Microorganisms play a vital role in maintaining soil fertility and plant health. They can act as biofertilizers and increase the resistance to biotic and abiotic stress. This study aimed at isolating and characterizing plant growth-promoting bacteria associated with sugarcane, as well as assessing their ability to promote plant growth. Endophytic bacteria from leaf, stem, root and rhizosphere were isolated from the RB 867515 commercial sugarcane variety and screened for indole acetic acid (IAA) production, ability to solubilize phosphate, fix nitrogen and produce hydrogen cyanide (HCN), ammonia and the enzymes pectinase, cellulase and chitinase. A total of 136 bacteria were isolated, with 83 of them presenting some plant growth mechanism: 47 % phosphate solubilizers, 26 % nitrogen fixers and 57 % producing IAA, 0.7 % HCN and chitinase, 45 % ammonia, 30 % cellulose and 8 % pectinase. The seven best isolates were tested for their ability to promote plant growth in maize. The isolates tested for plant growth promotion belong to the Enterobacteriaceae family and the Klebsiella, Enterobacter and Pantoea genera. Five isolates promoted plant growth in greenhouse experiments, showing potential as biofertilizers.

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RESUMOAs enteroparasitoses representam um grave problema de saúde pública, sendo as crianças em idade escolar as mais acometidas. O estudo teve como objetivo estabelecer a ocorrência de parasitoses intestinais em crianças atendidas pelo PETI (Programa de erradicação do trabalho infantil) nos distritos de Interlândia e Sousânia no ano de 2013. Foram avaliadas 48 crianças de 05 a 14 anos de idade atendidas pelo PETI. De cada criança foram coletadas três amostras de fezes em dias alternados e a análise parasitológica foi feita pelo método de Hoffman. Da população estudada 37,5% apresentava algum tipo de parasitose. Das amostras analisadas foram encontradas somente protozoários, sendo identificados Entamoeba coli 44%, Endolimax nana 22,5%, Giardia lamblia 22,5%, Entamoeba histolytica 11%. Sugerese que os resultados obtidos sejam resposta da fragilidade das circunstâncias de saneamento básico, e situações de moradia e costumes de asseio das crianças estudadas nos distritos de Intêrlandia e Sousânia. PALAVRAS-CHAVE: crianças, enteroparasitoses, protozoários. OCCURRENCE OF ENTEROPARASITOSIS IN CHILDREN ATTENDEDING CHILD LABOR ERRADICACION PROGRAM IN DISTRICTS OF INTERLÂNDIA AND SOUSÂNIA IN ANÁPOLIS-GO IN THE YEAR OF 2013ABSTRACT The enteroparasitosis represent a serious public health issue, and the school age children are the most affected. The study aimed to establish the occurrence of intestinal parasites in children assisted by PETI (child labor eradication program) in the districts of Interlândia and Sousânia in 2013. Forty eight children of 05 to 14 years old assisted by PETI were evalueted. From each child was collected three stool samples on alternate days and parasitological analysis was done by Hoffman method. Of the population analyzed, 37.5% had some type of parasitosis . In the samples were found only protozoan,such as Entamoeba coli 44%, Endolimax nana

Phenolic compounds are common pollutants in industrial effluents and pose a significant risk to the environment. The microbial capacity to degrade these molecules makes the prospection of bacteria with this ability very strategic for bioremediation processes in contaminated areas. This study was carried out to isolate bacteria with phenol degrading capacity from Brazilian mangrove soil. Microorganisms were evaluated for tolerance and the ability to use this substance as a single source of carbon. Two bacterial isolates were obtained: MF-1 and MF-2. The microorganisms were identified, respectively, as Bacillus cereus and Bacillus circulans by sequencing the 16S rRNA gene. The isolates exhibited tolerance to phenol at concentrations up to 600 mg.L-1 when grown in Bushnell-Haas Agar (BHA) and 1,500 mg.L-1 in nutrient agar (NA). As for the use of phenol as a single carbon source, the microorganisms were grown in concentrations of 100, 200 and 300 mg.L-1 of the compound. The isolate MF-1 presented a percentage consumption of 22.43, 11.52 and 3.33%, respectively, for each tested concentration. Although there was a decrease in phenol consumption in response to the increased concentration of this substance, bacterial growth was not affected. The best results were obtained for the isolate MF-2, in which consumption of phenol was, respectively, 21.54, 20.54 and 28.85% for these concentrations. Likewise, the increase in phenol concentration caused no reduction in the growth of this isolate.

RESUMO A biorremediação utiliza substâncias emulsificadoras, surfactantes, para mitigar contaminações ambientais. Nesse estudo, foi realizado o isolamento de 27 microorganismos a partir de amostras de solo próximas a postos de combustíveis e desses, foi selecionando através de atividade hemolítica um isolado, Bacillus sp. BRUFG 18, como produtor de biossurfactantes. Para a caracterização da produção de bioemulsificantes, amostras de meio mínimo acrescido de glucose ou óleo diesel foram coletadas por sete dias e o pH, índice de emulsificação, crescimento microbiano e consumo de glucose foram monitorados. No sétimo dia, o pH variou pouco, entre 7,83 a 8,00, e ocorreu um melhor crescimento de Bacillus sp. BRUFG 18 em meio contendo óleo diesel. Os índices de emulsificação variaram entre 72 e 75%. Os resultados evidenciam a potencialidade de se produzir biossurfactantes comercialmente com uma pequena variação de pH e utilizando uma fonte de carbono abundante e de baixo custo, como a glucose, a partir de um isolado bacteriano. PALAVRAS-CHAVE: Bacillus, biorremediação, biossurfactantes, glucose, índice de emulsificação, óleo diesel. PRODUCTION OF BIOSURFACTANTS BY Bacillus sp. IN MINIMAL MEDIUM CONTAINING GLUCOSE ABSTRACT Bioremediation uses emulsifying substances, surfactants, to mitigate environmental contaminations. On this study, it was carried out the isolation of 27 microorganisms from soil samples near gas stations and from these, it was selected through hemolytic activity one strain, Bacillus sp. BRUFG 18, as a biosurfactant producer. To the characterization of the production of bioemulsifiers, samples containing minimum media supplied with glucose or diesel fuel, were collected in 7 days and pH, emulsification index, microbial growth and glucose consumption were measured. On