Isolation and selection of plant growth-promoting bacteria associated with sugarcane

Abstract

Microorganisms play a vital role in maintaining soil fertility and plant health. They can act as biofertilizers and increase the resistance to biotic and abiotic stress. This study aimed at isolating and characterizing plant growth-promoting bacteria associated with sugarcane, as well as assessing their ability to promote plant growth. Endophytic bacteria from leaf, stem, root and rhizosphere were isolated from the RB 867515 commercial sugarcane variety and screened for indole acetic acid (IAA) production, ability to solubilize phosphate, fix nitrogen and produce hydrogen cyanide (HCN), ammonia and the enzymes pectinase, cellulase and chitinase. A total of 136 bacteria were isolated, with 83 of them presenting some plant growth mechanism: 47 % phosphate solubilizers, 26 % nitrogen fixers and 57 % producing IAA, 0.7 % HCN and chitinase, 45 % ammonia, 30 % cellulose and 8 % pectinase. The seven best isolates were tested for their ability to promote plant growth in maize. The isolates tested for plant growth promotion belong to the Enterobacteriaceae family and the Klebsiella, Enterobacter and Pantoea genera. Five isolates promoted plant growth in greenhouse experiments, showing potential as biofertilizers.

Isolation and prospection of diazotrophic rhizobacteria associated with sugarcane under organic management

“…Enterobacter sp. NC25 and NU33 strains produced 80.42 and 77.41 mg mL -1 , respectively, similar to the strains reported by Rodrigues et al (2016) when evaluating Enterobacter sp. rhizospheres from traditionally managed sugarcane.…”

Isolation and characterization of plant growth promoting endophytic bacteria isolated from Vigna radiata

“…HCN Production: Hydrogen cyanide is a volatile compound which exhibit antifungal properties protecting plants against various soil borne pathogens and help in plant growth indirectly (Ramette et al 2003).There are several reports on HCN production by endophytes (Ngoma et al 2013;Etesami et al 2014;Rodrigues et al 2016). All the endophytic isolates were producing HCN and MJiN9, MJiR4, MJiR9, MJiR13 were the best among these (Fig.…”

TRIAGEM DE POTENCIALIDADES BIOTECNÓLOGICAS DE BACTÉRIAS ENDOFÍTICAS ISOLADAS DE FOLHAS Gossypium hirsutum L.

“…Embora os endofíticos encontrados nesse estudo apresentem potencial na solubilização de fosfato, Rodrigues et al (2016) destacam que para que a solubilização aconteça, os micro-organismos devem estar próximos a fonte de fósforo, haja vista que a solubilização de fosfato envolve muitas vezes a liberação de ácidos sob a fonte de fosfato. Dessa forma, é necessário avaliar posteriormente a capacidade desses endofíticos em habitar a região de solo aderida as raízes (rizosfera).…”