Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Mans, Ben J.

doi:10.3389/fcimb.2020.574405

Cited by 21 publications

(30 citation statements)

References 174 publications

(249 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of previous studies in several ixodid species have reported that feeding progression is linked with a temporal transcriptional regulation of salivary gene expression [ 50 , 58 – 60 ]. For argasids, the information on their sialomes is scarcer, and information related to the dynamics of salivary gene expression and protein synthesis is almost inexistent [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…In argasids, these studies have been scarcer and, as far as we know, the sialomes of only six species have been published, i.e. Ornithodoros coriaceus , Ornithodoros parkeri , Ornithodoros turicata , Ornithodoros rostratus , Argas monolakensis and Antricola delacruzi [ 19 ]. These sialomes have uncovered thousands of protein-coding sequences and large multigene protein families, many of them conserved between both tick families, which underlines that tick sialomes and saliva composition are highly complex and functionally redundant [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Ornithodoros coriaceus , Ornithodoros parkeri , Ornithodoros turicata , Ornithodoros rostratus , Argas monolakensis and Antricola delacruzi [ 19 ]. These sialomes have uncovered thousands of protein-coding sequences and large multigene protein families, many of them conserved between both tick families, which underlines that tick sialomes and saliva composition are highly complex and functionally redundant [ 19 ]. All these tick salivary protein sequences have been compiled and classified into a recently constructed database (TickSialoFam) [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sialotranscriptomics of the argasid tick Ornithodoros moubata along the trophogonic cycle

et al. 2021

View full text Add to dashboard Cite

The argasid tick Ornithodoros moubata is the main vector of human relapsing fever (HRF) and African swine fever (ASF) in Africa. Salivary proteins are part of the host-tick interface and play vital roles in the tick feeding process and the host infection by tick-borne pathogens; they represent interesting targets for immune interventions aimed at tick control. The present work describes the transcriptome profile of salivary glands of O. moubata and assesses the gene expression dynamics along the trophogonic cycle using Illumina sequencing. De novo transcriptome assembling resulted in 71,194 transcript clusters and 41,011 annotated transcripts, which represent 57.6% of the annotation success. Most salivary gene expression takes place during the first 7 days after feeding (6,287 upregulated transcripts), while a minority of genes (203 upregulated transcripts) are differentially expressed between 7 and 14 days after feeding. The functional protein groups more abundantly overrepresented after blood feeding were lipocalins, proteases (especially metalloproteases), protease inhibitors including the Kunitz/BPTI-family, proteins with phospholipase A2 activity, acid tail proteins, basic tail proteins, vitellogenins, the 7DB family and proteins involved in tick immunity and defence. The complexity and functional redundancy observed in the sialotranscriptome of O. moubata are comparable to those of the sialomes of other argasid and ixodid ticks. This transcriptome provides a valuable reference database for ongoing proteomics studies of the salivary glands and saliva of O. moubata aimed at confirming and expanding previous data on the O. moubata sialoproteome.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sialotranscriptomics of the argasid tick Ornithodoros moubata along the trophogonic cycle

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Additionally, the sialotranscriptome of the argasid tick Ornithodoros moubata has recently been obtained in a study analogous to the present one [ 43 ]. These tick sialomes have revealed thousands of protein-coding sequences and large multigene protein families, many of them conserved between both tick families, reflecting high complexity and functional redundancy in the composition of tick sialomes and saliva, especially among ixodids [ 28 , 44 ]. A recently constructed database (TickSialoFam) has compiled and classified all these tick salivary protein sequences [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle

et al. 2021

View full text Add to dashboard Cite

Background The argasid tick Ornithodoros erraticus is the main vector of tick-borne human relapsing fever (TBRF) and African swine fever (ASF) in the Mediterranean Basin. Tick salivary proteins secreted to the host at the feeding interface play critical roles for tick feeding and may contribute to host infection by tick-borne pathogens; accordingly, these proteins represent interesting antigen targets for the development of vaccines aimed at the control and prevention of tick infestations and tick-borne diseases. Methods To identify these proteins, the transcriptome of the salivary glands of O. erraticus was de novo assembled and the salivary gene expression dynamics assessed throughout the trophogonic cycle using Illumina sequencing. The genes differentially upregulated after feeding were selected and discussed as potential antigen candidates for tick vaccines. Results Transcriptome assembly resulted in 22,007 transcripts and 18,961 annotated transcripts, which represent 86.15% of annotation success. Most salivary gene expression took place during the first 7 days after feeding (2088 upregulated transcripts), while only a few genes (122 upregulated transcripts) were differentially expressed from day 7 post-feeding onwards. The protein families more abundantly overrepresented after feeding were lipocalins, acid and basic tail proteins, proteases (particularly metalloproteases), protease inhibitors, secreted phospholipases A2, 5′-nucleotidases/apyrases and heme-binding vitellogenin-like proteins. All of them are functionally related to blood ingestion and regulation of host defensive responses, so they can be interesting candidate protective antigens for vaccines. Conclusions The O. erraticus sialotranscriptome contains thousands of protein coding sequences—many of them belonging to large conserved multigene protein families—and shows a complexity and functional redundancy similar to those observed in the sialomes of other argasid and ixodid tick species. This high functional redundancy emphasises the need for developing multiantigenic tick vaccines to reach full protection. This research provides a set of promising candidate antigens for the development of vaccines for the control of O. erraticus infestations and prevention of tick-borne diseases of public and veterinary health relevance, such as TBRF and ASF. Additionally, this transcriptome constitutes a valuable reference database for proteomics studies of the saliva and salivary glands of O. erraticus.

show abstract

“…Transcriptomics enabled by RNA next generation sequencing technologies (RNA-Seq) has revealed the complex dynamics of the salivary glands of ticks as evident in the growing list of published tick salivary gland transcriptomes generated from various tick life stages and/or genders 4 . These studies identified thousands of transcripts coding for numerous protein families secreted over the course of feeding with defined differential expression patterns between male and female ticks.…”

Section: Introductionmentioning

confidence: 99%

De novo assembled salivary gland transcriptome and expression pattern analyses for Rhipicephalus evertsi evertsi Neuman, 1897 male and female ticks

Pienaar

Klerk

Castro

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

Ticks secrete proteins in their saliva that change over the course of feeding to modulate the host inflammation, immune responses, haemostasis or may cause paralysis. RNA next generation sequencing technologies can reveal the complex dynamics of tick salivary glands as generated from various tick life stages and/or males and females. The current study represents 15,115 Illumina sequenced contigs of the salivary gland transcriptome from male and female Rhipicephalus evertsi evertsi ticks of early, mid and late feeding stages from 1320 separate assemblies using three short read assemblers. The housekeeping functional class contributed to the majority of the composition of the transcriptome (80%) but with lower expression (51%), while the secretory protein functional class represented only 14% of the transcriptome but 46% of the total coverage. Six percent had an unknown status contributing 3% of the overall expression in the salivary glands. Platelet aggregation inhibitors, blood clotting inhibitors and immune-modulators orthologous to the ancestral tick lineages were confirmed in the transcriptome and their differential expression during feeding in both genders observed. This transcriptome contributes data of importance to salivary gland biology and blood feeding physiology of non-model organisms.

show abstract

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Cited by 21 publications

References 174 publications

Sialotranscriptomics of the argasid tick Ornithodoros moubata along the trophogonic cycle

Sialotranscriptomics of the argasid tick Ornithodoros moubata along the trophogonic cycle

RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle

De novo assembled salivary gland transcriptome and expression pattern analyses for Rhipicephalus evertsi evertsi Neuman, 1897 male and female ticks

Contact Info

Product

Resources

About