The Construction of Full-Length cDNA Library for Otodectes cynotis

Li, Hu; Zhao, Yongtao; Niu, Dongling; Yang, Rui; Zeng, Ji-Hui

doi:10.2478/s11686-019-00034-y

Cited by 3 publications

(1 citation statement)

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“…Only a few studies of its molecular biology ( Salib and Baraka, 2011 ; Taenzler et al, 2017 ). As a result, there are only a limited number genes from O. cynotis mites deposited in public databases ( Hu et al, 2019 ). In the present study, we aim to use single-molecule real-time sequencing (SMRT-Seq) and Illumina based RNA-sequencing (RNA-Seq) to obtain sequence information of O. cynotis mites, analyze the expression of allergen genes at different life stages, and determine the protein-protein interaction (PPI) network of O. cynotis mites.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Otodectes cynotis in Different Developmental Stages

Zhang

et al. 2022

Front. Microbiol.

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The mite Otodectes cynotis is distributed worldwide and parasitism the ear canals of cats and dogs, causing otitis externa. Molecular biology of O. cynotis is poorly understood, with only a few genes being deposited in public databases. In the present study, we aimed to perform transcriptome analysis of O. cynotis using SMRT and Illumina sequencing of RNA from different development stages. SMRT-Seq of O. cynotis demonstrated 5,431 final transcripts, including 406 long non-coding RNAs and 2,698 differentially expressed genes (DEGs), including 1,357 up-regulated genes and 1,341 down-regulated genes between adult mites and nymph/larva. A total of 397 putative allergen genes were detected, 231 of which were DEGs. Among them, 77 were homologous of known mite allergens. The expression level of allergen genes hints at the pathogenicity of mites in different life stages, and the protein interaction network analysis could identify possible key genes in the pathogenic mechanism. Intriguingly, Gene Ontology analysis showed that most of the (DEGs) were associated with the terms hydrolase activity and proteolysis. Kyoto Encyclopedia of genes and genomes (KEGG) analysis identified drug metabolism-cytochrome P450 signal pathway as one of the top pathways. SMRT-Seq of the full-length transcriptome of O. cynotis was performed first, and a valuable resource was acquired through the combination analysis with the Illumina sequencing data. The results of our analyses provide new information for further research into Otodectes cynotis.

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Section: Introductionmentioning

confidence: 99%