The correlation between RT-PCR and ELISA assay on hepatitis C positive serum samples

Bahadar, Noor; Ali, Fawad; Israr, Muhammad; Ahmad, Shabeer; Pr, Jilin

doi:10.19045/bspab.2016.50087

Cited by 4 publications

(5 citation statements)

References 25 publications

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“…The sensitivity and specificity of both the strips are above 99% and 98%, respectively. All the positive samples on ICT were further confirmed by 3rd Generation Enzyme- Linked Immunosorbent Assay (ELISA) (EASE BN-96 TMB, Taiwan) as previously described [ 10 , 11 ].…”

Section: Methodsmentioning

confidence: 99%

“…The sample with absorbance value greater than or equal to the cut-off value i.e. (2.00) was considered reactive for HBsAg and HCV-Ab while the sample with absorbance value less than the cut-off value was considered HBsAg and HCV-Ab negative [ 11 , 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…ICT and ELISA) have a relative sensitivity and specificity of above 99% when compared to the confirmatory test and so can give accurate results. Moreover, the same screening methods were adopted previously [10][11][12].…”

Section: Study Limitationsmentioning

confidence: 99%

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Seroepidemiology and associated risk factors of hepatitis B and C virus infections among pregnant women attending maternity wards at two hospitals in Swabi, Khyber Pakhtunkhwa, Pakistan

et al. 2021

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Background & aim Hepatitis B and C infections are global issues that are associated with a massive financial burden in developing countries where vertical transmission is the major mode and remains high. This cross-sectional study was designed to investigate the seroepidemiology and associated risk factors of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections among 375 pregnant women attending antenatal care health facilities at Bacha Khan Medical Complex (BKMC) Shahmansoor and District Head Quarter (DHQ) Hospital Swabi, Khyber Pakhtunkhwa, Pakistan. Methodology From a total of 375 pregnant women selected using systematic random sampling from both hospitals, 10 ml of blood samples were collected and alienated serum was examined for indicators identification through the Immuno-Chromatographic Test (ICT) and 3rd Generation Enzyme-Linked Immunosorbent Assay (ELISA). A pre-structured questionnaire was used to collect the socio-demographic data and possible risk factors. The data was analyzed via SPSS 23.0 statistical software. A chi-square analysis was performed to determine the association between variables. P-value < 0.05 was set statistically significant. Results The overall frequency of HBV and HCV among 375 pregnant women involved in the study was 3.7% and 2.1% respectively. None of the pregnant women were co-infected with HBV and HCV. Dental extraction (P = 0.001) and blood transfusion (P = 0.0005) were significantly allied with HBV infection while surgical procedure (P = 0.0001) was significantly associated with HCV infection. Moreover the sociodemographic characteristics: residential status (P = 0.017) and educational level (P = 0.048) were found significant risk factors of HBsAg and maternal age (P = 0.033) of anti-HCV, respectively. Conclusion & recommendation HBV and HCV infections are intermediary endemic in the study area. A higher prevalence of HBV was detected among pregnant mothers with a history of dental extraction, history of blood transfusion, resident to the urban area and low educational level. The age and surgical procedures were the potential risk factors found significantly associated with HCV positivity among pregnant mothers in our setup. Future negotiations to control vertical transmission should include routine antenatal screening for these infections early in pregnancy and the requirement of efficient preventive tools including the birth dose of the hepatitis B vaccine in combination with hepatitis B immune globulins to the neonate.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Seroepidemiology and associated risk factors of hepatitis B and C virus infections among pregnant women attending maternity wards at two hospitals in Swabi, Khyber Pakhtunkhwa, Pakistan

et al. 2021

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“…A comparative study in Peshawar city was done on three hundred samples for detection of HCV infection using ELISA, of which 14 samples were positive. These 14 samples were subsequently re-analyzed using the RT-PCR method that detected the HCV infection in only 5 samples 19 . Similarly, Kwenti et al (2011) reported that the maximum sensitivity and specificity of the RT-PCR 20 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Thyroid Hormones in Chronic Hepatitis C Patients on Oral Antiviral Therapy

