The Complete Nucleotide Sequence of Swine Vesicular Disease Virus

Abstract

SUMMARYThe complete nucleotide sequence of the genome of the enterovirus swine vesicular disease virus (SVDV ; H/3 '76) isolated from a healthy pig has been determined using molecular cloning and DNA sequencing techniques. The RNA genome was 7400 nucleotides long, excluding the poly(A) tract, and appeared to encode a single polyprotein of 2185 amino acids. The predicted amino acid sequence of the polyprotein showed close homology (around 90 ~) to that of the previously sequenced coxsackieviruses B1, B3 and B4, and also showed homology (around 60~) to that of poliovirus. This homology allows us to predict the possible cleavage sites of the polyprotein and to identify other features of structural and functional significance, which seem to be important to the biological integrity of the virus. A detailed analysis of homology between SVDV and coxsackieviruses shows that non-structural proteins are highly conserved whereas the structural proteins are less well conserved. The 5' and 3' noncoding regions are also conserved, although there are several divergent nuclcotide stretches. These stretches may differentiate SVDV from coxsackieviruses.

Dynamics of picornavirus RNA replication within infected cells

“…These viruses can cause clinically indistinguishable diseases in swine; however, FMDV has a much broader host-range and is responsible for one of the most economically important diseases of farm animals. SVDV is closely related both antigenically and genetically to coxsackie virus B5 (Graves, 1973;Inoue et al, 1989).…”

Production of Infectious Swine Vesicular Disease Virus from Cloned cDNA in Mammalian Cells

“…The RNA genome is 7400 nucleotides long, excluding the poly(A) tract and encodes a single polyprotein. The predicted amino acid sequence shows close homology to those of coxsackievirus B and poliovirus (Inoue et al, 1989).…”

“…The constructed full-length SVDV cDNA clone generated infectious virus which was antigenically identical to the parental SVDV. For the construction of full-length SVDV cDNA, clones MPS671, pBRS14, MPS453 and pBRS19 were used (Inoue et al, 1989). The plasmid pUCS825 containing Y-terminal cDNA (nucleotides 1 to 65) was newly prepared by the method of Geliebter (1987) with several modifications.…”

“…In IBRS-2 cells, distinct c.p.e, was observed 2 days after transfection of the plasmids and the infectious viruses were recovered from culture fluids. In Cos-7 cells, (Inoue et al, 1989). DNA fragments indicated by thick lines in each plasmid were ligated using the common restriction sites as indicated.…”

Swine vesicular disease, studies on pathogenesis, diagnosis, and epizootiology: A review

“…The genome of SVDV consists of approximately 7400 nucleotides, and encodes for a single polyprotein of 2815 amino acids (27). This polyprotein is post-translationally cleaved into 11 proteins.…”