The contribution of nitrogen fixation to sugarcane (Saccharum officinarum L.), and the identification and characterization of part of the associated diazotrophic bacterial community

Taulé, Cecilia; Mareque, Cintia; Barlocco, Claudia; Hackembruch, Fernando; Reis, Verônica Massena; Sicardi, Margarita; Battistoni, Federico

doi:10.1007/s11104-011-1023-4

Cited by 142 publications

(96 citation statements)

References 75 publications

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“…Based on this data we can assume that a site specificity of bacterial species distribution may be influencing sugarcane bacterial diversity independent of the plant genotype. In addition, representatives of other genera, such as Kosakonia, Klebsiella, Stenotrophomonas, and some Gram-positive or Gram-variable isolates were also found as previously reported by others, using culture dependent or independent methods (Magnani et al, 2010;Taulé et al, 2012;Fischer et al, 2012;Magnani et al, 2013). The occurrence of representatives of Gluconacetobacter as the most abundant and present in both roots and shoots of sugarcane varieties cropped in Alagoas could be explained by the use of LGI-P medium with addition of sugarcane juice in which the initial pH is 5.7 during isolation procedure.…”

Section: Resultssupporting

confidence: 79%

“…Recent studies using methods independent of cultivation also demonstrated a great diversity of sugarcane natural population of diazotrophs, indication that the contribution of biological nitrogen fixation (BNF) plays an important role in this plant. It was found that Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, H. rubrisubalbicans, Burkholderia tropica, B. silvatlantica, Stenotrophomonas, Pantoea and many others are part of the diazotrophic bacteria diversity found distributed in the roots, stems and leaves of sugarcane (Fischer et al, 2012;Taulé et al, 2012;Magnani et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

Antonio

Rouws

Teixeira

et al. 2016

Agraria

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The objective of this work was to study the diversity of diazotrophic bacteria associated to 10 varieties of sugarcane cultivated in two states of the Northeast of Brazil and to identify the isolates by morphological and molecular methods. Diversity was studied by the application of traditional growth-depending microbiological methods: Gram-staining, comparison of colony morphology and growth on several culture media. Phylogenetic taxonomy was performed using 16S rRNA gene sequencing data. Almost all isolates obtained in semi-solid nitrogen free media were classified as Gram-negative and seven colony morphological groups were distinguished after growth on Potato medium. Among the 10 varieties evaluated, RB72454 resulted in higher number of isolates and diversity. The great majority of isolates from most of the genotypes tested showed similar characteristic to the genus Gluconacetobacter based on its peculiar colony morphology and growth characteristics in semi-solid media. Comparison of the 16S rRNA sequence of each isolate to the Genbank showed that they are closely related to Gluconacetobacter, Burkholderia, Kosakonia, Klebsiella and Stenotrophomonas.Key words: biological nitrogen fixation, ecology of diazotrophic bacteria, sugarcane Bactérias diazotróficas associadas a variedades de cana-de-açúcar cultivadas na região Nordeste do Brasil RESUMOO objetivo deste trabalho foi estudar a diversidade de bactérias diazotróficas associadas a 10 variedades de cana cultivadas em dois estados do Nordeste do Brasil e identificar os isolados por métodos morfológicos e moleculares. A diversidade foi estudada através da aplicação de métodos microbiológicos tradicionais dependentes de crescimento: coloração de Gram, comparação da morfologia de colónias e crescimento em vários meios de cultura. Taxonomia filogenética foi realizada utilizando dados de sequenciamento de gene 16S rRNA. Quase todos os isolados obtidos em meios semissólidos livres de nitrogênio foram classificados como Gram-negativos e sete grupos baseados na morfologia de colônias foram distinguidos após crescimento em meio batata. Entre as 10 variedades avaliadas, RB72454 resultou em maior diversidade e número de isolados. A grande maioria dos isolados dos genótipos testados apresentaram característica similar ao gênero Gluconacetobacter com base em seu peculiar crescimento em meios semissólidos e morfologia da colônia. A comparação da sequência do 16S rRNA de cada isolado com sequencias do GenBank mostrou que eles estão intimamente relacionados com Gluconacetobacter,

