The cross-talk between matrix metalloproteinase-9, RANKL/OPG system and cardiovascular risk factors in ovariectomized rat model of postmenopausal osteoporosis

Abstract

Epidemiology and pathogenesis of cardiovascular diseases (CVD) and osteoporosis are strikingly overlapping. This study presents matrix metalloproteinase-9 (MMP-9), as a simple molecular link more consistently associated with the pathophysiology of both osteoporosis and CVD risk factors. 40 adult female rats were randomly distributed into 4 groups [control sham-operated, untreated osteoporosis, carvedilol-treated osteoporosis and alendronate-treated osteoporosis]. After 8 weeks, blood samples were collected to estimate Lipid profile (Total cholesterol, HDL, Triglycerides), inflammatory markers (IL-6, TNF alpha, CRP and NO), and Bone turnover markers (BTM) (Alkaline phosphatase, osteocalcin and pyridinoline). The tibias were dissected to estimate MMP-9 and NF-kB gene expression, OPG, RANKL levels and for histological examination. Induction of osteoporosis resulted in a significant elevation in BTM, inflammatory markers and dyslipidemia. MMP-9 was significantly elevated and positively correlated with BTM, inflammation and dyslipidemia markers. Carvedilol and alendronate exerted a bone preservative role and attenuated dyslipidaemia and inflammation in accordance with their respective effect on MMP-9.

Effect of atorvastatin on bone formation in ovariectomized rats

“…This result indicates the utility of using atorvastatin in bone formation, and atorvastatin efficiently ameliorated ovx-induced osteoporosis. This study's increase in ALP activity agrees with the result of Sabry et al (25). They noticed an elevation in the serum ALP in the ovx group.…”

The effect of Rutin hydrate on Glucocorticoids induced osteoporosis in mandibular alveolar bone in Albino rats (Radiological, histological and histochemical study)

“…This could be attributed to that GCs have the ability to inhibit prostaglandins production like prostaglandin-E2 which can normally stimulate collagen and non-collagenous proteins synthesis ( Raisz, 1999 ). Our results are in parallel with Sabry et al, 2021 who detected obvious reduction of bluish-stained collagen content in bone matrix of ovarectomized rats.…”

Study on the influencing factors of bone mass abnormalities in postmenopausal Tibetan women in Tibet

“…Hypertensive heart disease refers to the structural and functional changes of the heart caused by longterm inadequate control of hypertension, which can lead to cardiac diastolic dysfunction and eventually heart failure. Numerous studies have shown a strong association between cardiovascular disease and osteoporosis [11,12] . In the study, hypertensive heart disease was a risk factor for osteoporosis.…”