A monoclonal antibody that reacts with all known isoforms of troponin I detected a single isoform of cardiac troponin I in both atrial and ventricular chambers of adult chicken and rat hearts in an immunoblotting analysis. Another isoform of troponin I in addition to the adult cardiac form, however, was present in all chambers of the heart during early development in both species. This developmental isoform appeared to have the same electrophoretic mobility on SDS tris glycine polyacrylamide gels as that observed for the adult slow skeletal muscle isoform. In the rat, only the developmental isoform of troponin I was present in the early foetal heart and small amounts of the adult cardiac isoform were not apparent until late in gestation, whereas the developmental and adult isoforms were expressed in approximately equal amounts throughout embryonic development in the chicken. The level of developmental isoform of troponin I in both the chicken and the rat hearts gradually decreased so that only small amounts of this variant were detectable two weeks after birth or post hatching.
Using a monoclonal antibody (CDC4) that recognizes both the cardiac and slow skeletal isoforms of troponin T in an immunoblotting procedure, the composition of troponin T isoforms in adult and developing skeletal muscles of the rat and human were studied. Two major isoforms of slow troponin T (HS1 and HS2) were detected in all the adult human skeletal muscles investigated. Significant amounts of another isoform (HS3) in addition to HS1 and HS2 were also detectable in a subgroup of these muscles. All the human fetal skeletal muscles at 20 weeks of gestation expressed HS1 and HS2 isoforms but not HS3. The fetal skeletal muscles, also expressed cardiac troponin T in addition. Unlike the human skeletal muscles, only a single isoform of slow troponin T was detected by antibody CDC4 in both the adult and neonatal rat skeletal muscles. The investigation of fetal rat skeletal muscles using the same antibody, however, detected the presence of not only the embryonic cardiac and adult slow skeletal isoforms but also five additional not previously described isoforms (Es1-Es5) of troponin T. These embryonic isoforms, Es1-Es5, were undetectable in the postnatal skeletal muscles although their small amounts could be detected in the neonatal rat hearts. The analysis of individual skeletal muscles from different parts of the body at different stages of fetal development showed marked variations in both the composition of troponin T isoforms and the time sequence of their transitions in each muscle.
Helicobacter species are newly emerging bacteria with great public implications but till now its epidemiology is not fully understood; so, this study was conducted to investigate the possible role of ruminants in the epidemiology of these pathogens. For this purpose, fecal samples were collected from 149 animals (76 sheep, 33 goats, 21 cattle, and 19 buffaloes) and stool specimens from 10 animal caretakers in intimate contact with the examined animals. All samples were examined for the presence of Helicobacter species through detection of Helicobacter genus specific 16S rRNA using PCR. Then, all positive Helicobacter spp. amplicons were sequenced to recognize their species through BLAST analysis at GenBank. The overall prevalence of Helicobacter spp. was 14.8% while the distribution among the different animals was 26.3%, 3%, 4.8%, and 0% in sheep, goats, cattle, and buffaloes respectively. Helicobacter canis was the predominant species and detected only in sheep (21%) and goats (3%). Moreover, Helicobacter winghamensis and Helicobacter canadensis were also detected in sheep but not in other animals, whereas the only positive bovine sample was identified as Helicobacter bovis. On the other hand, 4 out of 10 humans were positive for Helicobacter spp. and all sequences were identified as H. canis. The sequences identity matrix and phylogenetic analysis of H. canis sequences from humans and sheep contacts revealed that one human sequence was identical to that of sheep and making sister group clade, which prove the zoonotic transmission of this pathogen between sheep and human contacts. However, our findings highlight sheep as a potential reservoir for H. canis, further researches are needed to address the potential role of sheep in the food-borne transmission of such emerging pathogen.
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