Mahmood¹,

Sardar²,

Mohammad³

et al. 2022

PJMHS

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Background: Hepatitis C virus (HCV) is a major cause of morbidity and mortality. About 71 million people around the world are chronically infected with HCV, however, in Pakistan, approximately 12 million people are suffering from this dreadful virus. In Pakistan, approximately 6.2 % of population is infected with HCV and its treatment rate is approximately 1 %. The aim of this research was to assess thyroid hormones level in chronic HCV patients which were taking the oral-antiviral therapy. Methods: A total of 1650 suspects were screened by immunochromatographic technique. Then ELISA technique were used to confirmed the sample and PCR were also performed. All data were analyzed through Microsoft Excel and SPSS-21. Results: Out of total ICT positive, 117 patients were positive for HCV. Subsequently, all positive samples were exposed to Enzyme linked Immunosorbant assay, out of 117 tested samples 112 (6.7 %) were found positive. However, the final confirmation was done by using Real Time PCR, which showed 109 (6.6 %) patients positive for HCV. Among these 109 HCV patients, the age-wise prevalence of HCV patients was observed maximum 35 (32 %) in age group (55-74 years). Furthermore, these 109 patients were tested for the thyroid dysfunction, before and after anti-viral therapy (Sofosbuvir and Daclatasvir therapy) which was recommended by World Health Organization. Out of 109 HCV positive patients the frequency, 92 (84.6 %) showed euthyroidism, 6 (5.5 %) hypothyroidism and 11 (10 %) hyperthyroidism. Thyroid dysfunction was high (n=17, 15.6 %) in patients with age above 50 years. Six (35.2 %) of thyroid dysfunction patients revealed hypothyroidism and hyperthyroidism was observed in 11 (64.7 %) patients. Mean values of thyroid function test before antiviral therapy were TSH = 3.88 ± 1.0, TT3 = 1.15 ± 0.58, and TT4 = 7.7 ± 01.99 and mean values after three months therapy were TSH = 2.49±1.72, TT3 =1.38 ± 0.69 and TT4 = 8.78 ± 2.29. Conclusion: It is concluded that HCV infected individuals on oral antiviral drugs have an effect on the thyroid gland, therefore, HCV patients should be frequently screened for thyroid disorders before and after treatment to ensure euthyroid status. Keywords: Thyroid hormones, Hepatitis, Oral Anti-viral therapy, Thyroidism

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“…In Pakistan, HCV was found as the main factor responsible for the development of liver cancer (Khan et al, 2009), and about 70% patients of HCC were found infected with HCV whereas HBV co infection has been found in 20% of HCC positive cases (Butt et al, 2013). In Pakistan, the prevalence of hepatic inflammation is alarming showing 4.8% positivity for anti HCV antibodies and for 2.5% HBV surface antigen with (Abbas, Jafri, & Hamid, 2010;Bahadar, Khan, Israr, & Ahmad, 2016). The prolonged untreated infection leads to the development of chronic liver disease that gradually progresses to fibrotic stage, cirrhosis, hepatic damage and finally HCC (Hajarizadeh, Grebely, & Dore, 2013).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Serum Markers for Early Diagnosis of HCV Induced Hepatocellular Carcinoma

SIDDIQUI

Tunio

Bano

et al. 2022

USJAS

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Hepatocellular carcinoma is a global public health problem which is intensified at developing countries due to lack of early diagnosis. Since Hepatitis C virus related morbidity accounts five million people in Pakistan, rate of morbidity and mortality of HCV related Hepatocellular carcinoma infections is high in Pakistan, and it is attributed to its diagnosis after its development. The public has the lack of awareness about the importance and benefits of liver biopsy testing; therefore, improved, and non-invasive methods of HCC diagnosis are urgently needed. The aim of the present study was to evaluate the importance of serum markers in early diagnosis of HCV induced Hepatocellular carcinoma and to develop a robust set of serological markers for their early detection and diagnosis. A total of 60 HCV positive patients including Chronic Liver disease (n=20), Cirrhosis (n=20), HCC patients (n=20) and 20 healthy volunteers (n=20) were enrolled in this study after an extensive screening process. Blood samples were collected from all the subjects and processed for serum separation followed by immunological, molecular, and biochemical analysis using Enzyme Linked Immunosorbant Assay, Real-Time PCR, complete blood picture, and a range of biochemical tests. Results showed gender wise variability among the subjects from each of the study groups where in the male subjects remained dominant with male and female ratio as 61.75:38.75. The average age of male subjects was observed between 42y, 43y, 49y and 37 years in comparison of female subjects who were 46y, 53y, 50y and 47y in CLD, cirrhosis, Hepatocellular carcinoma, and control groups, respectively. The data has also demonstrated that Hepatocellular carcinoma patients having Alpha Fetoprotein level >1000 showed the increased level of ALP and GGT with decreased HB and HCT. However, the Hepatocellular carcinoma patients having AFP level in the range of 100-500 had total bilirubin level > 3.0, while those having AFP level <100 has all serological markers in normal range. In addition, the patients of HCC showed increased PT with decreased albumin level. The increases level of albumin level (>3-<4.0) was observed from the serum samples of cirrhosis and Chronic liver disease patients. The increase in albumin up to 4.0 accompanied with the increased level of ALP and PT while decreased HB. In conclusion, different biochemical parameters may be used to distinguish the patients having Hepatocellular carcinoma from those suffering from cirrhosis and chronic liver disease induced by HCV infection. The findings of present study may help to enhance the outcome for patients with HCC by enabling the diagnosis to be made at an earlier stage of the disease.

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The correlation between RT-PCR and ELISA assay on hepatitis C positive serum samples

Cited by 4 publications

References 25 publications

Seroepidemiology and associated risk factors of hepatitis B and C virus infections among pregnant women attending maternity wards at two hospitals in Swabi, Khyber Pakhtunkhwa, Pakistan

Seroepidemiology and associated risk factors of hepatitis B and C virus infections among pregnant women attending maternity wards at two hospitals in Swabi, Khyber Pakhtunkhwa, Pakistan

Evaluation of Thyroid Hormones in Chronic Hepatitis C Patients on Oral Antiviral Therapy

Evaluating the Serum Markers for Early Diagnosis of HCV Induced Hepatocellular Carcinoma

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