De Novo RNA-seq and Functional Annotation of Haemaphysalis longicornis

Niu, Dongling; Zhao, Yongtao; Yang, Yanan; Yang, Rui; Gong, Xiaochun; Li, Hu

doi:10.2478/s11686-019-00103-2

Cited by 7 publications

(3 citation statements)

References 59 publications

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“…From six libraries, 271,494,907 reads were used for assembly that generated 61,800 contigs that were analyzed, of which ~40,000 found BLAST hits. For engorged female H. longicornis , ~53 million reads generated 65,916 contigs, of which 23,339 could be annotated (Niu et al, 2019 ). Whole body transcriptomes for larvae (64,474,326) and nymphs (81,612,022) were also generated (Guo et al, 2019 ).…”

Section: Next-generation Sequencing Of Whole Body Transcriptomesmentioning

confidence: 99%

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Mans

2020

Front. Cell. Infect. Microbiol.

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Species have definitive genomes. Even so, the transcriptional and translational products of the genome are dynamic and subject to change over time. This is especially true for the proteins secreted by ticks at the tick-host feeding interface that represent a complex system known as the sialoverse. The sialoverse represent all of the proteins derived from tick salivary glands for all tick species that may be involved in tick-host interaction and the modulation of the host's defense mechanisms. The current study contemplates the advances made over time to understand and describe the complexity present in the sialoverse. Technological advances at given periods in time allowed detection of functions, genes, and proteins enabling a deeper insight into the complexity of the sialoverse and a concomitant expansion in complexity with as yet, no end in sight. The importance of systematic classification of the sialoverse is highlighted with the realization that our coverage of transcriptome and proteome space remains incomplete, but that complete descriptions may be possible in the future. Even so, analysis and integration of the sialoverse into a comprehensive understanding of tick-host interactions may require further technological advances given the high level of expected complexity that remains to be uncovered.

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Section: Next-generation Sequencing Of Whole Body Transcriptomesmentioning

confidence: 99%

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Mans

2020

Front. Cell. Infect. Microbiol.

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“…In China, F. tularensis was first reported in 1957 [ 6 ]. In 1959, Heilongjiang Province reported the first human infection with hare fever [ 7 ]. Subsequently, natural foci of tularemia were confirmed in Tibet, Xinjiang, and Gansu [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Detection and Genotyping of Francisella tularensis in Animal Hosts and Vectors from Six Different Natural Landscape Areas, Gansu Province, China

Fang

Wang

et al. 2021

Computational and Mathematical Methods in Medicine

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Objective. Tularemia, also known as hare fever, is caused by the bacterium Francisella tularensis (F. tularensis) transmitted through diseased wild animals, blood sucking insects, or contaminated water or food, which is distributed worldwide. The purpose of this study was to investigate F. tularensis infection in animal hosts and vectors from six different natural landscape areas in Gansu Province and to identify the genotypes of the detected F. tularensis. Methods. Rodents were captured by snap traps, and ticks were collected by dragging a cloth over the vegetation or from domestic animals. After species identification, DNA was isolated from the captured animals and detected by nested PCR assays targeting the F. tularensis fopA gene. The positive samples were further amplified to discriminate the species, and another two short-sequence tandem repeat regions (SSTR) were amplified to identify their genotypes. All positive fragments were sequenced and analyzed by ClustalX (5.0) and DNAClub software. Results. A total of 407 rodents of 12 species were captured, among which six rodent species were positive for F. tularensis, with an overall prevalence of 3.93%. The geographical difference in infection rate was statistically significant. At the SSTR9 locus, there were 7 genotypes among positive rodent samples. A total of 1864 ticks were tested for evidence of tularemia by nested PCR assays, 69 of which were positive, with an average positive rate of 3.70% for F. tularensis in ticks. The positive rates were significantly different among different regions. Seven genotypes were identified at the SSTR9 locus, one of which seemed dominant in positive tick samples. All positive samples had the same genotype at the SSTR16 locus. Conclusion. There is natural infection of F. tularensis among animal vectors and hosts in Gansu Province, with diverse genotypes.

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“…Haemaphysalis longicornis ticks progress through four stages of life, the egg, larva, nymph, and adult stages, through their complete life cycle. Muscle performs necessary functions during blood sucking by moving the coxae of the appendages, retracting the chelicerae, and controlling pharyngeal action [11]. In addition, Drosophila Mlp84B can cooperate with D-titin to maintain muscle structural integrity [12].…”

Section: Introductionmentioning

confidence: 99%

Characterization of an MLP Homologue from Haemaphysalis longicornis (Acari: Ixodidae) Ticks

et al. 2020

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Members of the cysteine-rich protein (CRP) family are known to participate in muscle development in vertebrates. Muscle LIM protein (MLP) belongs to the CRP family and has an important function in the differentiation and proliferation of muscle cells. In this study, the full-length cDNA encoding MLP from Haemaphysalis longicornis (H. longicornis; HLMLP) ticks was obtained by 5′ rapid amplification of cDNA ends (RACE). To verify the transcriptional status of MLP in ticks, HLMLP gene expression was assessed during various developmental stages by real-time PCR (RT-PCR). Interestingly, HLMLP expression in the integument was significantly (P < 0.01) higher than that observed in other tested tissues of engorged adult ticks. In addition, HLMLP mRNA levels were significantly downregulated in response to thermal stress at 4 °C for 48 h. Furthermore, recombinant HLMLP was expressed in Escherichia coli, and Western blot analysis showed that rabbit antiserum against H. longicornis adults recognized HLMLP and MLPs from different ticks. Ten 3-month-old rabbits that had never been exposed to ticks were used for the immunization and challenge experiments. The rabbits were divided into two groups of five rabbits each, where rabbits in the first group were immunized with HLMLP, while those in the second group were immunized with phosphate-buffered saline (PBS) diluent as controls. The vaccination of rabbits with the recombinant HLMLP conferred partial protective immunity against ticks, resulting in 20.00% mortality and a 17.44% reduction in the engorgement weight of adult ticks. These results suggest that HLMLP is not ideal as a candidate for use in anti-tick vaccines. However, the results of this study generated novel information on the MLP gene in H. longicornis and provide a basis for further investigation of the function of this gene that could potentially lead to a better understanding of the mechanism of myofiber determination and transformation

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De Novo RNA-seq and Functional Annotation of Haemaphysalis longicornis

Cited by 7 publications

References 59 publications

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Detection and Genotyping of Francisella tularensis in Animal Hosts and Vectors from Six Different Natural Landscape Areas, Gansu Province, China

Characterization of an MLP Homologue from Haemaphysalis longicornis (Acari: Ixodidae) Ticks

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