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Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

Antonio

Rouws

Teixeira

et al. 2016

Agraria

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“…Loiret et al (2004) and Taulé et al (2012) reported the prevalence of isolates from this family, commonly described in association with roots and the rhizosphere, showing potential as phosphate solubilizers and producers of a large amount of indoles.…”

Section: Resultsmentioning

confidence: 99%

“…Maize was selected as a model organism because it also belongs to the Poaceae family, grows fast (Wang et al 2016), and because the same bacterial species have been described in both plants (Taulé et al 2012, Silva et al 2016). …”

Section: Methodsmentioning

confidence: 99%

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Isolation and selection of plant growth-promoting bacteria associated with sugarcane

Rodrigues

Forzani

Soares

et al. 2016

Pesqui. Agropecu. Trop.

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Microorganisms play a vital role in maintaining soil fertility and plant health. They can act as biofertilizers and increase the resistance to biotic and abiotic stress. This study aimed at isolating and characterizing plant growth-promoting bacteria associated with sugarcane, as well as assessing their ability to promote plant growth. Endophytic bacteria from leaf, stem, root and rhizosphere were isolated from the RB 867515 commercial sugarcane variety and screened for indole acetic acid (IAA) production, ability to solubilize phosphate, fix nitrogen and produce hydrogen cyanide (HCN), ammonia and the enzymes pectinase, cellulase and chitinase. A total of 136 bacteria were isolated, with 83 of them presenting some plant growth mechanism: 47 % phosphate solubilizers, 26 % nitrogen fixers and 57 % producing IAA, 0.7 % HCN and chitinase, 45 % ammonia, 30 % cellulose and 8 % pectinase. The seven best isolates were tested for their ability to promote plant growth in maize. The isolates tested for plant growth promotion belong to the Enterobacteriaceae family and the Klebsiella, Enterobacter and Pantoea genera. Five isolates promoted plant growth in greenhouse experiments, showing potential as biofertilizers.

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Plant Probiotics for Nutrient Acquisition by Agriculturally Important Grasses: A Comprehensive Review of the Science and the Application

Swift

Denton

Melino

2019

Annual Plant Reviews Online

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Intensification of agricultural land and the overuse of inorganic fertilisers has led to soil acidification, depletion of organic matter, and environmental pollution. Approaches to protect soil health, including the enhancement of microbial diversity, are integral to improving crop productivity and food security. Metagenomics has rapidly improved our understanding of soil microbial diversity and function, while genetic techniques have helped to dissect the complex signal exchange between microorganisms and plants. This article presents and evaluates reported beneficial effects of plant growth‐promoting bacteria (PGPB), focussing on those capable of mobilising or solubilising nutrients and/or stimulating plant growth when applied to agriculturally important grasses. The agricultural industry has capitalised on these scientific advancements, generating microbial formulations for specific crop responses. However, scientific methodologies must be applied in order to overcome inherent limitations of many PGPBs including their inability to be cultured, their poorly defined or multiple modes of action, a low level of integration with the crop partner, and an unpredictability in translating beneficial plant responses to the field. Novel approaches such as engineered rhizospheres, enhancing endophytic systems, cereal nodule development, and the use of inoculant consortiums will be necessary to sustain growth in the biofertiliser industry.

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The contribution of nitrogen fixation to sugarcane (Saccharum officinarum L.), and the identification and characterization of part of the associated diazotrophic bacterial community

Cited by 142 publications

References 75 publications

Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

Diazotrophic bacteria associated to sugarcane varieties cropped at Northeast Region of Brazil

Isolation and selection of plant growth-promoting bacteria associated with sugarcane

Plant Probiotics for Nutrient Acquisition by Agriculturally Important Grasses: A Comprehensive Review of the Science and the Application

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