: 99%

Population Pharmacokinetics of Tacrolimus in Adult Kidney Transplant Patients

Bergmann

Hennig

Barraclough

et al. 2014

Therapeutic Drug Monitoring

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“…The technique has also been shown to be free from the many factors that negatively impact commercially available immunoassays, namely tacrolimus metabolites (13 ), hematocrit and serum albumin (14 -16 ), and heterophilic antibodies (17 ). Although LC-MS has traditionally been considered a cumbersome technique, one relegated to the research laboratory, the rapid sample pretreatment and chromatography afforded by modern LC-MS instrumentation have led Taylor et al to conclude recently that LC-MS for tacrolimus therapeutic drug monitoring truly is a routine test (18 ).…”

Section: Discussionmentioning

confidence: 99%

Standardization of LC-MS for Therapeutic Drug Monitoring of Tacrolimus

et al. 2013

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BACKGROUND: LC-MS is increasingly used for therapeutic drug monitoring of tacrolimus. A recent summary from an international proficiency-testing scheme demonstrated that the mass spectrometry respondents were the largest method group. However, these methods lack standardization, which may explain the relatively poor interlaboratory agreement for such methods. This study aimed to provide one path toward the standardization of tacrolimus quantification by use of LC-MS.

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“…Elevated TAC concentrations can cause serious toxicity and long-term morbidity, while under-dosing could lead to rejection. The individualization of TAC therapy through measured concentrations aims to achieve maximum therapeutic response with minimal adverse effects [3]. In routine clinical settings, TAC concentrations are measured in whole blood.…”

Section: Introductionmentioning

confidence: 99%